Restriction map for
pF1KB7886
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# NO CUT ENZYMES ( 120 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcyI AflII AgeI AhdI Alw44I
AocI Aor51HI ApaI ApaLI AscI
AseI AsnI Asp718I AspEI AspHI
AviII AvrII BalI BamHI BbeI
BbrPI BbvCI BcgI BciVI BfrI
BglI BlnI BlpI Bpu1102I BsaAI
BsaHI BsaI BsaWI BseAI BsiHKAI
BsiWI BsmBI BspDI BspEI BspHI
BspMI BsrDI BsrFI BssHII BssSI
Bst1107I BstAPI BstEII BstPI BstXI
BstZ17I Bsu36I CelII Cfr10I ClaI
CpoI DraI DraIII DrdI Eam1105I
Eco47III Eco81I EcoNI EcoO65I EcoRV
FseI FspI Hin1I KasI KpnI
KspI MfeI MluNI MroI MscI
MunI NaeI NarI NdeI NgoMIV
NheI PacI PaeR7I PflMI PinAI
PmaCI PmeI PmlI PshBI Psp1406I
PvuI RcaI RsrII SacI SacII
SalI SbfI ScaI SexAI SfiI
SfoI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspI StuI SwaI
Van91I XbaI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 41 enzymes) #
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AspI 434 GACCGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 473 CCCGAG C^[CT]CG[GA]G
BanI 6 GGCACC G^G[CT][GA]CC
BanII 1914 GGGCTC G[GA]GC[CT]^C
BclI 479 TGATCA T^GATCA
BseRI 1634 GAGGAG GAGGAG(10/8)
BsiEI 41 CGGCCG CG[GA][CT]^CG
BsoBI 473 CCCGAG C^[CT]CG[GA]G
BspLU11I 553 ACATGT A^CATGT
BstBI 644 TTCGAA TT^CGAA
DraII 1172 AGGACCC [GA]G^G[ACGT]CC[CT]
EaeI 41 CGGCCG [CT]^GGCC[GA]
EagI 41 CGGCCG C^GGCCG
EclXI 41 CGGCCG C^GGCCG
Eco52I 41 CGGCCG C^GGCCG
EcoO109I 1172 AGGACCC [GA]G^G[ACGT]CC[CT]
EcoRI 280 GAATTC G^AATTC
EcoT22I 770 ATGCAT ATGCA^T
FauI 1662 CCCGC CCCGC(4/6)
FbaI 479 TGATCA T^GATCA
HaeII 935 AGCGCC [GA]GCGC^[CT]
HaeIII 42 GGCC GG^CC
HgaI 76 GCGTC GACGC(5/10)
HindIII 1224 AAGCTT A^AGCTT
HphI 196 GGTGA GGTGA(8/7)
MaeII 674 ACGT A^CGT
MluI 22 ACGCGT A^CGCGT
MwoI 1375 GCCTGCCAAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NcoI 1356 CCATGG C^CATGG
NotI 40 GCGGCCGC GC^GGCCGC
NruI 1032 TCGCGA TCG^CGA
NsiI 770 ATGCAT ATGCA^T
NspV 644 TTCGAA TT^CGAA
PflFI 434 GACCGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI 1172 AGGACCC [GA]G^G[AT]CC[CT]
PstI 56 CTGCAG CTGCA^G
PvuII 229 CAGCTG CAG^CTG
SapI 1759 GAAGAGC GCTCTTC(1/4)
SfuI 644 TTCGAA TT^CGAA
Tth111I 434 GACCGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 592 CCATCGACACCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 40 enzymes) #
###################################
AflIII 22 ACGCGT A^C[GA][CT]GT
553 ACATGT
Asp700I 628 GAAAAATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1895 GAAGTCCTTC
BbsI 1744 GAAGAC GAAGAC(2/6)
1567 GTCTTC
BcnI 31 CCGGG CC^[GC]GG
776 CCCGG
BfaI 277 CTAG C^TAG
1276 CTAG
BglII 1278 AGATCT A^GATCT
1649 AGATCT
BmyI 1837 GTGCCC G[AGT]GC[ACT]^C
1914 GGGCTC
BpmI 730 CTGGAG CTGGAG(16/14)
1631 CTGGAG
BpuAI 1744 GAAGAC GAAGAC(2/6)
1567 GTCTTC
BsmAI 1637 GAGAC GTCTC(1/5)
440 GTCTC
BsmFI 826 GGGAC GGGAC(10/14)
1946 GTCCC
BsmI 768 GAATGC GAATGC(1/-1)
1336 GCATTC
Bsp1286I 1837 GTGCCC G[AGT]GC[ACT]^C
1914 GGGCTC
Bsp1407I 1089 TGTACA T^GTACA
1931 TGTACA
BsrBI 1425 GAGCGG CCGCTC(-3/-3)
1474 GAGCGG
BsrGI 1089 TGTACA T^GTACA
1931 TGTACA
BstNI 939 CCTGG CC^[AT]GG
1317 CCAGG
CfoI 74 GCGC GCG^C
936 GCGC
EcoRII 939 CCTGG ^CC[AT]GG
1317 CCAGG
EcoT14I 349 CCTTGG C^C[AT][AT]GG
1356 CCATGG
HapII 31 CCGG C^CGG
777 CCGG
HhaI 74 GCGC GCG^C
936 GCGC
HinP1I 74 GCGC G^CGC
936 GCGC
HincII 1288 GTTAAC GT[CT]^[GA]AC
1528 GTTAAC
HindII 1288 GTTAAC GT[CT]^[GA]AC
1528 GTTAAC
HpaI 1288 GTTAAC GTT^AAC
1528 GTTAAC
HpaII 31 CCGG C^CGG
777 CCGG
MaeI 277 CTAG C^TAG
1276 CTAG
MslI 662 CACTGGAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
945 CATCCTGATG
MspI 31 CCGG C^CGG
777 CCGG
MvaI 939 CCTGG CC^[AT]GG
1317 CCAGG
NciI 31 CCGGG CC^[GC]GG
776 CCCGG
NspI 553 ACATGT [GA]CATG^[CT]
1934 ACATGC
PleI 1639 GACTC GAGTC(4/5)
1461 GAGTC
PshAI 168 GACAGAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
550 GACACATGTC
SmlI 714 CTTGAG C^T[CT][GA]AG
754 CTCAAG
SspBI 1089 TGTACA T^GTACA
1931 TGTACA
StyI 349 CCTTGG C^C[AT][AT]GG
1356 CCATGG
Tsp45I 166 GTGAC ^GT[GC]AC
557 GTCAC
XmnI 628 GAAAAATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1895 GAAGTCCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 57 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
AlwI AlwNI ApoI AvaII BbvI
BsaBI BsaJI BsgI BsiYI BslI
BsrI BssKI BstF5I BstUI BstYI
Cac8I Cfr13I DdeI DpnII DsaI
EarI Fnu4HI FokI HinfI ItaI
Ksp632I MaeIII MamI MboI MboII
MflI MnlI MseI MspA1I MvnI
NdeII NlaIII NlaIV RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI TaqI
TfiI Tru9I TseI Tsp509I TspRI
TthHB8I XhoII