Restriction map for
pF1KB5798
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# NO CUT ENZYMES ( 78 enzymes) #
##################################
AatII AccI AclI AflII AgeI
Alw44I ApaLI AscI AseI AsnI
Asp700I AvrII BbvCI BclI BfrI
BglI BglII BlnI BlpI Bpu1102I
BsaBI BsmBI BsmI Bsp1407I BspDI
BspHI BspLU11I BsrDI BsrGI BssHII
BssSI Bst1107I BstZ17I CelII ClaI
CpoI DraI DrdI EcoRI EcoRV
EcoT22I FbaI FseI HindIII HpaI
MamI MfeI MluI MunI NaeI
NcoI NgoMIV NheI NotI NruI
NsiI PacI PaeR7I PinAI PmeI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SalI SexAI SfiI SgrAI
SnaBI SpeI SphI SspBI SwaI
XcmI XhoI XmnI
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# ONE CUT ENZYMES ( 60 enzymes) #
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Acc65I 973 GGTACC G^GTACC
AccIII 846 TCCGGA T^CCGGA
AflIII 2278 ACACGT A^C[GA][CT]GT
AhdI 2310 GACTATCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 1031 CCTCAGG CC^T[ACGT]AGG
Aor51HI 1368 AGCGCT AGC^GCT
ApaI 2248 GGGCCC GGGCC^C
Asp718I 973 GGTACC G^GTACC
AspEI 2310 GACTATCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 2040 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 608 TGCGCA TGC^GCA
BalI 158 TGGCCA TGG^CCA
BbrPI 2279 CACGTG CAC^GTG
BbsI 354 GAAGAC GAAGAC(2/6)
BciVI 1504 GTATCC GTATCC(6/5)
BpuAI 354 GAAGAC GAAGAC(2/6)
BsaAI 2279 CACGTG [CT]AC^GT[GA]
BsaI 959 GAGACC GGTCTC(1/5)
BseAI 846 TCCGGA T^CCGGA
BsiEI 397 CGGCCG CG[GA][CT]^CG
BsiWI 401 CGTACG C^GTACG
BspEI 846 TCCGGA T^CCGGA
BsrBI 1041 CCGCTC CCGCTC(-3/-3)
BsrFI 147 GCCGGT [GA]^CCGG[CT]
BstBI 685 TTCGAA TT^CGAA
Bsu36I 1031 CCTCAGG CC^T[ACGT]AGG
Cfr10I 147 GCCGGT [GA]^CCGG[CT]
DraIII 1219 CACCGGGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 626 CCGCGG C^C[GA][CT]GG
EagI 397 CGGCCG C^GGCCG
Eam1105I 2310 GACTATCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 397 CGGCCG C^GGCCG
Eco47III 1368 AGCGCT AGC^GCT
Eco52I 397 CGGCCG C^GGCCG
Eco81I 1031 CCTCAGG CC^T[ACGT]AGG
EcoNI 1134 CCTGAAGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FspI 608 TGCGCA TGC^GCA
HincII 967 GTTGAC GT[CT]^[GA]AC
HindII 967 GTTGAC GT[CT]^[GA]AC
KpnI 973 GGTACC GGTAC^C
KspI 626 CCGCGG CCGC^GG
MluNI 158 TGGCCA TGG^CCA
MroI 846 TCCGGA T^CCGGA
MscI 158 TGGCCA TGG^CCA
MseI 1793 TTAA T^TAA
NdeI 1490 CATATG CA^TATG
NspI 1785 ACATGC [GA]CATG^[CT]
NspV 685 TTCGAA TT^CGAA
PflFI 2040 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 2279 CACGTG CAC^GTG
PmlI 2279 CACGTG CAC^GTG
PpuMI 215 AGGTCCT [GA]G^G[AT]CC[CT]
SacI 253 GAGCTC GAGCT^C
SacII 626 CCGCGG CCGC^GG
ScaI 1715 AGTACT AGT^ACT
SfuI 685 TTCGAA TT^CGAA
SspI 278 AATATT AAT^ATT
Tru9I 1793 TTAA T^TAA
Tth111I 2040 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 786 TCTAGA T^CTAGA
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# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AcsI 773 AAATTC [GA]^AATT[CT]
1265 AAATTC
ApoI 773 AAATTC [GA]^AATT[CT]
1265 AAATTC
BamHI 116 GGATCC G^GATCC
1994 GGATCC
BbeI 655 GGCGCC GGCGC^C
756 GGCGCC
BcgI 2117 CGACCCGCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
677 GCAGGCCCTTCG
BsaWI 846 TCCGGA [AT]^CCGG[AT]
907 ACCGGA
BstAPI 213 GCAGGTCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1377 GCAGCGGGTGC
EcoT14I 1212 CCAAGG C^C[AT][AT]GG
1553 CCAAGG
KasI 655 GGCGCC G^GCGCC
756 GGCGCC
MaeII 1457 ACGT A^CGT
2280 ACGT
MslI 90 CACCAGTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
2157 CATCTTCGTG
NarI 655 GGCGCC GG^CGCC
756 GGCGCC
PflMI 2050 CCAGAGAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2320 CCAGTGGGTGG
SbfI 210 CCTGCAGG CCTGCA^GG
569 CCTGCAGG
SfoI 655 GGCGCC GGC^GCC
756 GGCGCC
SmlI 727 CTTGAG C^T[CT][GA]AG
1649 CTCAAG
Sse8387I 210 CCTGCAGG CCTGCA^GG
569 CCTGCAGG
StuI 491 AGGCCT AGG^CCT
1028 AGGCCT
StyI 1212 CCAAGG C^C[AT][AT]GG
1553 CCAAGG
Tsp509I 774 AATT ^AATT
1266 AATT
Van91I 2050 CCAGAGAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2320 CCAGTGGGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 99 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AlwNI AspHI AvaI AvaII
BanI BanII BbvI BcnI BfaI
BmyI BpmI BsaHI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BspMI BsrI
BssKI BstEII BstF5I BstNI BstPI
BstUI BstXI BstYI Cac8I CfoI
Cfr13I DdeI DpnII DraII EaeI
EarI EcoO109I EcoO65I EcoRII FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeIII MboI MboII MflI MnlI
MspA1I MspI MvaI MvnI MwoI
NciI NdeII NlaIII NlaIV PleI
PstI PvuII RsaI SapI Sau3AI
Sau96I ScrFI SfaNI SfcI SmaI
TaqI TfiI TseI Tsp45I TspRI
TthHB8I XhoII XmaCI XmaI