Restriction map for
pF1KB5897
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# NO CUT ENZYMES ( 97 enzymes) #
##################################
AatII Acc65I AccIII AclI AflII
AflIII AgeI AhdI Alw44I Aor51HI
ApaLI AscI AseI AsnI Asp718I
AspEI AspI AvrII BalI BbeI
BcgI BfrI BlnI BsaBI BseAI
BsiEI BsiWI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrDI BsrGI Bst1107I
BstBI BstEII BstPI BstZ17I ClaI
CpoI DraI DraIII DrdI EagI
Eam1105I EclXI Eco47III Eco52I EcoO65I
EcoRI EcoRV FseI HincII HindII
HpaI KasI KpnI MamI MfeI
MluI MluNI MroI MscI MunI
NarI NdeI NheI NotI NspV
PacI PaeR7I PflFI PinAI PmeI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SalI SapI SbfI SexAI
SfoI SfuI SgrAI SnaBI SpeI
Sse8387I SspBI SwaI Tth111I XbaI
XcmI XhoI
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# ONE CUT ENZYMES ( 50 enzymes) #
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AccI 2065 GTCTAC GT^[AC][GT]AC
AcsI 1072 AAATTC [GA]^AATT[CT]
AcyI 1524 GACGCC G[GA]^CG[CT]C
AocI 1603 CCTAAGG CC^T[ACGT]AGG
ApaI 184 GGGCCC GGGCC^C
ApoI 1072 AAATTC [GA]^AATT[CT]
AviII 39 TGCGCA TGC^GCA
BamHI 222 GGATCC G^GATCC
BbrPI 898 CACGTG CAC^GTG
BbvCI 844 GCTGAGG CCTCAGC(-5/-2)
BciVI 472 GTATCC GTATCC(6/5)
BclI 1658 TGATCA T^GATCA
BfaI 316 CTAG C^TAG
BglI 1444 GCCAATGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 1097 AGATCT A^GATCT
BlpI 2076 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 2076 GCTCAGC GC^T[ACGT]AGC
BsaHI 1524 GACGCC G[GA]^CG[CT]C
BsaI 836 GGTCTC GGTCTC(1/5)
BsaWI 965 TCCGGT [AT]^CCGG[AT]
BsmBI 1426 GAGACG CGTCTC(1/5)
BsmI 2129 GCATTC GAATGC(1/-1)
BssSI 1558 CTCGTG CACGAG(-5/-1)
BstAPI 696 GCAGGGTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Bsu36I 1603 CCTAAGG CC^T[ACGT]AGG
CelII 2076 GCTCAGC GC^T[ACGT]AGC
Eco81I 1603 CCTAAGG CC^T[ACGT]AGG
EcoNI 1282 CCTATGCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT22I 621 ATGCAT ATGCA^T
FbaI 1658 TGATCA T^GATCA
FspI 39 TGCGCA TGC^GCA
Hin1I 1524 GACGCC G[GA]^CG[CT]C
KspI 1485 CCGCGG CCGC^GG
MaeI 316 CTAG C^TAG
MseI 794 TTAA T^TAA
NcoI 1364 CCATGG C^CATGG
NruI 2412 TCGCGA TCG^CGA
NsiI 621 ATGCAT ATGCA^T
PflMI 2243 CCAGTGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 898 CACGTG CAC^GTG
PmlI 898 CACGTG CAC^GTG
SacI 766 GAGCTC GAGCT^C
SacII 1485 CCGCGG CCGC^GG
ScaI 2438 AGTACT AGT^ACT
SfiI 1443 GGCCAATGCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SphI 1598 GCATGC GCATG^C
SspI 688 AATATT AAT^ATT
StuI 2496 AGGCCT AGG^CCT
Tru9I 794 TTAA T^TAA
Van91I 2243 CCAGTGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 12 enzymes) #
###################################
Asp700I 540 GAATCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2466 GAAGTTCTTC
AspHI 451 GAGCAC G[AT]GC[AT]^C
766 GAGCTC
BsiHKAI 451 GAGCAC G[AT]GC[AT]^C
766 GAGCTC
BssHII 255 GCGCGC G^CGCGC
294 GCGCGC
BstXI 1364 CCATGGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2453 CCAGCGCCCTGG
DsaI 1364 CCATGG C^C[GA][CT]GG
1485 CCGCGG
HaeII 1481 AGCGCC [GA]GCGC^[CT]
2455 AGCGCC
HindIII 304 AAGCTT A^AGCTT
805 AAGCTT
NaeI 163 GCCGGC GCC^GGC
989 GCCGGC
NgoMIV 163 GCCGGC G^CCGGC
989 GCCGGC
NspI 1598 GCATGC [GA]CATG^[CT]
1697 GCATGT
XmnI 540 GAATCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2466 GAAGTTCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 99 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AlwNI AvaI AvaII BanI BanII
BbsI BbvI BcnI BmyI BpmI
BpuAI BsaAI BsaJI BseRI BsgI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BspMI BsrBI BsrFI BsrI
BssKI BstF5I BstNI BstUI BstYI
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DraII EaeI EarI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeIII HapII HgaI HhaI HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeII MaeIII MboI MboII MflI
MnlI MslI MspA1I MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV PleI PpuMI PstI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmaI SmlI StyI TaqI
TfiI TseI Tsp45I Tsp509I TspRI
TthHB8I XhoII XmaCI XmaI