##################################
# NO CUT ENZYMES ( 174 enzymes) #
##################################
AatII Acc65I AccI AccII AclI
AcsI AcyI AfaI AflII AflIII
AgeI AhdI Alw44I AlwNI AocI
Aor51HI ApaI ApaLI ApoI AscI
AseI AsnI Asp700I Asp718I AspEI
AspI AvaI AvaII AviII AvrII
BalI BamHI BbeI BbrPI BbsI
BbvCI BcgI BciVI BclI BfrI
BlnI BpmI BpuAI BsaAI BsaBI
BsaHI BsaI BsiEI BsiWI BsmBI
BsmI BsoBI Bsp1407I BspDI BspHI
BspLU11I BsrBI BsrDI BsrFI BsrGI
BssHII BssSI Bst1107I BstBI BstEII
BstPI BstUI BstXI BstZ17I Bsu36I
Cfr10I Cfr13I ClaI CpoI DraI
DraII DraIII DsaI EaeI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoNI EcoO109I EcoO65I EcoRI EcoRV
EcoT14I EcoT22I FauI FbaI FseI
FspI Hin1I HincII HindII HindIII
HpaI KasI KpnI KspI MaeII
MamI MfeI MluI MluNI MscI
MslI MspA1I MunI MvnI NaeI
NarI NcoI NdeI NgoMIV NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PflMI PinAI
PleI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PvuI PvuII
RcaI RsaI RsrII SacI SacII
SalI Sau96I SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SmaI
SmlI SnaBI SpeI SphI Sse8387I
SspBI SspI StyI SwaI Tsp509I
TspRI Tth111I Van91I XbaI XcmI
XhoI XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 51 enzymes) #
###################################
AccIII 89 TCCGGA T^CCGGA
AluI 460 AGCT AG^CT
AlwI 324 GGATC GGATC(4/5)
AspHI 335 GTGCTC G[AT]GC[AT]^C
BanI 139 GGCACC G^G[CT][GA]CC
BanII 15 GAGCCC G[GA]GC[CT]^C
BcnI 149 CCCGG CC^[GC]GG
BglI 27 GCCGAAGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 363 AGATCT A^GATCT
BlpI 337 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 337 GCTCAGC GC^T[ACGT]AGC
BsaWI 89 TCCGGA [AT]^CCGG[AT]
BseAI 89 TCCGGA T^CCGGA
BseRI 506 GAGGAG GAGGAG(10/8)
BsgI 81 CTGCAC GTGCAG(16/14)
BsiHKAI 335 GTGCTC G[AT]GC[AT]^C
BsiYI 143 CCTCCACCCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 143 CCTCCACCCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmFI 68 GGGAC GGGAC(10/14)
BspEI 89 TCCGGA T^CCGGA
BspMI 186 GCAGGT ACCTGC(4/8)
BstF5I 54 GGATG GGATG(2/0)
BstYI 363 AGATCT [GA]^GATC[CT]
Cac8I 411 GCCTGC GC[ACGT]^[ACGT]GC
CelII 337 GCTCAGC GC^T[ACGT]AGC
CfoI 98 GCGC GCG^C
DrdI 70 GACTGTAGCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 12 GAAGAG CTCTTC(1/4)
FokI 54 GGATG GGATG(9/13)
HaeII 97 AGCGCC [GA]GCGC^[CT]
HgaI 77 GCGTC GACGC(5/10)
HhaI 98 GCGC GCG^C
HinP1I 98 GCGC G^CGC
HinfI 532 GAATC G^A[ACGT]TC
Ksp632I 12 GAAGAG CTCTTC(1/4)
MflI 363 AGATCT [GA]^GATC[CT]
MroI 89 TCCGGA T^CCGGA
MseI 389 TTAA T^TAA
NciI 149 CCCGG CC^[GC]GG
NlaIII 117 CATG CATG^
NlaIV 139 GGCACC GG[ACGT]^[ACGT]CC
PstI 431 CTGCAG CTGCA^G
SapI 12 GAAGAGC GCTCTTC(1/4)
SfaNI 415 GCATC GCATC(5/9)
StuI 104 AGGCCT AGG^CCT
TaqI 159 TCGA T^CGA
TfiI 532 GAATC G^A[AT]TC
Tru9I 389 TTAA T^TAA
Tsp45I 405 GTCAC ^GT[GC]AC
TthHB8I 159 TCGA T^CGA
XhoII 363 AGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 12 enzymes) #
###################################
BbvI 36 GCAGC GCAGC(8/12)
424 GCTGC
BsaJI 50 CCTCGG C^C[ACGT][ACGT]GG
101 CCGAGG
BsmAI 5 GAGAC GTCTC(1/5)
166 GTCTC
BsrI 65 ACTGG ACTGG(1/-1)
171 CCAGT
DdeI 194 CTCAG C^T[ACGT]AG
338 CTCAG
HapII 90 CCGG C^CGG
150 CCGG
HpaII 90 CCGG C^CGG
150 CCGG
HphI 406 TCACC GGTGA(8/7)
499 TCACC
MboII 12 GAAGA GAAGA(8/7)
496 TCTTC
MspI 90 CCGG C^CGG
150 CCGG
SfcI 72 CTGTAG C^T[GA][CT]AG
431 CTGCAG
TseI 36 GCAGC G^C[AT]GC
424 GCTGC
#########################################
# 3 OR MORE CUT ENZYMES ( 21 enzymes) #
#########################################
AciI BfaI BmyI Bsp1286I BssKI
BstAPI BstNI DpnII EcoRII Fnu4HI
HaeIII ItaI MaeI MaeIII MboI
MnlI MvaI MwoI NdeII Sau3AI
ScrFI