Restriction map for pF1KB7919
##################################
# NO CUT ENZYMES (  174 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AclI        
AcsI        AcyI        AfaI        AflII       AflIII      
AgeI        AhdI        Alw44I      AlwNI       AocI        
Aor51HI     ApaI        ApaLI       ApoI        AscI        
AseI        AsnI        Asp700I     Asp718I     AspEI       
AspI        AvaI        AvaII       AviII       AvrII       
BalI        BamHI       BbeI        BbrPI       BbsI        
BbvCI       BcgI        BciVI       BclI        BfrI        
BlnI        BpmI        BpuAI       BsaAI       BsaBI       
BsaHI       BsaI        BsiEI       BsiWI       BsmBI       
BsmI        BsoBI       Bsp1407I    BspDI       BspHI       
BspLU11I    BsrBI       BsrDI       BsrFI       BsrGI       
BssHII      BssSI       Bst1107I    BstBI       BstEII      
BstPI       BstUI       BstXI       BstZ17I     Bsu36I      
Cfr10I      Cfr13I      ClaI        CpoI        DraI        
DraII       DraIII      DsaI        EaeI        EagI        
Eam1105I    EclXI       Eco47III    Eco52I      Eco81I      
EcoNI       EcoO109I    EcoO65I     EcoRI       EcoRV       
EcoT14I     EcoT22I     FauI        FbaI        FseI        
FspI        Hin1I       HincII      HindII      HindIII     
HpaI        KasI        KpnI        KspI        MaeII       
MamI        MfeI        MluI        MluNI       MscI        
MslI        MspA1I      MunI        MvnI        NaeI        
NarI        NcoI        NdeI        NgoMIV      NheI        
NotI        NruI        NsiI        NspI        NspV        
PacI        PaeR7I      PflFI       PflMI       PinAI       
PleI        PmaCI       PmeI        PmlI        PpuMI       
PshAI       PshBI       Psp1406I    PvuI        PvuII       
RcaI        RsaI        RsrII       SacI        SacII       
SalI        Sau96I      SbfI        ScaI        SexAI       
SfiI        SfoI        SfuI        SgrAI       SmaI        
SmlI        SnaBI       SpeI        SphI        Sse8387I    
SspBI       SspI        StyI        SwaI        Tsp509I     
TspRI       Tth111I     Van91I      XbaI        XcmI        
XhoI        XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   51 enzymes) #
###################################

AccIII         89  TCCGGA               T^CCGGA
AluI          460  AGCT                 AG^CT
AlwI          324  GGATC                GGATC(4/5)
AspHI         335  GTGCTC               G[AT]GC[AT]^C
BanI          139  GGCACC               G^G[CT][GA]CC
BanII          15  GAGCCC               G[GA]GC[CT]^C
BcnI          149  CCCGG                CC^[GC]GG
BglI           27  GCCGAAGCGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII         363  AGATCT               A^GATCT
BlpI          337  GCTCAGC              GC^T[ACGT]AGC
Bpu1102I      337  GCTCAGC              GC^T[ACGT]AGC
BsaWI          89  TCCGGA               [AT]^CCGG[AT]
BseAI          89  TCCGGA               T^CCGGA
BseRI         506  GAGGAG               GAGGAG(10/8)
BsgI           81  CTGCAC               GTGCAG(16/14)
BsiHKAI       335  GTGCTC               G[AT]GC[AT]^C
BsiYI         143  CCTCCACCCGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI          143  CCTCCACCCGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmFI          68  GGGAC                GGGAC(10/14)
BspEI          89  TCCGGA               T^CCGGA
BspMI         186  GCAGGT               ACCTGC(4/8)
BstF5I         54  GGATG                GGATG(2/0)
BstYI         363  AGATCT               [GA]^GATC[CT]
Cac8I         411  GCCTGC               GC[ACGT]^[ACGT]GC
CelII         337  GCTCAGC              GC^T[ACGT]AGC
CfoI           98  GCGC                 GCG^C
DrdI           70  GACTGTAGCGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI           12  GAAGAG               CTCTTC(1/4)
FokI           54  GGATG                GGATG(9/13)
HaeII          97  AGCGCC               [GA]GCGC^[CT]
HgaI           77  GCGTC                GACGC(5/10)
HhaI           98  GCGC                 GCG^C
HinP1I         98  GCGC                 G^CGC
HinfI         532  GAATC                G^A[ACGT]TC
Ksp632I        12  GAAGAG               CTCTTC(1/4)
MflI          363  AGATCT               [GA]^GATC[CT]
MroI           89  TCCGGA               T^CCGGA
MseI          389  TTAA                 T^TAA
NciI          149  CCCGG                CC^[GC]GG
NlaIII        117  CATG                 CATG^
NlaIV         139  GGCACC               GG[ACGT]^[ACGT]CC
PstI          431  CTGCAG               CTGCA^G
SapI           12  GAAGAGC              GCTCTTC(1/4)
SfaNI         415  GCATC                GCATC(5/9)
StuI          104  AGGCCT               AGG^CCT
TaqI          159  TCGA                 T^CGA
TfiI          532  GAATC                G^A[AT]TC
Tru9I         389  TTAA                 T^TAA
Tsp45I        405  GTCAC                ^GT[GC]AC
TthHB8I       159  TCGA                 T^CGA
XhoII         363  AGATCT               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   12 enzymes) #
###################################

BbvI           36  GCAGC                GCAGC(8/12)
              424  GCTGC               
BsaJI          50  CCTCGG               C^C[ACGT][ACGT]GG
              101  CCGAGG              
BsmAI           5  GAGAC                GTCTC(1/5)
              166  GTCTC               
BsrI           65  ACTGG                ACTGG(1/-1)
              171  CCAGT               
DdeI          194  CTCAG                C^T[ACGT]AG
              338  CTCAG               
HapII          90  CCGG                 C^CGG
              150  CCGG                
HpaII          90  CCGG                 C^CGG
              150  CCGG                
HphI          406  TCACC                GGTGA(8/7)
              499  TCACC               
MboII          12  GAAGA                GAAGA(8/7)
              496  TCTTC               
MspI           90  CCGG                 C^CGG
              150  CCGG                
SfcI           72  CTGTAG               C^T[GA][CT]AG
              431  CTGCAG              
TseI           36  GCAGC                G^C[AT]GC
              424  GCTGC               


#########################################
# 3 OR MORE CUT ENZYMES (   21 enzymes) #
#########################################

AciI        BfaI        BmyI        Bsp1286I    BssKI       
BstAPI      BstNI       DpnII       EcoRII      Fnu4HI      
HaeIII      ItaI        MaeI        MaeIII      MboI        
MnlI        MvaI        MwoI        NdeII       Sau3AI      
ScrFI       
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com