################################## # NO CUT ENZYMES ( 174 enzymes) # ################################## AatII Acc65I AccI AccII AclI AcsI AcyI AfaI AflII AflIII AgeI AhdI Alw44I AlwNI AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvaI AvaII AviII AvrII BalI BamHI BbeI BbrPI BbsI BbvCI BcgI BciVI BclI BfrI BlnI BpmI BpuAI BsaAI BsaBI BsaHI BsaI BsiEI BsiWI BsmBI BsmI BsoBI Bsp1407I BspDI BspHI BspLU11I BsrBI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstBI BstEII BstPI BstUI BstXI BstZ17I Bsu36I Cfr10I Cfr13I ClaI CpoI DraI DraII DraIII DsaI EaeI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRV EcoT14I EcoT22I FauI FbaI FseI FspI Hin1I HincII HindII HindIII HpaI KasI KpnI KspI MaeII MamI MfeI MluI MluNI MscI MslI MspA1I MunI MvnI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PleI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI PvuII RcaI RsaI RsrII SacI SacII SalI Sau96I SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StyI SwaI Tsp509I TspRI Tth111I Van91I XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 51 enzymes) # ################################### AccIII 89 TCCGGA T^CCGGA AluI 460 AGCT AG^CT AlwI 324 GGATC GGATC(4/5) AspHI 335 GTGCTC G[AT]GC[AT]^C BanI 139 GGCACC G^G[CT][GA]CC BanII 15 GAGCCC G[GA]GC[CT]^C BcnI 149 CCCGG CC^[GC]GG BglI 27 GCCGAAGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BglII 363 AGATCT A^GATCT BlpI 337 GCTCAGC GC^T[ACGT]AGC Bpu1102I 337 GCTCAGC GC^T[ACGT]AGC BsaWI 89 TCCGGA [AT]^CCGG[AT] BseAI 89 TCCGGA T^CCGGA BseRI 506 GAGGAG GAGGAG(10/8) BsgI 81 CTGCAC GTGCAG(16/14) BsiHKAI 335 GTGCTC G[AT]GC[AT]^C BsiYI 143 CCTCCACCCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BslI 143 CCTCCACCCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BsmFI 68 GGGAC GGGAC(10/14) BspEI 89 TCCGGA T^CCGGA BspMI 186 GCAGGT ACCTGC(4/8) BstF5I 54 GGATG GGATG(2/0) BstYI 363 AGATCT [GA]^GATC[CT] Cac8I 411 GCCTGC GC[ACGT]^[ACGT]GC CelII 337 GCTCAGC GC^T[ACGT]AGC CfoI 98 GCGC GCG^C DrdI 70 GACTGTAGCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EarI 12 GAAGAG CTCTTC(1/4) FokI 54 GGATG GGATG(9/13) HaeII 97 AGCGCC [GA]GCGC^[CT] HgaI 77 GCGTC GACGC(5/10) HhaI 98 GCGC GCG^C HinP1I 98 GCGC G^CGC HinfI 532 GAATC G^A[ACGT]TC Ksp632I 12 GAAGAG CTCTTC(1/4) MflI 363 AGATCT [GA]^GATC[CT] MroI 89 TCCGGA T^CCGGA MseI 389 TTAA T^TAA NciI 149 CCCGG CC^[GC]GG NlaIII 117 CATG CATG^ NlaIV 139 GGCACC GG[ACGT]^[ACGT]CC PstI 431 CTGCAG CTGCA^G SapI 12 GAAGAGC GCTCTTC(1/4) SfaNI 415 GCATC GCATC(5/9) StuI 104 AGGCCT AGG^CCT TaqI 159 TCGA T^CGA TfiI 532 GAATC G^A[AT]TC Tru9I 389 TTAA T^TAA Tsp45I 405 GTCAC ^GT[GC]AC TthHB8I 159 TCGA T^CGA XhoII 363 AGATCT [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 12 enzymes) # ################################### BbvI 36 GCAGC GCAGC(8/12) 424 GCTGC BsaJI 50 CCTCGG C^C[ACGT][ACGT]GG 101 CCGAGG BsmAI 5 GAGAC GTCTC(1/5) 166 GTCTC BsrI 65 ACTGG ACTGG(1/-1) 171 CCAGT DdeI 194 CTCAG C^T[ACGT]AG 338 CTCAG HapII 90 CCGG C^CGG 150 CCGG HpaII 90 CCGG C^CGG 150 CCGG HphI 406 TCACC GGTGA(8/7) 499 TCACC MboII 12 GAAGA GAAGA(8/7) 496 TCTTC MspI 90 CCGG C^CGG 150 CCGG SfcI 72 CTGTAG C^T[GA][CT]AG 431 CTGCAG TseI 36 GCAGC G^C[AT]GC 424 GCTGC ######################################### # 3 OR MORE CUT ENZYMES ( 21 enzymes) # ######################################### AciI BfaI BmyI Bsp1286I BssKI BstAPI BstNI DpnII EcoRII Fnu4HI HaeIII ItaI MaeI MaeIII MboI MnlI MvaI MwoI NdeII Sau3AI ScrFI