Restriction map for
pF1KB7943
##################################
# NO CUT ENZYMES ( 116 enzymes) #
##################################
AatII Acc65I AccI AclI AcsI
AflII AflIII AgeI AhdI Aor51HI
ApoI AscI AseI AsnI Asp700I
Asp718I AspEI AspI AviII AvrII
BamHI BanI BbeI BbrPI BciVI
BclI BfaI BfrI BglII BlnI
BsaAI BsaBI BsiEI BsiWI BsmBI
BsmI BspDI BspHI BspLU11I BsrBI
BsrDI BssHII BssSI Bst1107I BstEII
BstF5I BstPI BstYI BstZ17I ClaI
DraI DraIII DrdI EagI Eam1105I
EclXI Eco47III Eco52I EcoO65I EcoRI
EcoRV EcoT22I FbaI FokI FseI
FspI HindIII HpaI KasI KpnI
KspI MaeI MaeIII MamI MfeI
MflI MluI MseI MunI NaeI
NarI NdeI NgoMIV NheI NotI
NruI NsiI PacI PflFI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI RcaI SacI SacII
SalI SbfI ScaI SfoI SgrAI
SnaBI SpeI Sse8387I SspI SwaI
Tru9I Tsp45I Tth111I XbaI XhoII
XmnI
###################################
# ONE CUT ENZYMES ( 60 enzymes) #
###################################
AccII 1098 CGCG CG^CG
AccIII 362 TCCGGA T^CCGGA
AcyI 901 GGCGTC G[GA]^CG[CT]C
AfaI 60 GTAC GT^AC
AlwI 567 GGATC GGATC(4/5)
AocI 316 CCTGAGG CC^T[ACGT]AGG
ApaI 735 GGGCCC GGGCC^C
BalI 296 TGGCCA TGG^CCA
BbsI 258 GAAGAC GAAGAC(2/6)
BcgI 504 CGACCCCCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BpuAI 258 GAAGAC GAAGAC(2/6)
BsaHI 901 GGCGTC G[GA]^CG[CT]C
BsaI 992 GGTCTC GGTCTC(1/5)
BsaWI 362 TCCGGA [AT]^CCGG[AT]
BseAI 362 TCCGGA T^CCGGA
BsmAI 993 GTCTC GTCTC(1/5)
Bsp1407I 59 TGTACA T^GTACA
BspEI 362 TCCGGA T^CCGGA
BsrFI 110 ACCGGC [GA]^CCGG[CT]
BsrGI 59 TGTACA T^GTACA
BstAPI 961 GCAGCCGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 841 TTCGAA TT^CGAA
BstUI 1098 CGCG CG^CG
BstXI 815 CCAACAACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 316 CCTGAGG CC^T[ACGT]AGG
Cfr10I 110 ACCGGC [GA]^CCGG[CT]
CpoI 575 CGGACCG CG^G[AT]CCG
DpnII 568 GATC ^GATC
DsaI 426 CCATGG C^C[GA][CT]GG
Eco81I 316 CCTGAGG CC^T[ACGT]AGG
HgaI 902 GCGTC GACGC(5/10)
Hin1I 901 GGCGTC G[GA]^CG[CT]C
HincII 904 GTCAAC GT[CT]^[GA]AC
HindII 904 GTCAAC GT[CT]^[GA]AC
MboI 568 GATC ^GATC
MluNI 296 TGGCCA TGG^CCA
MroI 362 TCCGGA T^CCGGA
MscI 296 TGGCCA TGG^CCA
MvnI 1098 CGCG CG^CG
NcoI 426 CCATGG C^CATGG
NdeII 568 GATC ^GATC
NspV 841 TTCGAA TT^CGAA
PaeR7I 232 CTCGAG C^TCGAG
PvuII 466 CAGCTG CAG^CTG
RsaI 60 GTAC GT^AC
RsrII 575 CGGACCG CG^G[AT]CCG
SapI 624 GAAGAGC GCTCTTC(1/4)
Sau3AI 568 GATC ^GATC
SexAI 821 ACCTGGT A^CC[AT]GGT
SfiI 727 GGCCTCCTGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfuI 841 TTCGAA TT^CGAA
SmaI 323 CCCGGG CCC^GGG
SmlI 232 CTCGAG C^T[CT][GA]AG
SspBI 59 TGTACA T^GTACA
StuI 1020 AGGCCT AGG^CCT
TfiI 682 GATTC G^A[AT]TC
Tsp509I 791 AATT ^AATT
XhoI 232 CTCGAG C^TCGAG
XmaCI 323 CCCGGG C^CCGGG
XmaI 323 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
Alw44I 303 GTGCAC G^TGCAC
826 GTGCAC
ApaLI 303 GTGCAC G^TGCAC
826 GTGCAC
AspHI 303 GTGCAC G[AT]GC[AT]^C
826 GTGCAC
BbvCI 415 GCTGAGG CCTCAGC(-5/-2)
604 GCTGAGG
BglI 82 GCCCAGCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
728 GCCTCCTGGGC
BpmI 397 CTCCAG CTGGAG(16/14)
455 CTCCAG
BseRI 995 CTCCTC GAGGAG(10/8)
583 GAGGAG
BsiHKAI 303 GTGCAC G[AT]GC[AT]^C
826 GTGCAC
EaeI 296 TGGCCA [CT]^GGCC[GA]
809 CGGCCA
EarI 772 CTCTTC CTCTTC(1/4)
624 GAAGAG
EcoNI 312 CCTGCCTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
381 CCTGCAGAAGG
EcoT14I 426 CCATGG C^C[AT][AT]GG
674 CCAAGG
FauI 762 CCCGC CCCGC(4/6)
915 CCCGC
HaeII 52 AGCGCC [GA]GCGC^[CT]
143 AGCGCC
Ksp632I 772 CTCTTC CTCTTC(1/4)
624 GAAGAG
MaeII 198 ACGT A^CGT
489 ACGT
MslI 705 CACTCCTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
976 CACCAGGGTG
NspI 96 GCATGC [GA]CATG^[CT]
123 GCATGC
PflMI 718 CCAGGGGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
798 CCAGCACATGG
PpuMI 587 AGGACCC [GA]G^G[AT]CC[CT]
608 AGGACCC
SphI 96 GCATGC GCATG^C
123 GCATGC
StyI 426 CCATGG C^C[AT][AT]GG
674 CCAAGG
Van91I 718 CCAGGGGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
798 CCAGCACATGG
XcmI 701 CCATCACTCCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
812 CCACCAACAACCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 58 enzymes) #
#########################################
AciI AluI AlwNI AvaI AvaII
BanII BbvI BcnI BlpI BmyI
Bpu1102I BsaJI BsgI BsiYI BslI
BsmFI BsoBI Bsp1286I BspMI BsrI
BssKI BstNI Cac8I CelII CfoI
Cfr13I DdeI DraII EcoO109I EcoRII
Fnu4HI HaeIII HapII HhaI HinP1I
HinfI HpaII HphI ItaI MboII
MnlI MspA1I MspI MvaI MwoI
NciI NlaIII NlaIV PleI PstI
Sau96I ScrFI SfaNI SfcI TaqI
TseI TspRI TthHB8I