Restriction map for
pF1KB7988
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# NO CUT ENZYMES ( 144 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AfaI AflII AgeI AhdI
Alw44I AlwI AocI Aor51HI ApaLI
ApoI AscI AseI AsnI Asp700I
Asp718I AspEI AviII AvrII BalI
BamHI BbrPI BbvCI BciVI BclI
BfrI BglII BlnI BlpI BpmI
Bpu1102I BsaAI BsaBI BsaI BseAI
BsiWI BsmI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrBI BsrDI BsrGI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstYI BstZ17I Bsu36I
CelII ClaI DpnII DraI DraIII
DrdI Eam1105I EarI Eco47III Eco81I
EcoNI EcoO65I EcoRI EcoRV EcoT22I
FbaI FseI FspI HincII HindII
HindIII HpaI KpnI Ksp632I KspI
MamI MboI MfeI MflI MluI
MluNI MroI MscI MseI MunI
NdeI NdeII NheI NruI NsiI
NspI NspV PacI PaeR7I PflMI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI RcaI RsaI
SacI SacII SalI SapI Sau3AI
SbfI ScaI SexAI SfiI SfuI
SmlI SnaBI SpeI SphI Sse8387I
SspBI SspI SwaI TaqI TfiI
Tru9I Tsp509I TthHB8I Van91I XbaI
XcmI XhoI XhoII XmnI
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# ONE CUT ENZYMES ( 26 enzymes) #
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AlwNI 700 CAGTCGCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 253 GGGCCC GGGCC^C
AspI 429 GACCGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbeI 135 GGCGCC GGCGC^C
BbsI 489 GAAGAC GAAGAC(2/6)
BcgI 914 GCACCAGCATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BpuAI 489 GAAGAC GAAGAC(2/6)
BsmBI 858 CGTCTC CGTCTC(1/5)
BspMI 444 GCAGGT ACCTGC(4/8)
BstF5I 121 CATCC GGATG(2/0)
CpoI 729 CGGTCCG CG^G[AT]CCG
DdeI 545 CTCAG C^T[ACGT]AG
FokI 121 CATCC GGATG(9/13)
KasI 135 GGCGCC G^GCGCC
MaeIII 651 GTCAC ^GT[ACGT]AC
NarI 135 GGCGCC GG^CGCC
NotI 631 GCGGCCGC GC^GGCCGC
PflFI 429 GACCGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI 128 GGGACCT [GA]G^G[AT]CC[CT]
PvuII 655 CAGCTG CAG^CTG
RsrII 729 CGGTCCG CG^G[AT]CCG
SfoI 135 GGCGCC GGC^GCC
SgrAI 668 CGCCGGCG C[GA]^CCGG[CT]G
StuI 537 AGGCCT AGG^CCT
Tsp45I 651 GTCAC ^GT[GC]AC
Tth111I 429 GACCGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AcyI 25 GACGCC G[GA]^CG[CT]C
135 GGCGCC
AflIII 500 ACGTGT A^C[GA][CT]GT
902 ACGTGT
AspHI 597 GTGCTC G[AT]GC[AT]^C
949 GAGCAC
AvaII 129 GGACC G^G[AT]CC
730 GGTCC
BfaI 34 CTAG C^TAG
946 CTAG
BglI 211 GCCTCGCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
278 GCCAAGCCGGC
BsaHI 25 GACGCC G[GA]^CG[CT]C
135 GGCGCC
BsaWI 320 ACCGGA [AT]^CCGG[AT]
862 TCCGGT
BseRI 360 GAGGAG GAGGAG(10/8)
403 GAGGAG
BsgI 442 GTGCAG GTGCAG(16/14)
714 GTGCAG
BsiEI 632 CGGCCG CG[GA][CT]^CG
725 CGGCCG
BsiHKAI 597 GTGCTC G[AT]GC[AT]^C
949 GAGCAC
BsrI 151 CCAGT ACTGG(1/-1)
711 CCAGT
BstXI 740 CCAACTCCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
758 CCAACAGCCTGG
DraII 128 GGGACCT [GA]G^G[ACGT]CC[CT]
253 GGGCCCC
EagI 632 CGGCCG C^GGCCG
725 CGGCCG
EclXI 632 CGGCCG C^GGCCG
725 CGGCCG
Eco52I 632 CGGCCG C^GGCCG
725 CGGCCG
EcoO109I 128 GGGACCT [GA]G^G[ACGT]CC[CT]
253 GGGCCCC
Hin1I 25 GACGCC G[GA]^CG[CT]C
135 GGCGCC
HinfI 375 GACTC G^A[ACGT]TC
433 GAGTC
MaeI 34 CTAG C^TAG
946 CTAG
MboII 477 GAAGA GAAGA(8/7)
489 GAAGA
MslI 189 CACCACAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
390 CATCTTTATG
NaeI 283 GCCGGC GCC^GGC
669 GCCGGC
NgoMIV 283 GCCGGC G^CCGGC
669 GCCGGC
PleI 375 GACTC GAGTC(4/5)
433 GAGTC
PstI 790 CTGCAG CTGCA^G
879 CTGCAG
SfaNI 46 GATGC GCATC(5/9)
920 GCATC
SfcI 790 CTGCAG C^T[GA][CT]AG
879 CTGCAG
#########################################
# 3 OR MORE CUT ENZYMES ( 58 enzymes) #
#########################################
AccII AciI AluI AvaI BanI
BanII BbvI BcnI BmyI BsaJI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BsrFI BssKI BstNI BstUI
Cac8I CfoI Cfr10I Cfr13I DsaI
EaeI EcoRII EcoT14I FauI Fnu4HI
HaeII HaeIII HapII HgaI HhaI
HinP1I HpaII HphI ItaI MaeII
MnlI MspA1I MspI MvaI MvnI
MwoI NciI NcoI NlaIII NlaIV
Sau96I ScrFI SmaI StyI TseI
TspRI XmaCI XmaI