Restriction map for
pF1KB8511
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# NO CUT ENZYMES ( 88 enzymes) #
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AatII Acc65I AccI AccIII AclI
AflII AflIII AgeI AlwNI AscI
AseI AsnI Asp718I AspI AviII
BbrPI BbsI BbvCI BclI BfrI
BpuAI BseAI BsiWI BsmI Bsp1407I
BspDI BspEI BspLU11I BsrDI BsrGI
Bst1107I BstAPI BstBI BstEII BstPI
BstZ17I ClaI CpoI DraIII DrdI
EcoO65I EcoRI EcoT22I FbaI FspI
HindIII HpaI KpnI KspI MfeI
MluI MroI MunI NdeI NheI
NotI NruI NsiI NspV PacI
PflFI PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PvuI
PvuII RsrII SacII SalI SapI
ScaI SexAI SfuI SgrAI SnaBI
SpeI SphI SspBI SspI StuI
Tth111I XbaI XcmI
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# ONE CUT ENZYMES ( 51 enzymes) #
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AcsI 831 AAATTC [GA]^AATT[CT]
AhdI 791 GACAGACCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 1673 GTGCAC G^TGCAC
AocI 2787 CCTTAGG CC^T[ACGT]AGG
Aor51HI 2696 AGCGCT AGC^GCT
ApaLI 1673 GTGCAC G^TGCAC
ApoI 831 AAATTC [GA]^AATT[CT]
AspEI 791 GACAGACCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvrII 24 CCTAGG C^CTAGG
BcgI 2271 GCAGCTTACTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 2432 GTATCC GTATCC(6/5)
BglI 1900 GCCTTCCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 24 CCTAGG C^CTAGG
BsaAI 49 TACGTG [CT]AC^GT[GA]
BsaBI 1122 GATTTACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 1330 GAGACC GGTCTC(1/5)
BsgI 2708 GTGCAG GTGCAG(16/14)
BsmBI 907 GAGACG CGTCTC(1/5)
BsrBI 1975 GAGCGG CCGCTC(-3/-3)
BsrFI 2800 GCCGGC [GA]^CCGG[CT]
BssHII 1734 GCGCGC G^CGCGC
BssSI 1676 CACGAG CACGAG(-5/-1)
Bsu36I 2787 CCTTAGG CC^T[ACGT]AGG
Cfr10I 2800 GCCGGC [GA]^CCGG[CT]
DraI 828 TTTAAA TTT^AAA
EagI 2802 CGGCCG C^GGCCG
Eam1105I 791 GACAGACCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 2802 CGGCCG C^GGCCG
Eco47III 2696 AGCGCT AGC^GCT
Eco52I 2802 CGGCCG C^GGCCG
Eco81I 2787 CCTTAGG CC^T[ACGT]AGG
EcoNI 697 CCTGGCACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV 868 GATATC GAT^ATC
FseI 2799 GGCCGGCC GGCCGG^CC
HgaI 678 GACGC GACGC(5/10)
HincII 802 GTTGAC GT[CT]^[GA]AC
HindII 802 GTTGAC GT[CT]^[GA]AC
MamI 1122 GATTTACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NaeI 2800 GCCGGC GCC^GGC
NcoI 1758 CCATGG C^CATGG
NgoMIV 2800 GCCGGC G^CCGGC
NspI 1194 GCATGT [GA]CATG^[CT]
SacI 1303 GAGCTC GAGCT^C
SbfI 1698 CCTGCAGG CCTGCA^GG
SfiI 1899 GGCCTTCCAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmaI 2024 CCCGGG CCC^GGG
Sse8387I 1698 CCTGCAGG CCTGCA^GG
SwaI 827 ATTTAAAT ATTT^AAAT
TfiI 1259 GAATC G^A[AT]TC
XmaCI 2024 CCCGGG C^CCGGG
XmaI 2024 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 23 enzymes) #
###################################
ApaI 103 GGGCCC GGGCC^C
1979 GGGCCC
Asp700I 15 GAAACCGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2814 GAACACCTTC
BamHI 600 GGATCC G^GATCC
1517 GGATCC
BanI 70 GGCGCC G^G[CT][GA]CC
1840 GGCGCC
BbeI 70 GGCGCC GGCGC^C
1840 GGCGCC
BfaI 25 CTAG C^TAG
1256 CTAG
BglII 1928 AGATCT A^GATCT
2085 AGATCT
BsaWI 577 ACCGGA [AT]^CCGG[AT]
2428 TCCGGT
BsiEI 1461 CGACCG CG[GA][CT]^CG
2802 CGGCCG
BspHI 995 TCATGA T^CATGA
2033 TCATGA
BspMI 1561 ACCTGC ACCTGC(4/8)
1701 GCAGGT
DraII 2099 AGGCCCT [GA]G^G[ACGT]CC[CT]
2467 AGGCCCC
EcoO109I 2099 AGGCCCT [GA]G^G[ACGT]CC[CT]
2467 AGGCCCC
KasI 70 GGCGCC G^GCGCC
1840 GGCGCC
MaeI 25 CTAG C^TAG
1256 CTAG
MaeII 50 ACGT A^CGT
507 ACGT
MspA1I 511 CAGCGG C[AC]G^C[GT]G
2560 CAGCGG
NarI 70 GGCGCC GG^CGCC
1840 GGCGCC
PflMI 2126 CCAAGCTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2579 CCAGGGAGTGG
RcaI 995 TCATGA T^CATGA
2033 TCATGA
SfoI 70 GGCGCC GGC^GCC
1840 GGCGCC
Van91I 2126 CCAAGCTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2579 CCAGGGAGTGG
XmnI 15 GAAACCGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2814 GAACACCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 96 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AspHI AvaI AvaII BalI
BanII BbvI BcnI BlpI BmyI
BpmI Bpu1102I BsaHI BsaJI BseRI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BsrI BssKI BstF5I
BstNI BstUI BstXI BstYI Cac8I
CelII CfoI Cfr13I DdeI DpnII
DsaI EaeI EarI EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV PaeR7I PleI PstI RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI StyI TaqI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XhoI
XhoII