Restriction map for
pF1KB5866
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# NO CUT ENZYMES ( 155 enzymes) #
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AatII Acc65I AccII AccIII AclI
AcsI AcyI AflII AgeI AhdI
Alw44I AlwNI Aor51HI ApaI ApaLI
ApoI AscI AseI AsnI Asp700I
Asp718I AspEI AspHI AspI AvaI
AviII AvrII BalI BanI BbeI
BbrPI BbvCI BcgI BciVI BclI
BcnI BfrI BglI BlnI BlpI
Bpu1102I BsaBI BsaHI BsaI BsaWI
BseAI BsgI BsiEI BsiHKAI BsiWI
BsmBI BsmI BsoBI BspDI BspEI
BspHI BspMI BsrBI BsrDI BssHII
BssSI BstBI BstEII BstPI BstUI
BstXI CelII CfoI ClaI CpoI
DraI DraIII EaeI EagI Eam1105I
EclXI Eco47III Eco52I EcoNI EcoO65I
EcoRI EcoRV EcoT22I FauI FbaI
FseI FspI HaeII HgaI HhaI
Hin1I HinP1I HindIII KasI KpnI
KspI MamI MfeI MluI MluNI
MroI MscI MslI MunI MvnI
NaeI NarI NciI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PflMI
PinAI PleI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PstI PvuI
RcaI RsrII SacI SacII SalI
SapI SbfI SfiI SfoI SfuI
SgrAI SmaI SmlI SnaBI SpeI
SphI Sse8387I SwaI Tth111I Van91I
XbaI XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 41 enzymes) #
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AccI 802 GTATAC GT^[AC][GT]AC
AciI 190 CCGC CCGC(-3/-1)
AflIII 440 ACATGT A^C[GA][CT]GT
AocI 174 CCTGAGG CC^T[ACGT]AGG
BamHI 888 GGATCC G^GATCC
BanII 215 GGGCTC G[GA]GC[CT]^C
BfaI 79 CTAG C^TAG
BglII 1106 AGATCT A^GATCT
BpmI 275 CTGGAG CTGGAG(16/14)
BsaAI 1218 CACGTA [CT]AC^GT[GA]
Bsp1407I 1080 TGTACA T^GTACA
BspLU11I 440 ACATGT A^CATGT
BsrFI 1135 GCCGGT [GA]^CCGG[CT]
BsrGI 1080 TGTACA T^GTACA
Bst1107I 802 GTATAC GTA^TAC
BstAPI 671 GCACCCCATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstZ17I 802 GTATAC GTA^TAC
Bsu36I 174 CCTGAGG CC^T[ACGT]AGG
Cfr10I 1135 GCCGGT [GA]^CCGG[CT]
DrdI 778 GACAGTTCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 129 CCGTGG C^C[GA][CT]GG
Eco81I 174 CCTGAGG CC^T[ACGT]AGG
EcoT14I 90 CCAAGG C^C[AT][AT]GG
HapII 1136 CCGG C^CGG
HpaI 1245 GTTAAC GTT^AAC
HpaII 1136 CCGG C^CGG
MaeI 79 CTAG C^TAG
MaeII 1219 ACGT A^CGT
MaeIII 809 GTGAC ^GT[ACGT]AC
MspI 1136 CCGG C^CGG
PpuMI 1005 GGGACCC [GA]G^G[AT]CC[CT]
ScaI 512 AGTACT AGT^ACT
SexAI 954 ACCAGGT A^CC[AT]GGT
SfcI 320 CTACAG C^T[GA][CT]AG
SspBI 1080 TGTACA T^GTACA
StuI 178 AGGCCT AGG^CCT
StyI 90 CCAAGG C^C[AT][AT]GG
TaqI 138 TCGA T^CGA
Tsp45I 809 GTGAC ^GT[GC]AC
TthHB8I 138 TCGA T^CGA
XcmI 955 CCAGGTGAATATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 13 enzymes) #
###################################
AvaII 1006 GGACC G^G[AT]CC
1184 GGACC
BbsI 1279 GAAGAC GAAGAC(2/6)
284 GTCTTC
BmyI 125 GTGCCC G[AGT]GC[ACT]^C
215 GGGCTC
BpuAI 1279 GAAGAC GAAGAC(2/6)
284 GTCTTC
Bsp1286I 125 GTGCCC G[AGT]GC[ACT]^C
215 GGGCTC
Cac8I 180 GCCTGC GC[ACGT]^[ACGT]GC
989 GCCAGC
DraII 1005 GGGACCC [GA]G^G[ACGT]CC[CT]
1453 AGGCCCT
EcoO109I 1005 GGGACCC [GA]G^G[ACGT]CC[CT]
1453 AGGCCCT
HphI 745 GGTGA GGTGA(8/7)
958 GGTGA
MspA1I 233 CAGCTG C[AC]G^C[GT]G
1165 CAGCTG
NspI 440 ACATGT [GA]CATG^[CT]
1205 GCATGT
PvuII 233 CAGCTG CAG^CTG
1165 CAGCTG
SfaNI 271 GATGC GCATC(5/9)
1159 GCATC
#########################################
# 3 OR MORE CUT ENZYMES ( 49 enzymes) #
#########################################
AfaI AluI AlwI BbvI BsaJI
BseRI BsiYI BslI BsmAI BsmFI
BsrI BssKI BstF5I BstNI BstYI
Cfr13I DdeI DpnII EarI EcoRII
Fnu4HI FokI HaeIII HincII HindII
HinfI ItaI Ksp632I MboI MboII
MflI MnlI MseI MvaI MwoI
NdeII NlaIII NlaIV RsaI Sau3AI
Sau96I ScrFI SspI TfiI Tru9I
TseI Tsp509I TspRI XhoII