##################################
# NO CUT ENZYMES (  192 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AciI        AclI        AcyI        AflII       AgeI        
AhdI        Alw44I      AlwNI       AocI        Aor51HI     
ApaI        ApaLI       AscI        AseI        AsnI        
Asp700I     Asp718I     AspEI       AspHI       AspI        
AvaI        AvaII       AviII       AvrII       BamHI       
BanI        BanII       BbeI        BbrPI       BbsI        
BbvCI       BcgI        BciVI       BclI        BcnI        
BfrI        BglI        BlnI        BlpI        BmyI        
BpmI        Bpu1102I    BpuAI       BsaAI       BsaBI       
BsaHI       BsaI        BsaJI       BsaWI       BseAI       
BseRI       BsgI        BsiEI       BsiHKAI     BsiWI       
BsiYI       BslI        BsmAI       BsmBI       BsmI        
BsoBI       Bsp1286I    BspDI       BspEI       BspHI       
BspMI       BsrBI       BsrFI       BssHII      BssSI       
Bst1107I    BstAPI      BstBI       BstEII      BstPI       
BstUI       BstXI       BstZ17I     Bsu36I      CelII       
CfoI        Cfr10I      ClaI        CpoI        DraII       
DraIII      DrdI        DsaI        EagI        Eam1105I    
EarI        EclXI       Eco47III    Eco52I      Eco81I      
EcoNI       EcoO109I    EcoO65I     EcoRI       EcoRV       
EcoT14I     EcoT22I     FauI        FbaI        FseI        
FspI        HaeII       HapII       HgaI        HhaI        
Hin1I       HinP1I      HincII      HindII      HindIII     
HpaI        HpaII       HphI        KasI        KpnI        
Ksp632I     KspI        MaeII       MamI        MfeI        
MluI        MroI        MspI        MunI        MvnI        
MwoI        NaeI        NarI        NciI        NcoI        
NdeI        NgoMIV      NheI        NlaIV       NotI        
NruI        NsiI        NspV        PacI        PaeR7I      
PflFI       PflMI       PinAI       PleI        PmaCI       
PmeI        PmlI        PpuMI       PshAI       PshBI       
Psp1406I    PstI        PvuI        RcaI        RsrII       
SacI        SacII       SalI        SapI        SbfI        
ScaI        SexAI       SfaNI       SfcI        SfiI        
SfoI        SfuI        SgrAI       SmaI        SnaBI       
SpeI        SphI        Sse8387I    SspI        StuI        
StyI        Tth111I     Van91I      XhoI        XmaCI       
XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   35 enzymes) #
###################################

AflIII        677  ACATGT               A^C[GA][CT]GT
AluI          200  AGCT                 AG^CT
BalI          530  TGGCCA               TGG^CCA
BbvI          331  GCAGC                GCAGC(8/12)
BglII         519  AGATCT               A^GATCT
BsmFI         757  GGGAC                GGGAC(10/14)
Bsp1407I      203  TGTACA               T^GTACA
BspLU11I      677  ACATGT               A^CATGT
BsrDI         534  CATTGC               GCAATG(2/0)
BsrGI         203  TGTACA               T^GTACA
BstYI         519  AGATCT               [GA]^GATC[CT]
Cfr13I        414  GGCCC                G^G[ACGT]CC
DraI          568  TTTAAA               TTT^AAA
EaeI          530  TGGCCA               [CT]^GGCC[GA]
Fnu4HI        331  GCAGC                GC^[ACGT]GC
ItaI          331  GCAGC                GC^[ACGT]GC
MaeIII        392  GTGAC                ^GT[ACGT]AC
MflI          519  AGATCT               [GA]^GATC[CT]
MluNI         530  TGGCCA               TGG^CCA
MscI          530  TGGCCA               TGG^CCA
MslI          297  CATTAGTGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I        199  CAGCTG               C[AC]G^C[GT]G
NspI          677  ACATGT               [GA]CATG^[CT]
PvuII         199  CAGCTG               CAG^CTG
Sau96I        414  GGCCC                G^G[ACGT]CC
SmlI          224  CTCAAG               C^T[CT][GA]AG
SspBI         203  TGTACA               T^GTACA
SwaI          567  ATTTAAAT             ATTT^AAAT
TaqI          284  TCGA                 T^CGA
TseI          331  GCAGC                G^C[AT]GC
Tsp45I        392  GTGAC                ^GT[GC]AC
TthHB8I       284  TCGA                 T^CGA
XbaI          522  TCTAGA               T^CTAGA
XcmI          625  CCAAAAGACACTTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII         519  AGATCT               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   11 enzymes) #
###################################

AlwI          286  GATCC                GGATC(4/5)
              172  GGATC               
BfaI           62  CTAG                 C^TAG
              523  CTAG                
BstF5I        369  GGATG                GGATG(2/0)
              388  GGATG               
Cac8I         292  GCCAGC               GC[ACGT]^[ACGT]GC
              739  GCACGC              
DdeI          549  CTTAG                C^T[ACGT]AG
              576  CTTAG               
FokI          369  GGATG                GGATG(9/13)
              388  GGATG               
HinfI         498  GAATC                G^A[ACGT]TC
              639  GAATC               
MaeI           62  CTAG                 C^TAG
              523  CTAG                
MnlI          223  CCTC                 CCTC(7/6)
              745  CCTC                
TfiI          498  GAATC                G^A[AT]TC
              639  GAATC               
TspRI         340  CAGTG                CA[GC]TG[ACGT][ACGT]^
              773  CAGTG               


#########################################
# 3 OR MORE CUT ENZYMES (   20 enzymes) #
#########################################

AcsI        AfaI        ApoI        BsrI        BssKI       
BstNI       DpnII       EcoRII      HaeIII      MboI        
MboII       MseI        MvaI        NdeII       NlaIII      
RsaI        Sau3AI      ScrFI       Tru9I       Tsp509I