Restriction map for
pF1KB8554
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# NO CUT ENZYMES ( 104 enzymes) #
##################################
AatII Acc65I AccIII AclI AflII
AgeI AhdI Alw44I AlwNI AocI
Aor51HI ApaI ApaLI AscI Asp700I
Asp718I AspEI AspI AviII BalI
BbeI BbvCI BcgI BfrI BglI
BlpI Bpu1102I BsaWI BseAI BsiEI
BsiWI BsmBI BspEI BspHI BspLU11I
BsrFI BssHII BssSI BstBI BstEII
BstPI Bsu36I CelII Cfr10I CpoI
DraI DraIII DrdI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO65I FseI FspI HincII HindII
HindIII HpaI KasI KpnI KspI
MluI MluNI MroI MscI NaeI
NarI NgoMIV NheI NotI NruI
NspV PacI PflFI PinAI PmeI
Psp1406I PvuI RcaI RsrII SacI
SacII SalI SapI SbfI ScaI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI Sse8387I SwaI Tth111I
XbaI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 46 enzymes) #
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AccI 2361 GTATAC GT^[AC][GT]AC
AccII 1855 CGCG CG^CG
AflIII 311 ACACGT A^C[GA][CT]GT
AseI 366 ATTAAT AT^TAAT
AsnI 366 ATTAAT AT^TAAT
AvrII 1962 CCTAGG C^CTAGG
BanII 1511 GGGCTC G[GA]GC[CT]^C
BclI 2090 TGATCA T^GATCA
BcnI 1533 CCCGG CC^[GC]GG
BlnI 1962 CCTAGG C^CTAGG
BsaBI 2430 GATGTACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsmI 1737 GCATTC GAATGC(1/-1)
Bsp1407I 2432 TGTACA T^GTACA
BspDI 1025 ATCGAT AT^CGAT
BspMI 2368 ACCTGC ACCTGC(4/8)
BsrDI 738 CATTGC GCAATG(2/0)
BsrGI 2432 TGTACA T^GTACA
Bst1107I 2361 GTATAC GTA^TAC
BstUI 1855 CGCG CG^CG
BstZ17I 2361 GTATAC GTA^TAC
CfoI 2056 GCGC GCG^C
ClaI 1025 ATCGAT AT^CGAT
EaeI 1464 CGGCCA [CT]^GGCC[GA]
EarI 151 CTCTTC CTCTTC(1/4)
EcoRI 1081 GAATTC G^AATTC
EcoRV 1414 GATATC GAT^ATC
EcoT22I 527 ATGCAT ATGCA^T
FauI 2711 GCGGG CCCGC(4/6)
FbaI 2090 TGATCA T^GATCA
HaeII 2055 AGCGCC [GA]GCGC^[CT]
HapII 1534 CCGG C^CGG
HhaI 2056 GCGC GCG^C
HinP1I 2056 GCGC G^CGC
HpaII 1534 CCGG C^CGG
Ksp632I 151 CTCTTC CTCTTC(1/4)
MamI 2430 GATGTACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MspI 1534 CCGG C^CGG
MvnI 1855 CGCG CG^CG
NciI 1533 CCCGG CC^[GC]GG
NdeI 1938 CATATG CA^TATG
NsiI 527 ATGCAT ATGCA^T
PshAI 1809 GACCTTTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 366 ATTAAT AT^TAAT
SphI 2492 GCATGC GCATG^C
SspBI 2432 TGTACA T^GTACA
StuI 698 AGGCCT AGG^CCT
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AcyI 445 GGCGTC G[GA]^CG[CT]C
1536 GGCGTC
BanI 183 GGTGCC G^G[CT][GA]CC
1755 GGTGCC
BbrPI 312 CACGTG CAC^GTG
761 CACGTG
BbsI 1498 GAAGAC GAAGAC(2/6)
1957 GTCTTC
BglII 710 AGATCT A^GATCT
1263 AGATCT
BpuAI 1498 GAAGAC GAAGAC(2/6)
1957 GTCTTC
BsaHI 445 GGCGTC G[GA]^CG[CT]C
1536 GGCGTC
BsaI 1680 GAGACC GGTCTC(1/5)
148 GGTCTC
BsgI 1246 CTGCAC GTGCAG(16/14)
358 GTGCAG
BsrBI 687 GAGCGG CCGCTC(-3/-3)
2709 GAGCGG
BstAPI 1248 GCACAATATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1697 GCACAGGCTGC
BstXI 116 CCATACGGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2115 CCAGCTCCTTGG
DraII 263 AGGACCC [GA]G^G[ACGT]CC[CT]
1592 GGGTCCC
DsaI 614 CCATGG C^C[GA][CT]GG
1232 CCATGG
EcoO109I 263 AGGACCC [GA]G^G[ACGT]CC[CT]
1592 GGGTCCC
Hin1I 445 GGCGTC G[GA]^CG[CT]C
1536 GGCGTC
MfeI 245 CAATTG C^AATTG
1010 CAATTG
MunI 245 CAATTG C^AATTG
1010 CAATTG
NcoI 614 CCATGG C^CATGG
1232 CCATGG
PflMI 609 CCAAGCCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
769 CCAAGACCTGG
PleI 595 GACTC GAGTC(4/5)
2661 GAGTC
PmaCI 312 CACGTG CAC^GTG
761 CACGTG
PmlI 312 CACGTG CAC^GTG
761 CACGTG
PpuMI 263 AGGACCC [GA]G^G[AT]CC[CT]
1592 GGGTCCC
PstI 458 CTGCAG CTGCA^G
1545 CTGCAG
PvuII 2083 CAGCTG CAG^CTG
2582 CAGCTG
SexAI 559 ACCAGGT A^CC[AT]GGT
1637 ACCAGGT
SspI 1773 AATATT AAT^ATT
2025 AATATT
Van91I 609 CCAAGCCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
769 CCAAGACCTGG
XcmI 281 CCAACATCACTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1718 CCATCATCAAATTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 78 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI AspHI AvaI AvaII BamHI
BbvI BciVI BfaI BmyI BpmI
BsaAI BsaJI BseRI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BsrI BssKI BstF5I BstNI BstYI
Cac8I Cfr13I DdeI DpnII EcoRII
EcoT14I Fnu4HI FokI HaeIII HgaI
HinfI HphI ItaI MaeI MaeII
MaeIII MboI MboII MflI MnlI
MseI MslI MspA1I MvaI MwoI
NdeII NlaIII NlaIV NspI PaeR7I
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XhoI XhoII