Restriction map for
pF1KB8562
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# NO CUT ENZYMES ( 90 enzymes) #
##################################
AatII AccI AccIII AclI AflII
AgeI Alw44I Aor51HI ApaLI AscI
AseI AsnI AspI AvrII BbrPI
BfrI BlnI BsaAI BsaWI BseAI
BsiEI BsiWI BsmBI Bsp1407I BspEI
BspHI BspLU11I BsrDI BsrGI BssSI
Bst1107I BstEII BstPI BstZ17I CpoI
DraI DraIII DsaI EagI EclXI
Eco47III Eco52I EcoNI EcoO65I FseI
HincII HindII HindIII HpaI KspI
MfeI MluI MroI MunI NcoI
NdeI NheI NotI NruI PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
PvuII RcaI RsrII SacI SacII
SalI SbfI ScaI SexAI SgrAI
SmlI SnaBI SpeI Sse8387I SspBI
SspI StuI SwaI Tth111I XhoI
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# ONE CUT ENZYMES ( 41 enzymes) #
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AcsI 644 GAATTC [GA]^AATT[CT]
AcyI 1160 GGCGCC G[GA]^CG[CT]C
AflIII 1667 ACGTGT A^C[GA][CT]GT
ApoI 644 GAATTC [GA]^AATT[CT]
Asp700I 652 GAAAAGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 1726 GTGCTC G[AT]GC[AT]^C
AviII 1763 TGCGCA TGC^GCA
BamHI 546 GGATCC G^GATCC
BbeI 1160 GGCGCC GGCGC^C
BbvCI 1928 CCTCAGC CCTCAGC(-5/-2)
BcgI 1343 GCATTCTCTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 1875 GGATAC GTATCC(6/5)
BsaBI 1620 GATCAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 1160 GGCGCC G[GA]^CG[CT]C
BsiHKAI 1726 GTGCTC G[AT]GC[AT]^C
BspDI 826 ATCGAT AT^CGAT
BsrBI 2623 CCGCTC CCGCTC(-3/-3)
BssHII 235 GCGCGC G^CGCGC
BstBI 1028 TTCGAA TT^CGAA
BstXI 2318 CCATCCTCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
ClaI 826 ATCGAT AT^CGAT
DrdI 1819 GACTTCGGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRI 644 GAATTC G^AATTC
EcoRV 1153 GATATC GAT^ATC
EcoT22I 1196 ATGCAT ATGCA^T
FspI 1763 TGCGCA TGC^GCA
Hin1I 1160 GGCGCC G[GA]^CG[CT]C
KasI 1160 GGCGCC G^GCGCC
MamI 1620 GATCAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NarI 1160 GGCGCC GG^CGCC
NsiI 1196 ATGCAT ATGCA^T
NspI 2495 GCATGC [GA]CATG^[CT]
NspV 1028 TTCGAA TT^CGAA
SfiI 2562 GGCCTCCCTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 1160 GGCGCC GGC^GCC
SfuI 1028 TTCGAA TT^CGAA
SphI 2495 GCATGC GCATG^C
Tsp509I 645 AATT ^AATT
XbaI 1449 TCTAGA T^CTAGA
XcmI 1838 CCATCCTGATCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 652 GAAAAGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 33 enzymes) #
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Acc65I 157 GGTACC G^GTACC
1738 GGTACC
AhdI 1440 GACCAACGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2288 GACTCCTGGTC
Asp718I 157 GGTACC G^GTACC
1738 GGTACC
AspEI 1440 GACCAACGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2288 GACTCCTGGTC
BalI 392 TGGCCA TGG^CCA
2720 TGGCCA
BclI 1619 TGATCA T^GATCA
1844 TGATCA
BglII 1565 AGATCT A^GATCT
1625 AGATCT
BlpI 1366 GCTCAGC GC^T[ACGT]AGC
2397 GCTCAGC
Bpu1102I 1366 GCTCAGC GC^T[ACGT]AGC
2397 GCTCAGC
BsaI 373 GAGACC GGTCTC(1/5)
1438 GAGACC
BsmI 1198 GCATTC GAATGC(1/-1)
1343 GCATTC
BspMI 365 ACCTGC ACCTGC(4/8)
2114 ACCTGC
BstAPI 714 GCAGCCGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
987 GCAGGCACTGC
CelII 1366 GCTCAGC GC^T[ACGT]AGC
2397 GCTCAGC
Eam1105I 1440 GACCAACGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2288 GACTCCTGGTC
EcoT14I 344 CCAAGG C^C[AT][AT]GG
2672 CCAAGG
FbaI 1619 TGATCA T^GATCA
1844 TGATCA
HaeII 1034 GGCGCT [GA]GCGC^[CT]
1160 GGCGCC
HgaI 1803 GCGTC GACGC(5/10)
2612 GCGTC
KpnI 157 GGTACC GGTAC^C
1738 GGTACC
MluNI 392 TGGCCA TGG^CCA
2720 TGGCCA
MscI 392 TGGCCA TGG^CCA
2720 TGGCCA
MseI 476 TTAA T^TAA
1090 TTAA
NaeI 99 GCCGGC GCC^GGC
2559 GCCGGC
NgoMIV 99 GCCGGC G^CCGGC
2559 GCCGGC
PflMI 1209 CCAGGTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1950 CCACCCACTGG
SapI 594 GCTCTTC GCTCTTC(1/4)
1131 GCTCTTC
SmaI 153 CCCGGG CCC^GGG
1934 CCCGGG
StyI 344 CCAAGG C^C[AT][AT]GG
2672 CCAAGG
Tru9I 476 TTAA T^TAA
1090 TTAA
Van91I 1209 CCAGGTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1950 CCACCCACTGG
XmaCI 153 CCCGGG C^CCGGG
1934 CCCGGG
XmaI 153 CCCGGG C^CCGGG
1934 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 94 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AlwNI AocI ApaI AvaI AvaII
BanI BanII BbsI BbvI BcnI
BfaI BglI BmyI BpmI BpuAI
BsaJI BseRI BsgI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BsrFI
BsrI BssKI BstF5I BstNI BstUI
BstYI Bsu36I Cac8I CfoI Cfr10I
Cfr13I DdeI DpnII DraII EaeI
EarI Eco81I EcoO109I EcoRII FauI
Fnu4HI FokI HaeIII HapII HhaI
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MnlI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV PleI PpuMI
PstI RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI TaqI TfiI TseI
Tsp45I TspRI TthHB8I XhoII