Restriction map for
pF1KB0411
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# NO CUT ENZYMES ( 121 enzymes) #
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AatII AccII AccIII AclI AflII
AflIII AgeI ApaI AscI Asp700I
AspI AviII AvrII BamHI BbrPI
BbvCI BcgI BciVI BclI BcnI
BfrI BglI BglII BlnI BsaAI
BsaBI BsaI BsaWI BseAI BseRI
BsiEI BsiWI BsmBI BsmI BspDI
BspEI BspLU11I BspMI BsrBI BsrFI
BssHII BstAPI BstBI BstEII BstPI
BstUI BstXI BstYI Cfr10I ClaI
CpoI DraIII DrdI EagI EclXI
Eco52I EcoNI EcoO65I EcoRI EcoRV
EcoT22I FbaI FseI FspI HgaI
HincII HindII HpaI KspI MamI
MfeI MflI MluI MroI MspA1I
MunI MvnI NaeI NciI NdeI
NgoMIV NheI NotI NruI NsiI
NspV PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI Psp1406I PvuI
PvuII RsrII SacI SacII SalI
SbfI ScaI SexAI SfiI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I StuI Tth111I Van91I XbaI
XcmI XhoI XhoII XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 70 enzymes) #
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Acc65I 207 GGTACC G^GTACC
AccI 454 GTATAC GT^[AC][GT]AC
AcyI 439 GGCGCC G[GA]^CG[CT]C
AhdI 141 GACCATGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 1281 GTGCAC G^TGCAC
AlwNI 1195 CAGAGTCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 850 CCTAAGG CC^T[ACGT]AGG
Aor51HI 986 AGCGCT AGC^GCT
ApaLI 1281 GTGCAC G^TGCAC
AseI 48 ATTAAT AT^TAAT
AsnI 48 ATTAAT AT^TAAT
Asp718I 207 GGTACC G^GTACC
AspEI 141 GACCATGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI 434 CTCGGG C^[CT]CG[GA]G
BalI 198 TGGCCA TGG^CCA
BanII 882 GAGCCC G[GA]GC[CT]^C
BbeI 439 GGCGCC GGCGC^C
BbsI 1051 GAAGAC GAAGAC(2/6)
BfaI 767 CTAG C^TAG
BlpI 998 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 998 GCTCAGC GC^T[ACGT]AGC
BpuAI 1051 GAAGAC GAAGAC(2/6)
BsaHI 439 GGCGCC G[GA]^CG[CT]C
BsgI 900 GTGCAG GTGCAG(16/14)
BsmAI 183 GTCTC GTCTC(1/5)
BsoBI 434 CTCGGG C^[CT]CG[GA]G
Bsp1407I 1019 TGTACA T^GTACA
BspHI 753 TCATGA T^CATGA
BsrGI 1019 TGTACA T^GTACA
BssSI 559 CACGAG CACGAG(-5/-1)
Bst1107I 454 GTATAC GTA^TAC
BstZ17I 454 GTATAC GTA^TAC
Bsu36I 850 CCTAAGG CC^T[ACGT]AGG
CelII 998 GCTCAGC GC^T[ACGT]AGC
DraI 60 TTTAAA TTT^AAA
DsaI 211 CCATGG C^C[GA][CT]GG
EaeI 198 TGGCCA [CT]^GGCC[GA]
Eam1105I 141 GACCATGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 986 AGCGCT AGC^GCT
Eco81I 850 CCTAAGG CC^T[ACGT]AGG
EcoT14I 211 CCATGG C^C[AT][AT]GG
FauI 1167 CCCGC CCCGC(4/6)
HapII 555 CCGG C^CGG
Hin1I 439 GGCGCC G[GA]^CG[CT]C
HindIII 1097 AAGCTT A^AGCTT
HpaII 555 CCGG C^CGG
KasI 439 GGCGCC G^GCGCC
KpnI 207 GGTACC GGTAC^C
MaeI 767 CTAG C^TAG
MaeII 357 ACGT A^CGT
MluNI 198 TGGCCA TGG^CCA
MscI 198 TGGCCA TGG^CCA
MspI 555 CCGG C^CGG
NarI 439 GGCGCC GG^CGCC
NcoI 211 CCATGG C^CATGG
PacI 49 TTAATTAA TTAAT^TAA
PpuMI 854 AGGACCT [GA]G^G[AT]CC[CT]
PshAI 1066 GACTGCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 48 ATTAAT AT^TAAT
PstI 1068 CTGCAG CTGCA^G
RcaI 753 TCATGA T^CATGA
SapI 109 GAAGAGC GCTCTTC(1/4)
SfaNI 1308 GATGC GCATC(5/9)
SfoI 439 GGCGCC GGC^GCC
SspBI 1019 TGTACA T^GTACA
SspI 412 AATATT AAT^ATT
StyI 211 CCATGG C^C[AT][AT]GG
SwaI 59 ATTTAAAT ATTT^AAAT
TaqI 1337 TCGA T^CGA
TthHB8I 1337 TCGA T^CGA
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# TWO CUT ENZYMES ( 17 enzymes) #
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AcsI 320 GAATTT [GA]^AATT[CT]
679 GAATTT
ApoI 320 GAATTT [GA]^AATT[CT]
679 GAATTT
AspHI 12 GAGCAC G[AT]GC[AT]^C
1281 GTGCAC
BbvI 189 GCTGC GCAGC(8/12)
1230 GCTGC
BsiHKAI 12 GAGCAC G[AT]GC[AT]^C
1281 GTGCAC
BsmFI 717 GGGAC GGGAC(10/14)
1377 GGGAC
BsrDI 1056 CATTGC GCAATG(2/0)
1266 CATTGC
DraII 854 AGGACCT [GA]G^G[ACGT]CC[CT]
1157 AGGCCCT
EarI 34 GAAGAG CTCTTC(1/4)
109 GAAGAG
EcoO109I 854 AGGACCT [GA]G^G[ACGT]CC[CT]
1157 AGGCCCT
Ksp632I 34 GAAGAG CTCTTC(1/4)
109 GAAGAG
NspI 705 GCATGT [GA]CATG^[CT]
956 ACATGC
PleI 1204 GACTC GAGTC(4/5)
1197 GAGTC
SfcI 930 CTACAG C^T[GA][CT]AG
1068 CTGCAG
SmlI 712 CTCAAG C^T[CT][GA]AG
1312 CTCAAG
TseI 189 GCTGC G^C[AT]GC
1230 GCTGC
Tsp45I 23 GTGAC ^GT[GC]AC
697 GTGAC
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# 3 OR MORE CUT ENZYMES ( 50 enzymes) #
#########################################
AciI AfaI AluI AlwI AvaII
BanI BmyI BpmI BsaJI BsiYI
BslI Bsp1286I BsrI BssKI BstF5I
BstNI Cac8I CfoI Cfr13I DdeI
DpnII EcoRII Fnu4HI FokI HaeII
HaeIII HhaI HinP1I HinfI HphI
ItaI MaeIII MboI MboII MnlI
MseI MslI MvaI MwoI NdeII
NlaIII NlaIV RsaI Sau3AI Sau96I
ScrFI TfiI Tru9I Tsp509I TspRI