Restriction map for
pF1KB0415
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# NO CUT ENZYMES ( 105 enzymes) #
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AatII Acc65I AccI AclI AcsI
AflII AflIII AhdI Aor51HI ApaI
ApoI AscI AseI AsnI Asp718I
AspEI AspI AvrII BamHI BanI
BbeI BciVI BclI BfaI BfrI
BglII BlnI BlpI Bpu1102I BsaBI
BsaI BsiWI BsmBI BsmI Bsp1407I
BspDI BspHI BspLU11I BsrGI BssSI
Bst1107I BstBI BstEII BstPI BstYI
BstZ17I CelII ClaI CpoI DraI
DrdI Eam1105I Eco47III EcoNI EcoO65I
EcoRI FbaI HincII HindII HindIII
HpaI KasI KpnI KspI MaeI
MamI MfeI MflI MluI MseI
MunI NarI NdeI NheI NotI
NruI NspV PacI PflFI PmeI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SacII SalI SbfI ScaI
SexAI SfiI SfoI SfuI SnaBI
SpeI Sse8387I SspBI SwaI Tru9I
Tsp509I Tth111I XbaI XcmI XhoII
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# ONE CUT ENZYMES ( 57 enzymes) #
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AccIII 365 TCCGGA T^CCGGA
AcyI 841 GACGCC G[GA]^CG[CT]C
AgeI 1290 ACCGGT A^CCGGT
Alw44I 316 GTGCAC G^TGCAC
AlwI 649 GATCC GGATC(4/5)
AlwNI 668 CAGTCACTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 855 CCTGAGG CC^T[ACGT]AGG
ApaLI 316 GTGCAC G^TGCAC
Asp700I 275 GAAACTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AviII 78 TGCGCA TGC^GCA
BalI 680 TGGCCA TGG^CCA
BbrPI 862 CACGTG CAC^GTG
BcgI 92 CGAGAGGCGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglI 31 GCCCTGCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaAI 862 CACGTG [CT]AC^GT[GA]
BsaHI 841 GACGCC G[GA]^CG[CT]C
BseAI 365 TCCGGA T^CCGGA
BspEI 365 TCCGGA T^CCGGA
BspMI 136 ACCTGC ACCTGC(4/8)
BssHII 17 GCGCGC G^CGCGC
BstAPI 861 GCACGTGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Bsu36I 855 CCTGAGG CC^T[ACGT]AGG
DpnII 649 GATC ^GATC
Eco81I 855 CCTGAGG CC^T[ACGT]AGG
EcoRV 767 GATATC GAT^ATC
EcoT22I 1210 ATGCAT ATGCA^T
FseI 294 GGCCGGCC GGCCGG^CC
FspI 78 TGCGCA TGC^GCA
Hin1I 841 GACGCC G[GA]^CG[CT]C
MboI 649 GATC ^GATC
MluNI 680 TGGCCA TGG^CCA
MroI 365 TCCGGA T^CCGGA
MscI 680 TGGCCA TGG^CCA
NcoI 929 CCATGG C^CATGG
NdeII 649 GATC ^GATC
NsiI 1210 ATGCAT ATGCA^T
NspI 1208 GCATGC [GA]CATG^[CT]
PaeR7I 1326 CTCGAG C^TCGAG
PflMI 212 CCAGCAGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 1290 ACCGGT A^CCGGT
PmaCI 862 CACGTG CAC^GTG
PmlI 862 CACGTG CAC^GTG
PstI 197 CTGCAG CTGCA^G
SacI 760 GAGCTC GAGCT^C
SapI 369 GAAGAGC GCTCTTC(1/4)
Sau3AI 649 GATC ^GATC
SgrAI 1289 CACCGGTG C[GA]^CCGG[CT]G
SmaI 141 CCCGGG CCC^GGG
SmlI 1326 CTCGAG C^T[CT][GA]AG
SphI 1208 GCATGC GCATG^C
SspI 1024 AATATT AAT^ATT
StuI 1249 AGGCCT AGG^CCT
Van91I 212 CCAGCAGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 1326 CTCGAG C^TCGAG
XmaCI 141 CCCGGG C^CCGGG
XmaI 141 CCCGGG C^CCGGG
XmnI 275 GAAACTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 28 enzymes) #
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AccII 18 CGCG CG^CG
42 CGCG
AfaI 1084 GTAC GT^AC
1221 GTAC
BbvCI 378 CCTCAGC CCTCAGC(-5/-2)
246 GCTGAGG
BsaWI 365 TCCGGA [AT]^CCGG[AT]
1290 ACCGGT
BsgI 256 GTGCAG GTGCAG(16/14)
868 GTGCAG
BsiEI 297 CGGCCG CG[GA][CT]^CG
470 CGGCCG
BsmAI 119 GAGAC GTCTC(1/5)
548 GAGAC
BsrBI 50 GAGCGG CCGCTC(-3/-3)
849 GAGCGG
BsrDI 1143 CATTGC GCAATG(2/0)
383 GCAATG
BstUI 18 CGCG CG^CG
42 CGCG
DraIII 175 CACTGGGTG CAC[ACGT][ACGT][ACGT]^GTG
862 CACGTGGTG
EagI 297 CGGCCG C^GGCCG
470 CGGCCG
EarI 1030 CTCTTC CTCTTC(1/4)
369 GAAGAG
EclXI 297 CGGCCG C^GGCCG
470 CGGCCG
Eco52I 297 CGGCCG C^GGCCG
470 CGGCCG
FauI 692 CCCGC CCCGC(4/6)
1182 GCGGG
HaeII 54 GGCGCT [GA]GCGC^[CT]
68 GGCGCT
HgaI 841 GACGC GACGC(5/10)
578 GCGTC
HphI 447 GGTGA GGTGA(8/7)
707 TCACC
Ksp632I 1030 CTCTTC CTCTTC(1/4)
369 GAAGAG
MaeII 863 ACGT A^CGT
1171 ACGT
MvnI 18 CGCG CG^CG
42 CGCG
NaeI 192 GCCGGC GCC^GGC
295 GCCGGC
NgoMIV 192 GCCGGC G^CCGGC
295 GCCGGC
RsaI 1084 GTAC GT^AC
1221 GTAC
SfcI 197 CTGCAG C^T[GA][CT]AG
744 CTACAG
TaqI 1254 TCGA T^CGA
1327 TCGA
TthHB8I 1254 TCGA T^CGA
1327 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 68 enzymes) #
#########################################
AciI AluI AspHI AvaI AvaII
BanII BbsI BbvI BcnI BmyI
BpmI BpuAI BsaJI BseRI BsiHKAI
BsiYI BslI BsmFI BsoBI Bsp1286I
BsrFI BsrI BssKI BstF5I BstNI
BstXI Cac8I CfoI Cfr10I Cfr13I
DdeI DraII DsaI EaeI EcoO109I
EcoRII EcoT14I Fnu4HI FokI HaeIII
HapII HhaI HinP1I HinfI HpaII
ItaI MaeIII MboII MnlI MslI
MspA1I MspI MvaI MwoI NciI
NlaIII NlaIV PleI PpuMI PvuII
Sau96I ScrFI SfaNI StyI TfiI
TseI Tsp45I TspRI