Restriction map for
pF1KB0423
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# NO CUT ENZYMES ( 140 enzymes) #
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AatII Acc65I AccII AccIII AclI
AcyI AflII AflIII AgeI AhdI
Alw44I AocI Aor51HI ApaI ApaLI
AscI AseI AsnI Asp718I AspEI
AspI AviII AvrII BalI BbeI
BbrPI BbvCI BcgI BciVI BclI
BfrI BglI BglII BlnI BlpI
Bpu1102I BsaAI BsaBI BsaHI BsaI
BseAI BsiEI BsiWI BsmBI Bsp1407I
BspDI BspEI BspLU11I BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI
Bst1107I BstAPI BstEII BstPI BstUI
BstZ17I Bsu36I CelII Cfr10I ClaI
CpoI DraI DraII DraIII EaeI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoO109I EcoO65I EcoRI EcoRV
FbaI FseI FspI HaeII HaeIII
HgaI Hin1I HindIII HpaI KasI
KpnI KspI MamI MluI MluNI
MroI MscI MvnI NaeI NarI
NcoI NgoMIV NheI NotI NruI
NspI PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PvuI RsrII SacII
SbfI ScaI SfiI SfoI SgrAI
SmaI SmlI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI SwaI
Tth111I XcmI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 48 enzymes) #
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AccI 134 GTCGAC GT^[AC][GT]AC
AcsI 33 AAATTT [GA]^AATT[CT]
AlwNI 491 CAGAAGCTG CAG[ACGT][ACGT][ACGT]^CTG
ApoI 33 AAATTT [GA]^AATT[CT]
Asp700I 1296 GAATGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI 299 CCCGAG C^[CT]CG[GA]G
AvaII 169 GGTCC G^G[AT]CC
BanI 197 GGCACC G^G[CT][GA]CC
BanII 1240 GAGCTC G[GA]GC[CT]^C
BsaWI 995 ACCGGA [AT]^CCGG[AT]
BsgI 860 GTGCAG GTGCAG(16/14)
BsmI 637 GCATTC GAATGC(1/-1)
BsoBI 299 CCCGAG C^[CT]CG[GA]G
BspHI 306 TCATGA T^CATGA
BstBI 76 TTCGAA TT^CGAA
Cac8I 496 GCTGGC GC[ACGT]^[ACGT]GC
CfoI 4 GCGC GCG^C
Cfr13I 169 GGTCC G^G[ACGT]CC
DrdI 232 GACTCTGGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 686 CCGTGG C^C[GA][CT]GG
EcoNI 1227 CCTCACAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT14I 1336 CCTTGG C^C[AT][AT]GG
EcoT22I 749 ATGCAT ATGCA^T
HhaI 4 GCGC GCG^C
HinP1I 4 GCGC G^CGC
MaeII 324 ACGT A^CGT
MfeI 908 CAATTG C^AATTG
MseI 161 TTAA T^TAA
MspA1I 1270 CAGCTG C[AC]G^C[GT]G
MunI 908 CAATTG C^AATTG
NdeI 254 CATATG CA^TATG
NsiI 749 ATGCAT ATGCA^T
NspV 76 TTCGAA TT^CGAA
PflMI 490 CCAGAAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PstI 182 CTGCAG CTGCA^G
PvuII 1270 CAGCTG CAG^CTG
RcaI 306 TCATGA T^CATGA
SacI 1240 GAGCTC GAGCT^C
SalI 134 GTCGAC G^TCGAC
SapI 293 GCTCTTC GCTCTTC(1/4)
Sau96I 169 GGTCC G^G[ACGT]CC
SexAI 710 ACCTGGT A^CC[AT]GGT
SfuI 76 TTCGAA TT^CGAA
StyI 1336 CCTTGG C^C[AT][AT]GG
Tru9I 161 TTAA T^TAA
Van91I 490 CCAGAAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 1152 TCTAGA T^CTAGA
XmnI 1296 GAATGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 16 enzymes) #
###################################
AfaI 727 GTAC GT^AC
847 GTAC
BamHI 612 GGATCC G^GATCC
784 GGATCC
BbsI 436 GAAGAC GAAGAC(2/6)
1260 GAAGAC
BcnI 377 CCCGG CC^[GC]GG
801 CCGGG
BpmI 891 CTCCAG CTGGAG(16/14)
539 CTGGAG
BpuAI 436 GAAGAC GAAGAC(2/6)
1260 GAAGAC
FauI 555 CCCGC CCCGC(4/6)
944 CCCGC
HincII 134 GTCGAC GT[CT]^[GA]AC
939 GTTGAC
HindII 134 GTCGAC GT[CT]^[GA]AC
939 GTTGAC
NciI 377 CCCGG CC^[GC]GG
801 CCGGG
RsaI 727 GTAC GT^AC
847 GTAC
SfcI 182 CTGCAG C^T[GA][CT]AG
1118 CTGTAG
TaqI 77 TCGA T^CGA
135 TCGA
Tsp45I 60 GTCAC ^GT[GC]AC
1028 GTGAC
TspRI 842 CAGTG CA[GC]TG[ACGT][ACGT]^
1392 CAGTG
TthHB8I 77 TCGA T^CGA
135 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 54 enzymes) #
#########################################
AciI AluI AlwI AspHI BbvI
BfaI BmyI BsaJI BseRI BsiHKAI
BsiYI BslI BsmAI BsmFI Bsp1286I
BsrI BssKI BstF5I BstNI BstXI
BstYI DdeI DpnII EarI EcoRII
Fnu4HI FokI HapII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeIII
MboI MboII MflI MnlI MslI
MspI MvaI MwoI NdeII NlaIII
NlaIV PleI Sau3AI ScrFI SfaNI
TfiI TseI Tsp509I XhoII