Restriction map for
pF1KB0428
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# NO CUT ENZYMES ( 102 enzymes) #
##################################
AatII Acc65I AccI AclI AcyI
AflIII AgeI AhdI Aor51HI ApaI
AscI AseI AsnI Asp718I AspEI
AvaI AvrII BbeI BbrPI BcgI
BclI BlnI BsaBI BsaHI BsiEI
BsiWI BsmBI BsmI BsoBI BspDI
BspLU11I BsrFI BssHII Bst1107I BstBI
BstEII BstPI BstZ17I Cfr10I ClaI
CpoI DrdI EagI Eam1105I EclXI
Eco47III Eco52I EcoNI EcoO65I EcoRI
EcoRV FbaI FseI HaeII HgaI
Hin1I HpaI KasI KpnI MamI
MfeI MluI MunI NaeI NarI
NdeI NgoMIV NheI NotI NruI
NspV PacI PaeR7I PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PstI PvuI RsrII SalI SbfI
ScaI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI Sse8387I SspI
StuI SwaI TaqI TthHB8I XhoI
XmaCI XmaI
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# ONE CUT ENZYMES ( 46 enzymes) #
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AccII 95 CGCG CG^CG
AflII 1902 CTTAAG C^TTAAG
AocI 1859 CCTCAGG CC^T[ACGT]AGG
AspI 2538 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 152 TGCGCA TGC^GCA
BamHI 578 GGATCC G^GATCC
BanI 1437 GGCACC G^G[CT][GA]CC
BbvCI 1576 GCTGAGG CCTCAGC(-5/-2)
BfrI 1902 CTTAAG C^TTAAG
BglI 1052 GCCTGGTAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 847 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 847 GCTGAGC GC^T[ACGT]AGC
BsaAI 2414 TACGTG [CT]AC^GT[GA]
BsgI 1193 CTGCAC GTGCAG(16/14)
Bsp1407I 1224 TGTACA T^GTACA
BspHI 2460 TCATGA T^CATGA
BsrGI 1224 TGTACA T^GTACA
BssSI 274 CTCGTG CACGAG(-5/-1)
BstAPI 339 GCAGGAAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 95 CGCG CG^CG
Bsu36I 1859 CCTCAGG CC^T[ACGT]AGG
CelII 847 GCTGAGC GC^T[ACGT]AGC
DraI 1393 TTTAAA TTT^AAA
DraIII 2014 CACCAGGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco81I 1859 CCTCAGG CC^T[ACGT]AGG
EcoT22I 1999 ATGCAT ATGCA^T
FauI 96 GCGGG CCCGC(4/6)
FspI 152 TGCGCA TGC^GCA
HincII 1919 GTCAAC GT[CT]^[GA]AC
HindII 1919 GTCAAC GT[CT]^[GA]AC
KspI 94 CCGCGG CCGC^GG
MaeII 2415 ACGT A^CGT
MvnI 95 CGCG CG^CG
NcoI 574 CCATGG C^CATGG
NsiI 1999 ATGCAT ATGCA^T
NspI 648 GCATGC [GA]CATG^[CT]
PflFI 2538 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC
RcaI 2460 TCATGA T^CATGA
SacI 2235 GAGCTC GAGCT^C
SacII 94 CCGCGG CCGC^GG
SexAI 2015 ACCAGGT A^CC[AT]GGT
SfcI 1830 CTGTAG C^T[GA][CT]AG
SphI 648 GCATGC GCATG^C
SspBI 1224 TGTACA T^GTACA
Tth111I 2538 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 1161 TCTAGA T^CTAGA
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# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AccIII 875 TCCGGA T^CCGGA
1295 TCCGGA
AcsI 1787 AAATTC [GA]^AATT[CT]
2157 AAATTT
ApoI 1787 AAATTC [GA]^AATT[CT]
2157 AAATTT
BanII 594 GGGCTC G[GA]GC[CT]^C
2235 GAGCTC
BbsI 302 GAAGAC GAAGAC(2/6)
1841 GAAGAC
BpuAI 302 GAAGAC GAAGAC(2/6)
1841 GAAGAC
BseAI 875 TCCGGA T^CCGGA
1295 TCCGGA
BspEI 875 TCCGGA T^CCGGA
1295 TCCGGA
BspMI 958 ACCTGC ACCTGC(4/8)
730 GCAGGT
BsrDI 1005 CATTGC GCAATG(2/0)
1817 GCAATG
BstF5I 665 CATCC GGATG(2/0)
1470 GGATG
BstXI 828 CCAAGTGTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1317 CCAGCTGTATGG
CfoI 153 GCGC GCG^C
348 GCGC
DraII 1178 AGGACCC [GA]G^G[ACGT]CC[CT]
1366 AGGACCC
EcoO109I 1178 AGGACCC [GA]G^G[ACGT]CC[CT]
1366 AGGACCC
EcoT14I 158 CCAAGG C^C[AT][AT]GG
574 CCATGG
FokI 665 CATCC GGATG(9/13)
1470 GGATG
HhaI 153 GCGC GCG^C
348 GCGC
HinP1I 153 GCGC G^CGC
348 GCGC
MroI 875 TCCGGA T^CCGGA
1295 TCCGGA
PpuMI 1178 AGGACCC [GA]G^G[AT]CC[CT]
1366 AGGACCC
PvuII 1190 CAGCTG CAG^CTG
1318 CAGCTG
SmlI 1902 CTTAAG C^T[CT][GA]AG
2238 CTCAAG
StyI 158 CCAAGG C^C[AT][AT]GG
574 CCATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 86 enzymes) #
#########################################
AciI AfaI AluI Alw44I AlwI
AlwNI ApaLI Asp700I AspHI AvaII
BalI BbvI BciVI BcnI BfaI
BglII BmyI BpmI BsaI BsaJI
BsaWI BseRI BsiHKAI BsiYI BslI
BsmAI BsmFI Bsp1286I BsrBI BsrI
BssKI BstNI BstYI Cac8I Cfr13I
DdeI DpnII DsaI EaeI EarI
EcoRII Fnu4HI HaeIII HapII HindIII
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeIII MboI MboII MflI
MluNI MnlI MscI MseI MslI
MspA1I MspI MvaI MwoI NciI
NdeII NlaIII NlaIV PflMI PleI
RsaI SapI Sau3AI Sau96I ScrFI
SfaNI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI Van91I XcmI XhoII
XmnI