Restriction map for
pF1KB0435
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# NO CUT ENZYMES ( 127 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AflII AgeI AhdI Alw44I AocI
Aor51HI ApaI ApaLI AscI AseI
AsnI Asp700I Asp718I AspEI AspI
AvaII AviII AvrII BamHI BbrPI
BbvCI BciVI BfrI BglII BlnI
BsaBI BsaI BsaWI BseAI BsiEI
BsiWI BsmBI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrFI BsrGI BssHII
BssSI Bst1107I BstBI BstEII BstPI
BstUI BstXI BstZ17I Bsu36I Cfr10I
ClaI CpoI DraI DraIII DrdI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoNI EcoO65I EcoRI EcoRV
EcoT22I FseI FspI HindIII HpaI
KpnI KspI MamI MfeI MluI
MroI MseI MunI MvnI NaeI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspV PacI PflFI
PflMI PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PstI
PvuI RcaI RsrII SacI SacII
SbfI SexAI SfiI SfuI SgrAI
SnaBI SpeI Sse8387I SspBI SspI
StuI SwaI Tru9I Tth111I Van91I
XbaI XmnI
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# ONE CUT ENZYMES ( 50 enzymes) #
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AccI 850 GTCGAC GT^[AC][GT]AC
AcsI 760 GAATTT [GA]^AATT[CT]
AflIII 1004 ACGTGT A^C[GA][CT]GT
AlwI 984 GATCC GGATC(4/5)
AlwNI 832 CAGCTGCTG CAG[ACGT][ACGT][ACGT]^CTG
ApoI 760 GAATTT [GA]^AATT[CT]
AspHI 63 GTGCTC G[AT]GC[AT]^C
BbeI 1121 GGCGCC GGCGC^C
BclI 563 TGATCA T^GATCA
BfaI 1067 CTAG C^TAG
BglI 347 GCCTCCCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaAI 1086 CACGTA [CT]AC^GT[GA]
BsiHKAI 63 GTGCTC G[AT]GC[AT]^C
BsmI 1073 GAATGC GAATGC(1/-1)
BspMI 1317 ACCTGC ACCTGC(4/8)
BstAPI 395 GCATGGTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstYI 983 AGATCC [GA]^GATC[CT]
CfoI 1122 GCGC GCG^C
DraII 722 AGGCCCC [GA]G^G[ACGT]CC[CT]
DsaI 1034 CCACGG C^C[GA][CT]GG
EcoO109I 722 AGGCCCC [GA]G^G[ACGT]CC[CT]
FbaI 563 TGATCA T^GATCA
HaeII 1121 GGCGCC [GA]GCGC^[CT]
HhaI 1122 GCGC GCG^C
HinP1I 1122 GCGC G^CGC
HincII 850 GTCGAC GT[CT]^[GA]AC
HindII 850 GTCGAC GT[CT]^[GA]AC
KasI 1121 GGCGCC G^GCGCC
MaeI 1067 CTAG C^TAG
MaeIII 266 GTCAC ^GT[ACGT]AC
MflI 983 AGATCC [GA]^GATC[CT]
MspA1I 832 CAGCTG C[AC]G^C[GT]G
NarI 1121 GGCGCC GG^CGCC
PaeR7I 1057 CTCGAG C^TCGAG
PvuII 832 CAGCTG CAG^CTG
SalI 850 GTCGAC G^TCGAC
SapI 678 GAAGAGC GCTCTTC(1/4)
ScaI 104 AGTACT AGT^ACT
SfcI 1215 CTACAG C^T[GA][CT]AG
SfoI 1121 GGCGCC GGC^GCC
SmaI 672 CCCGGG CCC^GGG
SmlI 1057 CTCGAG C^T[CT][GA]AG
SphI 1181 GCATGC GCATG^C
TfiI 963 GATTC G^A[AT]TC
Tsp45I 266 GTCAC ^GT[GC]AC
Tsp509I 761 AATT ^AATT
XhoI 1057 CTCGAG C^TCGAG
XhoII 983 AGATCC [GA]^GATC[CT]
XmaCI 672 CCCGGG C^CCGGG
XmaI 672 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AfaI 105 GTAC GT^AC
1089 GTAC
BbsI 633 GAAGAC GAAGAC(2/6)
1062 GAAGAC
BcgI 804 CGAGAACGATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
990 CGATGTGTATGC
BpuAI 633 GAAGAC GAAGAC(2/6)
1062 GAAGAC
BsmAI 54 GTCTC GTCTC(1/5)
504 GTCTC
BsrBI 74 GAGCGG CCGCTC(-3/-3)
1255 GAGCGG
BsrDI 567 CATTGC GCAATG(2/0)
571 GCAATG
BsrI 1242 ACTGG ACTGG(1/-1)
1293 ACTGG
DpnII 564 GATC ^GATC
984 GATC
EarI 678 GAAGAG CTCTTC(1/4)
1149 GAAGAG
EcoT14I 434 CCAAGG C^C[AT][AT]GG
540 CCAAGG
FauI 868 CCCGC CCCGC(4/6)
1303 GCGGG
Ksp632I 678 GAAGAG CTCTTC(1/4)
1149 GAAGAG
MboI 564 GATC ^GATC
984 GATC
MslI 567 CATTGCAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
612 CATCATCATG
NdeII 564 GATC ^GATC
984 GATC
PleI 1013 GAGTC GAGTC(4/5)
1273 GAGTC
RsaI 105 GTAC GT^AC
1089 GTAC
Sau3AI 564 GATC ^GATC
984 GATC
StyI 434 CCAAGG C^C[AT][AT]GG
540 CCAAGG
TspRI 555 CACTG CA[GC]TG[ACGT][ACGT]^
855 CAGTG
XcmI 434 CCAAGGAATGTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
581 CCATCTTCATCATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 59 enzymes) #
#########################################
AciI AcyI AluI AvaI BalI
BanI BanII BbvI BcnI BlpI
BmyI BpmI Bpu1102I BsaHI BsaJI
BseRI BsgI BsiYI BslI BsmFI
BsoBI Bsp1286I BssKI BstF5I BstNI
Cac8I CelII Cfr13I DdeI EaeI
EcoRII Fnu4HI FokI HaeIII HapII
HgaI Hin1I HinfI HpaII HphI
ItaI MaeII MboII MluNI MnlI
MscI MspI MvaI MwoI NciI
NlaIII NlaIV NspI Sau96I ScrFI
SfaNI TaqI TseI TthHB8I