Restriction map for
pF1KB6459
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# NO CUT ENZYMES ( 165 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AflIII AgeI
Alw44I AocI Aor51HI ApaI ApaLI
AscI Asp718I AspI AvaI AviII
AvrII BalI BamHI BanI BbeI
BbrPI BbsI BbvCI BciVI BclI
BcnI BfrI BglI BglII BlnI
BlpI BpmI Bpu1102I BpuAI BsaAI
BsaHI BsaI BsaWI BseAI BsiEI
BsiWI BsmBI BsmFI BsmI BsoBI
Bsp1407I BspDI BspEI BspLU11I BsrBI
BsrDI BsrFI BsrGI BssHII Bst1107I
BstAPI BstBI BstEII BstF5I BstPI
BstUI BstXI BstZ17I Bsu36I Cac8I
CelII Cfr10I ClaI CpoI DraII
DraIII DrdI DsaI EagI EarI
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO109I EcoO65I EcoRI EcoT22I FauI
FbaI FokI FseI FspI HaeII
HapII HgaI Hin1I HincII HindII
HpaI HpaII HphI KasI KpnI
Ksp632I KspI MfeI MluI MluNI
MroI MscI MspA1I MspI MunI
MvnI NaeI NarI NciI NcoI
NdeI NgoMIV NheI NlaIV NotI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PpuMI PshAI Psp1406I PvuI
PvuII RsrII SacII SalI SapI
SbfI ScaI SexAI SfaNI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspBI SwaI
Tth111I XbaI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 57 enzymes) #
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AciI 486 GCGG CCGC(-3/-1)
AhdI 448 GACCACAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwI 903 GGATC GGATC(4/5)
AlwNI 464 CAGAGACTG CAG[ACGT][ACGT][ACGT]^CTG
Asp700I 710 GAAAGTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 448 GACCACAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaII 259 GGTCC G^G[AT]CC
BanII 185 GAGCTC G[GA]GC[CT]^C
BbvI 640 GCTGC GCAGC(8/12)
BcgI 822 GCACCTAAATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI 424 CTAG C^TAG
BsaBI 904 GATCTAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseRI 235 GAGGAG GAGGAG(10/8)
BsgI 461 GTGCAG GTGCAG(16/14)
BsmAI 466 GAGAC GTCTC(1/5)
BspMI 159 ACCTGC ACCTGC(4/8)
BsrI 469 ACTGG ACTGG(1/-1)
BssKI 398 CCAGG ^CC[ACGT]GG
BssSI 230 CTCGTG CACGAG(-5/-1)
BstNI 398 CCAGG CC^[AT]GG
BstYI 903 GGATCT [GA]^GATC[CT]
CfoI 820 GCGC GCG^C
Cfr13I 259 GGTCC G^G[ACGT]CC
DraI 852 TTTAAA TTT^AAA
EaeI 487 CGGCCA [CT]^GGCC[GA]
Eam1105I 448 GACCACAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRII 398 CCAGG ^CC[AT]GG
EcoRV 929 GATATC GAT^ATC
EcoT14I 664 CCAAGG C^C[AT][AT]GG
HhaI 820 GCGC GCG^C
HinP1I 820 GCGC G^CGC
MaeI 424 CTAG C^TAG
MaeII 683 ACGT A^CGT
MamI 904 GATCTAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 903 GGATCT [GA]^GATC[CT]
MvaI 398 CCAGG CC^[AT]GG
MwoI 414 GCTTTTGGTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
PflMI 784 CCACAGAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 140 GACTC GAGTC(4/5)
PstI 161 CTGCAG CTGCA^G
SacI 185 GAGCTC GAGCT^C
Sau96I 259 GGTCC G^G[ACGT]CC
ScrFI 398 CCAGG CC^[ACGT]GG
SfcI 161 CTGCAG C^T[GA][CT]AG
SspI 917 AATATT AAT^ATT
StuI 805 AGGCCT AGG^CCT
StyI 664 CCAAGG C^C[AT][AT]GG
TaqI 77 TCGA T^CGA
TfiI 390 GAATC G^A[AT]TC
TseI 640 GCTGC G^C[AT]GC
Tsp45I 46 GTGAC ^GT[GC]AC
TspRI 44 CAGTG CA[GC]TG[ACGT][ACGT]^
TthHB8I 77 TCGA T^CGA
Van91I 784 CCACAGAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 126 CCAAAAGTTACTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 903 GGATCT [GA]^GATC[CT]
XmnI 710 GAAAGTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 18 enzymes) #
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AseI 480 ATTAAT AT^TAAT
675 ATTAAT
AsnI 480 ATTAAT AT^TAAT
675 ATTAAT
BspHI 215 TCATGA T^CATGA
498 TCATGA
DdeI 255 CTTAG C^T[ACGT]AG
863 CTCAG
DpnII 79 GATC ^GATC
904 GATC
Fnu4HI 486 GCGGC GC^[ACGT]GC
640 GCTGC
HaeIII 488 GGCC GG^CC
806 GGCC
HindIII 178 AAGCTT A^AGCTT
412 AAGCTT
HinfI 140 GACTC G^A[ACGT]TC
390 GAATC
ItaI 486 GCGGC GC^[ACGT]GC
640 GCTGC
MaeIII 46 GTGAC ^GT[ACGT]AC
132 GTTAC
MboI 79 GATC ^GATC
904 GATC
MslI 216 CATGATGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
741 CACCATTATG
NdeII 79 GATC ^GATC
904 GATC
PshBI 480 ATTAAT AT^TAAT
675 ATTAAT
RcaI 215 TCATGA T^CATGA
498 TCATGA
Sau3AI 79 GATC ^GATC
904 GATC
SmlI 94 CTCAAG C^T[CT][GA]AG
871 CTCAAG
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# 3 OR MORE CUT ENZYMES ( 18 enzymes) #
#########################################
AcsI AfaI AluI ApoI AspHI
BmyI BsaJI BsiHKAI BsiYI BslI
Bsp1286I MboII MnlI MseI NlaIII
RsaI Tru9I Tsp509I