Restriction map for
pF1KB0442
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# NO CUT ENZYMES ( 88 enzymes) #
##################################
AatII AclI AcyI AflII AgeI
AhdI AocI AscI AseI AsnI
Asp700I AspEI AviII BamHI BbeI
BcgI BclI BfrI BglII BsaHI
BsiWI Bsp1407I BspDI BspHI BspLU11I
BsrBI BsrFI BsrGI BssHII Bst1107I
BstAPI BstBI BstZ17I Bsu36I Cfr10I
ClaI CpoI DraI DraIII DrdI
Eam1105I Eco81I EcoRV FbaI FseI
FspI Hin1I HincII HindII HpaI
KasI MfeI MluI MunI NaeI
NarI NdeI NgoMIV NheI NotI
NruI NspV PacI PinAI PmeI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SacI SalI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SnaBI SpeI Sse8387I SspBI SspI
SwaI XbaI XmnI
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# ONE CUT ENZYMES ( 54 enzymes) #
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Acc65I 2308 GGTACC G^GTACC
AccI 1990 GTCTAC GT^[AC][GT]AC
AccIII 2559 TCCGGA T^CCGGA
Alw44I 1450 GTGCAC G^TGCAC
ApaLI 1450 GTGCAC G^TGCAC
Asp718I 2308 GGTACC G^GTACC
AspI 2605 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 1362 CCTAGG C^CTAGG
BalI 1475 TGGCCA TGG^CCA
BciVI 2515 GGATAC GTATCC(6/5)
BglI 710 GCCCCGGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 1362 CCTAGG C^CTAGG
BlpI 1739 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 1739 GCTCAGC GC^T[ACGT]AGC
BsaI 2660 GAGACC GGTCTC(1/5)
BseAI 2559 TCCGGA T^CCGGA
BsiEI 2434 CGGCCG CG[GA][CT]^CG
BsmI 213 GCATTC GAATGC(1/-1)
BspEI 2559 TCCGGA T^CCGGA
BssSI 531 CTCGTG CACGAG(-5/-1)
BstEII 1342 GGTCACC G^GT[ACGT]ACC
BstPI 1342 GGTCACC G^GT[ACGT]ACC
BstYI 684 AGATCC [GA]^GATC[CT]
CelII 1739 GCTCAGC GC^T[ACGT]AGC
EagI 2434 CGGCCG C^GGCCG
EclXI 2434 CGGCCG C^GGCCG
Eco52I 2434 CGGCCG C^GGCCG
EcoNI 1090 CCTCCAGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 1342 GGTCACC G^GT[ACGT]ACC
EcoRI 1590 GAATTC G^AATTC
EcoT22I 211 ATGCAT ATGCA^T
HgaI 1932 GACGC GACGC(5/10)
HindIII 1582 AAGCTT A^AGCTT
KpnI 2308 GGTACC GGTAC^C
KspI 778 CCGCGG CCGC^GG
MflI 684 AGATCC [GA]^GATC[CT]
MluNI 1475 TGGCCA TGG^CCA
MroI 2559 TCCGGA T^CCGGA
MscI 1475 TGGCCA TGG^CCA
MseI 38 TTAA T^TAA
NcoI 670 CCATGG C^CATGG
NsiI 211 ATGCAT ATGCA^T
PaeR7I 331 CTCGAG C^TCGAG
PflFI 2605 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
SacII 778 CCGCGG CCGC^GG
SapI 2692 GAAGAGC GCTCTTC(1/4)
SmlI 331 CTCGAG C^T[CT][GA]AG
StuI 1766 AGGCCT AGG^CCT
TfiI 1574 GAATC G^A[AT]TC
Tru9I 38 TTAA T^TAA
Tth111I 2605 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 727 CCAGAGGATAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 331 CTCGAG C^TCGAG
XhoII 684 AGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AcsI 34 GAATTT [GA]^AATT[CT]
1590 GAATTC
AlwI 685 GATCC GGATC(4/5)
1259 GGATC
AlwNI 1747 CAGGAGCTG CAG[ACGT][ACGT][ACGT]^CTG
1779 CAGTGCCTG
Aor51HI 387 AGCGCT AGC^GCT
1736 AGCGCT
ApoI 34 GAATTT [GA]^AATT[CT]
1590 GAATTC
BbrPI 1643 CACGTG CAC^GTG
1669 CACGTG
BbsI 984 GAAGAC GAAGAC(2/6)
2182 GAAGAC
BbvCI 1254 GCTGAGG CCTCAGC(-5/-2)
2509 GCTGAGG
BpuAI 984 GAAGAC GAAGAC(2/6)
2182 GAAGAC
BsaAI 1643 CACGTG [CT]AC^GT[GA]
1669 CACGTG
BsaBI 1395 GATGTTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2491 GATGTGCATC
BsaWI 2079 ACCGGA [AT]^CCGG[AT]
2559 TCCGGA
BsgI 167 CTGCAC GTGCAG(16/14)
2678 CTGCAC
BsmBI 1695 CGTCTC CGTCTC(1/5)
1618 GAGACG
Eco47III 387 AGCGCT AGC^GCT
1736 AGCGCT
MaeII 1644 ACGT A^CGT
1670 ACGT
MamI 1395 GATGTTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2491 GATGTGCATC
MslI 1509 CACCAAGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
2130 CACTGACATG
NspI 209 GCATGC [GA]CATG^[CT]
2231 GCATGC
PflMI 505 CCAAGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2533 CCAGGCTATGG
PmaCI 1643 CACGTG CAC^GTG
1669 CACGTG
PmlI 1643 CACGTG CAC^GTG
1669 CACGTG
SphI 209 GCATGC GCATG^C
2231 GCATGC
Tsp509I 35 AATT ^AATT
1591 AATT
Van91I 505 CCAAGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2533 CCAGGCTATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 91 enzymes) #
#########################################
AccII AciI AfaI AflIII AluI
ApaI AspHI AvaI AvaII BanI
BanII BbvI BcnI BfaI BmyI
BpmI BsaJI BseRI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BspMI BsrDI BsrI BssKI BstF5I
BstNI BstUI BstXI Cac8I CfoI
Cfr13I DdeI DpnII DraII DsaI
EaeI EarI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HhaI HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeI MaeIII
MboI MboII MnlI MspA1I MspI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV PleI PpuMI PstI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmaI StyI TaqI
TseI Tsp45I TspRI TthHB8I XmaCI
XmaI