Restriction map for
pF1KB0449
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# NO CUT ENZYMES ( 120 enzymes) #
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Acc65I AccI AccIII AclI AflII
AflIII AgeI AhdI AocI Aor51HI
ApaI AscI AseI AsnI Asp700I
Asp718I AspEI AspI AviII AvrII
BbeI BbrPI BbvCI BcgI BfaI
BfrI BglI BlnI BsaBI BseAI
BsiEI BsiWI BsmI Bsp1407I BspDI
BspEI BspLU11I BsrDI BsrGI BssHII
Bst1107I BstBI BstZ17I Bsu36I ClaI
CpoI DraI DraIII DrdI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoT22I FseI FspI HgaI HincII
HindII HindIII HpaI KasI KpnI
KspI MaeI MamI MfeI MluI
MroI MunI NaeI NarI NdeI
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PflMI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PstI PvuI RsrII
SacI SacII SalI SapI SbfI
SexAI SfiI SfoI SfuI SgrAI
SmlI SnaBI SpeI Sse8387I SspBI
StuI SwaI TaqI Tth111I TthHB8I
Van91I XbaI XcmI XhoI XmnI
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# ONE CUT ENZYMES ( 41 enzymes) #
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AatII 274 GACGTC GACGT^C
AccII 1170 CGCG CG^CG
AcyI 274 GACGTC G[GA]^CG[CT]C
Alw44I 1294 GTGCAC G^TGCAC
ApaLI 1294 GTGCAC G^TGCAC
BamHI 940 GGATCC G^GATCC
BanI 457 GGCACC G^G[CT][GA]CC
BciVI 1182 GGATAC GTATCC(6/5)
BclI 2 TGATCA T^GATCA
BglII 354 AGATCT A^GATCT
BlpI 369 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 369 GCTCAGC GC^T[ACGT]AGC
BsaAI 430 TACGTG [CT]AC^GT[GA]
BsaHI 274 GACGTC G[GA]^CG[CT]C
BsaWI 1274 TCCGGT [AT]^CCGG[AT]
BsmBI 1087 GAGACG CGTCTC(1/5)
BspMI 809 ACCTGC ACCTGC(4/8)
BsrFI 116 ACCGGC [GA]^CCGG[CT]
BssSI 535 CACGAG CACGAG(-5/-1)
BstAPI 1246 GCAGAGGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 903 GGTGACC G^GT[ACGT]ACC
BstPI 903 GGTGACC G^GT[ACGT]ACC
BstUI 1170 CGCG CG^CG
BstXI 893 CCAATGAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 369 GCTCAGC GC^T[ACGT]AGC
Cfr10I 116 ACCGGC [GA]^CCGG[CT]
EcoNI 312 CCTGCCCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 903 GGTGACC G^GT[ACGT]ACC
EcoRV 304 GATATC GAT^ATC
FbaI 2 TGATCA T^GATCA
Hin1I 274 GACGTC G[GA]^CG[CT]C
MseI 26 TTAA T^TAA
MvnI 1170 CGCG CG^CG
PvuII 742 CAGCTG CAG^CTG
ScaI 950 AGTACT AGT^ACT
SmaI 1023 CCCGGG CCC^GGG
SphI 713 GCATGC GCATG^C
SspI 601 AATATT AAT^ATT
Tru9I 26 TTAA T^TAA
XmaCI 1023 CCCGGG C^CCGGG
XmaI 1023 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 23 enzymes) #
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AcsI 85 GAATTC [GA]^AATT[CT]
322 GAATTC
ApoI 85 GAATTC [GA]^AATT[CT]
322 GAATTC
AspHI 532 GAGCAC G[AT]GC[AT]^C
1294 GTGCAC
AvaI 1023 CCCGGG C^[CT]CG[GA]G
1069 CTCGGG
BalI 857 TGGCCA TGG^CCA
1001 TGGCCA
BanII 348 GAGCCC G[GA]GC[CT]^C
1026 GGGCTC
BbsI 1306 GAAGAC GAAGAC(2/6)
472 GTCTTC
BpmI 1345 CTCCAG CTGGAG(16/14)
529 CTGGAG
BpuAI 1306 GAAGAC GAAGAC(2/6)
472 GTCTTC
BsiHKAI 532 GAGCAC G[AT]GC[AT]^C
1294 GTGCAC
BsoBI 1023 CCCGGG C^[CT]CG[GA]G
1069 CTCGGG
BspHI 5 TCATGA T^CATGA
707 TCATGA
BsrBI 367 CCGCTC CCGCTC(-3/-3)
1156 CCGCTC
DsaI 266 CCATGG C^C[GA][CT]GG
299 CCATGG
EarI 1018 CTCTTC CTCTTC(1/4)
1084 GAAGAG
EcoRI 85 GAATTC G^AATTC
322 GAATTC
Ksp632I 1018 CTCTTC CTCTTC(1/4)
1084 GAAGAG
MluNI 857 TGGCCA TGG^CCA
1001 TGGCCA
MscI 857 TGGCCA TGG^CCA
1001 TGGCCA
NcoI 266 CCATGG C^CATGG
299 CCATGG
PleI 1233 GAGTC GAGTC(4/5)
1260 GAGTC
RcaI 5 TCATGA T^CATGA
707 TCATGA
TfiI 73 GAATC G^A[AT]TC
1225 GAATC
#########################################
# 3 OR MORE CUT ENZYMES ( 74 enzymes) #
#########################################
AciI AfaI AluI AlwI AlwNI
AvaII BbvI BcnI BmyI BsaI
BsaJI BseRI BsgI BsiYI BslI
BsmAI BsmFI Bsp1286I BsrI BssKI
BstF5I BstNI BstYI Cac8I CfoI
Cfr13I DdeI DpnII DraII EaeI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HhaI
HinP1I HinfI HpaII HphI ItaI
MaeII MaeIII MboI MboII MflI
MnlI MslI MspA1I MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
NspI PpuMI RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI StyI TseI
Tsp45I Tsp509I TspRI XhoII