Restriction map for
pF1KB0453
##################################
# NO CUT ENZYMES ( 134 enzymes) #
##################################
AatII AccII AccIII AclI AflII
AgeI AhdI Alw44I AocI Aor51HI
ApaI ApaLI AscI AseI AsnI
AspEI AspI AviII AvrII BalI
BamHI BanII BbeI BbrPI BbsI
BciVI BclI BfaI BfrI BglI
BglII BlnI BlpI Bpu1102I BpuAI
BsaBI BsaI BsaWI BseAI BsiEI
BsiWI BsmAI BsmBI Bsp1407I BspEI
BspLU11I BspMI BsrBI BsrGI BssHII
BssSI Bst1107I BstAPI BstEII BstPI
BstUI BstZ17I Bsu36I CelII CpoI
DraI DraIII DrdI DsaI EaeI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoNI EcoO65I EcoT14I EcoT22I
FbaI FseI FspI HindIII HpaI
KasI KspI MaeI MamI MfeI
MluI MluNI MroI MscI MunI
MvnI NarI NcoI NdeI NheI
NotI NruI NsiI PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PstI PvuI
PvuII RsrII SacI SacII SalI
SapI SbfI ScaI SfcI SfiI
SfoI SgrAI SnaBI SpeI SphI
Sse8387I SspBI StuI StyI SwaI
Tth111I XbaI XcmI XhoI
###################################
# ONE CUT ENZYMES ( 40 enzymes) #
###################################
Acc65I 1028 GGTACC G^GTACC
AccI 715 GTCTAC GT^[AC][GT]AC
AcyI 1430 GGCGTC G[GA]^CG[CT]C
AfaI 1029 GTAC GT^AC
AflIII 158 ACGTGT A^C[GA][CT]GT
Asp700I 438 GAATTCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 1028 GGTACC G^GTACC
AspHI 314 GTGCTC G[AT]GC[AT]^C
BbvCI 1473 CCTCAGC CCTCAGC(-5/-2)
BcgI 1151 GCAATGTCCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BsaAI 157 TACGTG [CT]AC^GT[GA]
BsaHI 1430 GGCGTC G[GA]^CG[CT]C
BsgI 1108 GTGCAG GTGCAG(16/14)
BsiHKAI 314 GTGCTC G[AT]GC[AT]^C
BsmI 583 GAATGC GAATGC(1/-1)
BspDI 1073 ATCGAT AT^CGAT
BspHI 725 TCATGA T^CATGA
BstXI 497 CCAATATTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
ClaI 1073 ATCGAT AT^CGAT
DraII 1100 AGGACCT [GA]G^G[ACGT]CC[CT]
EcoO109I 1100 AGGACCT [GA]G^G[ACGT]CC[CT]
EcoRI 438 GAATTC G^AATTC
EcoRV 1076 GATATC GAT^ATC
FauI 828 GCGGG CCCGC(4/6)
HaeII 324 GGCGCT [GA]GCGC^[CT]
HgaI 1431 GCGTC GACGC(5/10)
Hin1I 1430 GGCGTC G[GA]^CG[CT]C
HincII 679 GTCAAC GT[CT]^[GA]AC
HindII 679 GTCAAC GT[CT]^[GA]AC
KpnI 1028 GGTACC GGTAC^C
MspA1I 1111 CAGCGG C[AC]G^C[GT]G
NspI 1496 ACATGC [GA]CATG^[CT]
PflMI 1098 CCAGGACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 1100 AGGACCT [GA]G^G[AT]CC[CT]
RcaI 725 TCATGA T^CATGA
RsaI 1029 GTAC GT^AC
SexAI 1103 ACCTGGT A^CC[AT]GGT
SspI 499 AATATT AAT^ATT
Van91I 1098 CCAGGACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmnI 438 GAATTCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AcsI 438 GAATTC [GA]^AATT[CT]
859 AAATTT
AlwNI 1099 CAGGACCTG CAG[ACGT][ACGT][ACGT]^CTG
1111 CAGCGGCTG
ApoI 438 GAATTC [GA]^AATT[CT]
859 AAATTT
AvaII 917 GGACC G^G[AT]CC
1101 GGACC
BanI 945 GGTGCC G^G[CT][GA]CC
1028 GGTACC
BsmFI 410 GGGAC GGGAC(10/14)
199 GTCCC
BsrDI 489 CATTGC GCAATG(2/0)
1151 GCAATG
BsrFI 764 GCCGGC [GA]^CCGG[CT]
1457 GCCGGC
BstBI 62 TTCGAA TT^CGAA
1416 TTCGAA
BstF5I 699 CATCC GGATG(2/0)
1382 GGATG
BstYI 299 AGATCC [GA]^GATC[CT]
633 GGATCT
Cfr10I 764 GCCGGC [GA]^CCGG[CT]
1457 GCCGGC
FokI 699 CATCC GGATG(9/13)
1382 GGATG
HphI 651 GGTGA GGTGA(8/7)
808 GGTGA
MflI 299 AGATCC [GA]^GATC[CT]
633 GGATCT
NaeI 764 GCCGGC GCC^GGC
1457 GCCGGC
NgoMIV 764 GCCGGC G^CCGGC
1457 GCCGGC
NspV 62 TTCGAA TT^CGAA
1416 TTCGAA
SfuI 62 TTCGAA TT^CGAA
1416 TTCGAA
SmaI 143 CCCGGG CCC^GGG
398 CCCGGG
SmlI 1236 CTTGAG C^T[CT][GA]AG
1465 CTCAAG
Tsp45I 809 GTGAC ^GT[GC]AC
1337 GTGAC
XhoII 299 AGATCC [GA]^GATC[CT]
633 GGATCT
XmaCI 143 CCCGGG C^CCGGG
398 CCCGGG
XmaI 143 CCCGGG C^CCGGG
398 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 59 enzymes) #
#########################################
AciI AluI AlwI AvaI BbvI
BcnI BmyI BpmI BsaJI BseRI
BsiYI BslI BsoBI Bsp1286I BsrI
BssKI BstNI Cac8I CfoI Cfr13I
DdeI DpnII EarI EcoRII Fnu4HI
HaeIII HapII HhaI HinP1I HinfI
HpaII ItaI Ksp632I MaeII MaeIII
MboI MboII MnlI MseI MslI
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV PleI Sau3AI Sau96I
ScrFI SfaNI TaqI TfiI Tru9I
TseI Tsp509I TspRI TthHB8I