Restriction map for
pF1KB0458
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# NO CUT ENZYMES ( 94 enzymes) #
##################################
AatII Acc65I AccI AclI AflII
AgeI AhdI Aor51HI ApaI AscI
AseI AsnI Asp718I AspEI BbeI
BbrPI BbvCI BcgI BciVI BclI
BfrI BglI BlpI Bpu1102I BsaBI
BsiEI BsiWI BsmBI Bsp1407I BspHI
BsrBI BsrFI BsrGI BssHII Bst1107I
BstAPI BstEII BstPI BstZ17I CelII
Cfr10I CpoI DraI EagI Eam1105I
EclXI Eco47III Eco52I EcoNI EcoO65I
EcoRV FbaI FseI HaeII HpaI
KasI KpnI KspI MamI MluI
NaeI NarI NgoMIV NheI NotI
NruI PacI PaeR7I PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI RcaI RsrII SacII SalI
SbfI ScaI SexAI SfiI SfoI
SgrAI SnaBI SpeI Sse8387I SspBI
StuI SwaI XbaI XhoI
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# ONE CUT ENZYMES ( 43 enzymes) #
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AcyI 1388 GACGCC G[GA]^CG[CT]C
Alw44I 2581 GTGCAC G^TGCAC
AlwNI 686 CAGAAGCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 365 CCTTAGG CC^T[ACGT]AGG
ApaLI 2581 GTGCAC G^TGCAC
AspI 2002 GACCACGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 2713 TGCGCA TGC^GCA
AvrII 549 CCTAGG C^CTAGG
BamHI 2246 GGATCC G^GATCC
BbsI 2634 GAAGAC GAAGAC(2/6)
BlnI 549 CCTAGG C^CTAGG
BpmI 2593 CTCCAG CTGGAG(16/14)
BpuAI 2634 GAAGAC GAAGAC(2/6)
BsaAI 1610 TACGTG [CT]AC^GT[GA]
BsaHI 1388 GACGCC G[GA]^CG[CT]C
BsmI 1439 GAATGC GAATGC(1/-1)
BspDI 1651 ATCGAT AT^CGAT
BspLU11I 1336 ACATGT A^CATGT
BstBI 2170 TTCGAA TT^CGAA
Bsu36I 365 CCTTAGG CC^T[ACGT]AGG
ClaI 1651 ATCGAT AT^CGAT
Eco81I 365 CCTTAGG CC^T[ACGT]AGG
EcoT22I 2281 ATGCAT ATGCA^T
FauI 2147 GCGGG CCCGC(4/6)
FspI 2713 TGCGCA TGC^GCA
HgaI 1388 GACGC GACGC(5/10)
Hin1I 1388 GACGCC G[GA]^CG[CT]C
HincII 2269 GTTGAC GT[CT]^[GA]AC
HindII 2269 GTTGAC GT[CT]^[GA]AC
HindIII 1453 AAGCTT A^AGCTT
MfeI 1040 CAATTG C^AATTG
MunI 1040 CAATTG C^AATTG
NcoI 656 CCATGG C^CATGG
NdeI 1344 CATATG CA^TATG
NsiI 2281 ATGCAT ATGCA^T
NspV 2170 TTCGAA TT^CGAA
PflFI 2002 GACCACGTC GAC[ACGT]^[ACGT][ACGT]GTC
PstI 902 CTGCAG CTGCA^G
SapI 1800 GAAGAGC GCTCTTC(1/4)
SfuI 2170 TTCGAA TT^CGAA
SphI 1115 GCATGC GCATG^C
SspI 1327 AATATT AAT^ATT
Tth111I 2002 GACCACGTC GAC[ACGT]^[ACGT][ACGT]GTC
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AccIII 444 TCCGGA T^CCGGA
1968 TCCGGA
AflIII 158 ACGTGT A^C[GA][CT]GT
1336 ACATGT
Asp700I 1732 GAAGAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1809 GAAAAAGTTC
BalI 110 TGGCCA TGG^CCA
1688 TGGCCA
BglII 918 AGATCT A^GATCT
1781 AGATCT
BsaWI 444 TCCGGA [AT]^CCGG[AT]
1968 TCCGGA
BseAI 444 TCCGGA T^CCGGA
1968 TCCGGA
BsgI 433 GTGCAG GTGCAG(16/14)
1567 GTGCAG
BspEI 444 TCCGGA T^CCGGA
1968 TCCGGA
CfoI 188 GCGC GCG^C
2714 GCGC
DrdI 157 GACGTGTTTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
554 GACCAGAGAGTC
DsaI 656 CCATGG C^C[GA][CT]GG
1701 CCGTGG
EaeI 110 TGGCCA [CT]^GGCC[GA]
1688 TGGCCA
EarI 37 GAAGAG CTCTTC(1/4)
1800 GAAGAG
EcoRI 2494 GAATTC G^AATTC
2665 GAATTC
HhaI 188 GCGC GCG^C
2714 GCGC
HinP1I 188 GCGC G^CGC
2714 GCGC
Ksp632I 37 GAAGAG CTCTTC(1/4)
1800 GAAGAG
MluNI 110 TGGCCA TGG^CCA
1688 TGGCCA
MroI 444 TCCGGA T^CCGGA
1968 TCCGGA
MscI 110 TGGCCA TGG^CCA
1688 TGGCCA
PpuMI 842 AGGACCC [GA]G^G[AT]CC[CT]
1580 AGGACCT
SmaI 1122 CCCGGG CCC^GGG
2048 CCCGGG
SmlI 1231 CTTGAG C^T[CT][GA]AG
2485 CTCAAG
XcmI 119 CCAAGGCAGAAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2420 CCACCCTGAAATTGG
XmaCI 1122 CCCGGG C^CCGGG
2048 CCCGGG
XmaI 1122 CCCGGG C^CCGGG
2048 CCCGGG
XmnI 1732 GAAGAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1809 GAAAAAGTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 93 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
AlwI ApoI AspHI AvaI AvaII
BanI BanII BbvI BcnI BfaI
BmyI BsaI BsaJI BseRI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BspMI BsrDI BsrI BssKI
BssSI BstF5I BstNI BstUI BstXI
BstYI Cac8I Cfr13I DdeI DpnII
DraII DraIII EcoO109I EcoRII EcoT14I
Fnu4HI FokI HaeIII HapII HinfI
HpaII HphI ItaI MaeI MaeII
MaeIII MboI MboII MflI MnlI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV NspI PflMI PleI PvuII
RsaI SacI Sau3AI Sau96I ScrFI
SfaNI SfcI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I XhoII