Restriction map for
pF1KB0462
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# NO CUT ENZYMES ( 84 enzymes) #
##################################
Acc65I AccI AclI AflII AhdI
AscI AseI AsnI Asp700I Asp718I
AspEI AspI AviII AvrII BbeI
BbrPI BcgI BfrI BlnI BspDI
BspHI BspLU11I BsrBI BssHII Bst1107I
BstBI BstZ17I ClaI CpoI DraI
DraIII Eam1105I EcoRI EcoRV EcoT22I
FseI FspI HincII HindII HindIII
HpaI KasI KpnI KspI MfeI
MluI MslI MunI NarI NdeI
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PmaCI PmeI
PmlI PshBI Psp1406I PvuI PvuII
RcaI RsrII SacII SalI SbfI
ScaI SexAI SfoI SfuI SnaBI
SpeI SphI Sse8387I SspI SwaI
Tth111I XbaI XhoI XmnI
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# ONE CUT ENZYMES ( 41 enzymes) #
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AatII 25 GACGTC GACGT^C
AflIII 2609 ACACGT A^C[GA][CT]GT
AgeI 982 ACCGGT A^CCGGT
Alw44I 169 GTGCAC G^TGCAC
AocI 252 CCTCAGG CC^T[ACGT]AGG
ApaI 364 GGGCCC GGGCC^C
ApaLI 169 GTGCAC G^TGCAC
BbvCI 1566 GCTGAGG CCTCAGC(-5/-2)
BciVI 1349 GTATCC GTATCC(6/5)
BclI 2273 TGATCA T^GATCA
BfaI 1469 CTAG C^TAG
BglII 2849 AGATCT A^GATCT
BsaAI 2610 CACGTA [CT]AC^GT[GA]
BsaBI 2145 GATCGACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiWI 2612 CGTACG C^GTACG
BstAPI 594 GCAGGGGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 1611 GGTCACC G^GT[ACGT]ACC
BstPI 1611 GGTCACC G^GT[ACGT]ACC
Bsu36I 252 CCTCAGG CC^T[ACGT]AGG
DrdI 1204 GACCACATGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 2515 CGGCCG C^GGCCG
EclXI 2515 CGGCCG C^GGCCG
Eco52I 2515 CGGCCG C^GGCCG
Eco81I 252 CCTCAGG CC^T[ACGT]AGG
EcoO65I 1611 GGTCACC G^GT[ACGT]ACC
FbaI 2273 TGATCA T^GATCA
MaeI 1469 CTAG C^TAG
MamI 2145 GATCGACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NaeI 2890 GCCGGC GCC^GGC
NcoI 851 CCATGG C^CATGG
NgoMIV 2890 GCCGGC G^CCGGC
PinAI 982 ACCGGT A^CCGGT
PshAI 1714 GACAAAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 1457 CTGCAG CTGCA^G
SacI 901 GAGCTC GAGCT^C
SapI 1809 GCTCTTC GCTCTTC(1/4)
SgrAI 981 CACCGGTG C[GA]^CCGG[CT]G
SmaI 2910 CCCGGG CCC^GGG
XcmI 644 CCAAGGCCCTCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 2910 CCCGGG C^CCGGG
XmaI 2910 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AcsI 1385 GAATTT [GA]^AATT[CT]
2775 AAATTC
Aor51HI 17 AGCGCT AGC^GCT
129 AGCGCT
ApoI 1385 GAATTT [GA]^AATT[CT]
2775 AAATTC
BamHI 378 GGATCC G^GATCC
1328 GGATCC
BbsI 1528 GAAGAC GAAGAC(2/6)
2799 GTCTTC
BlpI 125 GCTCAGC GC^T[ACGT]AGC
274 GCTCAGC
Bpu1102I 125 GCTCAGC GC^T[ACGT]AGC
274 GCTCAGC
BpuAI 1528 GAAGAC GAAGAC(2/6)
2799 GTCTTC
BsaI 1464 GGTCTC GGTCTC(1/5)
2763 GGTCTC
BsiEI 51 CGACCG CG[GA][CT]^CG
2515 CGGCCG
BsmBI 2781 CGTCTC CGTCTC(1/5)
318 GAGACG
Bsp1407I 866 TGTACA T^GTACA
2873 TGTACA
BsrDI 2705 GCAATG GCAATG(2/0)
2862 GCAATG
BsrGI 866 TGTACA T^GTACA
2873 TGTACA
BssSI 100 CTCGTG CACGAG(-5/-1)
2857 CTCGTG
BstXI 938 CCAGCGACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1418 CCACGAAGCTGG
CelII 125 GCTCAGC GC^T[ACGT]AGC
274 GCTCAGC
Eco47III 17 AGCGCT AGC^GCT
129 AGCGCT
MspA1I 54 CCGCTG C[AC]G^C[GT]G
409 CAGCGG
NspI 1520 GCATGT [GA]CATG^[CT]
2655 GCATGT
PflMI 2331 CCAGAACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2622 CCACCGGCTGG
SfcI 238 CTACAG C^T[GA][CT]AG
1457 CTGCAG
SspBI 866 TGTACA T^GTACA
2873 TGTACA
StuI 609 AGGCCT AGG^CCT
2386 AGGCCT
Van91I 2331 CCAGAACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2622 CCACCGGCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 108 enzymes) #
#########################################
AccII AccIII AciI AcyI AfaI
AluI AlwI AlwNI AspHI AvaI
AvaII BalI BanI BanII BbvI
BcnI BglI BmyI BpmI BsaHI
BsaJI BsaWI BseAI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsmI BsoBI Bsp1286I BspEI BspMI
BsrFI BsrI BssKI BstF5I BstNI
BstUI BstYI Cac8I CfoI Cfr10I
Cfr13I DdeI DpnII DraII DsaI
EaeI EarI EcoNI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeII MaeIII MboI MboII
MflI MluNI MnlI MroI MscI
MseI MspI MvaI MvnI MwoI
NciI NdeII NlaIII NlaIV PleI
PpuMI RsaI Sau3AI Sau96I ScrFI
SfaNI SfiI SmlI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XhoII