Restriction map for
pF1KB0902
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# NO CUT ENZYMES ( 158 enzymes) #
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AatII Acc65I AccII AccIII AclI
AcsI AcyI AflII AgeI Alw44I
AlwI AlwNI AocI Aor51HI ApaI
ApaLI ApoI AscI AseI AsnI
Asp718I AspHI AspI AvaI AviII
BamHI BanI BanII BbeI BbrPI
BbsI BbvCI BcgI BcnI BfrI
BglI BglII BlpI BpmI Bpu1102I
BpuAI BsaAI BsaBI BsaHI BseAI
BsiEI BsiHKAI BsiWI BsmBI BsoBI
Bsp1407I BspDI BspEI BspHI BspLU11I
BsrBI BsrDI BsrFI BsrGI BssHII
BssSI Bst1107I BstBI BstEII BstPI
BstUI BstYI BstZ17I Bsu36I CelII
CfoI Cfr10I ClaI CpoI DrdI
EagI EclXI Eco47III Eco52I Eco81I
EcoO65I EcoRI EcoRV EcoT22I FseI
FspI HaeII HgaI HhaI Hin1I
HinP1I HincII HindII HindIII HpaI
KasI KpnI KspI MamI MfeI
MflI MluI MroI MunI MvnI
NaeI NarI NciI NdeI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PflMI PinAI
PleI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PstI PvuI RcaI
RsrII SacI SacII SalI SbfI
ScaI SexAI SfaNI SfcI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspBI SspI
SwaI Tth111I Van91I XbaI XhoI
XhoII XmaCI XmaI
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# ONE CUT ENZYMES ( 48 enzymes) #
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AfaI 807 GTAC GT^AC
AflIII 571 ACACGT A^C[GA][CT]GT
AhdI 85 GACCCCGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspEI 85 GACCCCGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvrII 405 CCTAGG C^CTAGG
BalI 602 TGGCCA TGG^CCA
BciVI 495 GTATCC GTATCC(6/5)
BclI 666 TGATCA T^GATCA
BfaI 406 CTAG C^TAG
BlnI 405 CCTAGG C^CTAGG
BsaI 417 GAGACC GGTCTC(1/5)
BsaWI 656 ACCGGA [AT]^CCGG[AT]
BsgI 799 GTGCAG GTGCAG(16/14)
BsmAI 417 GAGAC GTCTC(1/5)
BspMI 544 ACCTGC ACCTGC(4/8)
BstAPI 771 GCACAGCGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
DdeI 427 CTGAG C^T[ACGT]AG
DraI 682 TTTAAA TTT^AAA
DraII 83 AGGACCC [GA]G^G[ACGT]CC[CT]
DraIII 772 CACAGCGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 221 CCATGG C^C[GA][CT]GG
EaeI 602 TGGCCA [CT]^GGCC[GA]
Eam1105I 85 GACCCCGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 39 CCTTCAGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 83 AGGACCC [GA]G^G[ACGT]CC[CT]
FauI 510 CCCGC CCCGC(4/6)
FbaI 666 TGATCA T^GATCA
HapII 657 CCGG C^CGG
HinfI 751 GAATC G^A[ACGT]TC
HpaII 657 CCGG C^CGG
MaeI 406 CTAG C^TAG
MluNI 602 TGGCCA TGG^CCA
MscI 602 TGGCCA TGG^CCA
MseI 683 TTAA T^TAA
MslI 732 CATCTGTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI 657 CCGG C^CGG
NcoI 221 CCATGG C^CATGG
NspI 674 GCATGT [GA]CATG^[CT]
PpuMI 83 AGGACCC [GA]G^G[AT]CC[CT]
RsaI 807 GTAC GT^AC
SapI 487 GAAGAGC GCTCTTC(1/4)
SmlI 414 CTTGAG C^T[CT][GA]AG
StuI 878 AGGCCT AGG^CCT
TaqI 246 TCGA T^CGA
TfiI 751 GAATC G^A[AT]TC
Tru9I 683 TTAA T^TAA
TthHB8I 246 TCGA T^CGA
XcmI 590 CCAACTTGCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 20 enzymes) #
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AccI 349 GTAGAC GT^[AC][GT]AC
439 GTCTAC
Asp700I 702 GAAACTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
862 GAAAGCATTC
BmyI 106 GGGCAC G[AGT]GC[ACT]^C
633 GTGCCC
BsmI 267 GAATGC GAATGC(1/-1)
866 GCATTC
Bsp1286I 106 GGGCAC G[AGT]GC[ACT]^C
633 GTGCCC
BsrI 459 ACTGG ACTGG(1/-1)
552 CCAGT
BstXI 62 CCATCTGCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
731 CCATCTGTGTGG
Cac8I 600 GCTGGC GC[ACGT]^[ACGT]GC
776 GCGTGC
DpnII 391 GATC ^GATC
667 GATC
EcoT14I 221 CCATGG C^C[AT][AT]GG
405 CCTAGG
HphI 319 GGTGA GGTGA(8/7)
542 TCACC
MaeII 347 ACGT A^CGT
573 ACGT
MaeIII 130 GTGAC ^GT[ACGT]AC
320 GTGAC
MboI 391 GATC ^GATC
667 GATC
NdeII 391 GATC ^GATC
667 GATC
Sau3AI 391 GATC ^GATC
667 GATC
StyI 221 CCATGG C^C[AT][AT]GG
405 CCTAGG
Tsp45I 130 GTGAC ^GT[GC]AC
320 GTGAC
TspRI 324 CAGTG CA[GC]TG[ACGT][ACGT]^
553 CAGTG
XmnI 702 GAAACTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
862 GAAAGCATTC
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# 3 OR MORE CUT ENZYMES ( 32 enzymes) #
#########################################
AciI AluI AvaII BbvI BsaJI
BseRI BsiYI BslI BsmFI BssKI
BstF5I BstNI Cfr13I EarI EcoRII
Fnu4HI FokI HaeIII ItaI Ksp632I
MboII MnlI MspA1I MvaI MwoI
NlaIII NlaIV PvuII Sau96I ScrFI
TseI Tsp509I