Restriction map for
pF1KB0903
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# NO CUT ENZYMES ( 207 enzymes) #
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AatII Acc65I AccI AccII AccIII
AciI AclI AcyI AfaI AflII
AflIII AgeI AhdI AluI Alw44I
AlwI AlwNI AocI Aor51HI ApaI
ApaLI AscI AseI AsnI Asp700I
Asp718I AspEI AspHI AviII AvrII
BalI BamHI BanI BanII BbeI
BbrPI BbvCI BcgI BciVI BclI
BcnI BfaI BfrI BglI BglII
BlnI BlpI BmyI BpmI Bpu1102I
BsaAI BsaBI BsaHI BsaI BsaJI
BsaWI BseAI BseRI BsiEI BsiHKAI
BsiWI BsmAI BsmBI BsmFI BsmI
Bsp1286I Bsp1407I BspDI BspEI BspHI
BspLU11I BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstUI BstXI
BstYI BstZ17I Bsu36I CelII CfoI
Cfr10I ClaI CpoI DdeI DpnII
DraI DraIII DrdI DsaI EaeI
EagI Eam1105I EarI EclXI Eco47III
Eco52I Eco81I EcoO65I EcoRI EcoRV
EcoT14I EcoT22I FauI FbaI FseI
FspI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HincII HindII
HindIII HpaI HpaII HphI KasI
KpnI Ksp632I KspI MaeI MaeII
MamI MboI MfeI MflI MluI
MluNI MroI MscI MseI MspA1I
MspI MunI MvnI MwoI NaeI
NarI NciI NcoI NdeI NdeII
NgoMIV NheI NotI NruI NsiI
NspI NspV PacI PinAI PmaCI
PmeI PmlI PshBI Psp1406I PstI
PvuI PvuII RcaI RsaI RsrII
SacI SacII SalI SapI Sau3AI
SbfI ScaI SexAI SfcI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI StyI SwaI TfiI Tru9I
Tsp45I XbaI XcmI XhoII XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 39 enzymes) #
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AspI 142 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 161 CTCGAG C^[CT]CG[GA]G
AvaII 193 GGACC G^G[AT]CC
BbvI 206 GCTGC GCAGC(8/12)
BsgI 53 GTGCAG GTGCAG(16/14)
BsoBI 161 CTCGAG C^[CT]CG[GA]G
BsrI 184 ACTGG ACTGG(1/-1)
BssKI 108 CCAGG ^CC[ACGT]GG
BstF5I 93 GGATG GGATG(2/0)
BstNI 108 CCAGG CC^[AT]GG
Cac8I 55 GCAGGC GC[ACGT]^[ACGT]GC
Cfr13I 193 GGACC G^G[ACGT]CC
DraII 192 AGGACCC [GA]G^G[ACGT]CC[CT]
EcoNI 84 CCTATTAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 192 AGGACCC [GA]G^G[ACGT]CC[CT]
EcoRII 108 CCAGG ^CC[AT]GG
Fnu4HI 206 GCTGC GC^[ACGT]GC
FokI 93 GGATG GGATG(9/13)
HinfI 164 GAGTC G^A[ACGT]TC
ItaI 206 GCTGC GC^[ACGT]GC
MaeIII 181 GTTAC ^GT[ACGT]AC
MvaI 108 CCAGG CC^[AT]GG
NlaIV 193 GGACCC GG[ACGT]^[ACGT]CC
PaeR7I 161 CTCGAG C^TCGAG
PflFI 142 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 42 CCATTCAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 164 GAGTC GAGTC(4/5)
PpuMI 192 AGGACCC [GA]G^G[AT]CC[CT]
PshAI 194 GACCCAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Sau96I 193 GGACC G^G[ACGT]CC
ScrFI 108 CCAGG CC^[ACGT]GG
SfaNI 222 GCATC GCATC(5/9)
SmlI 161 CTCGAG C^T[CT][GA]AG
TaqI 162 TCGA T^CGA
TseI 206 GCTGC G^C[AT]GC
Tth111I 142 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 162 TCGA T^CGA
Van91I 42 CCATTCAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 161 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 10 enzymes) #
###################################
AcsI 32 GAATTT [GA]^AATT[CT]
211 AAATTT
ApoI 32 GAATTT [GA]^AATT[CT]
211 AAATTT
BbsI 112 GAAGAC GAAGAC(2/6)
235 GAAGAC
BpuAI 112 GAAGAC GAAGAC(2/6)
235 GAAGAC
BsiYI 42 CCATTCAGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
84 CCTATTAGAGG
BslI 42 CCATTCAGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
84 CCTATTAGAGG
MslI 144 CACTGTCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
223 CATCTCTGTG
NlaIII 117 CATG CATG^
150 CATG
Tsp509I 33 AATT ^AATT
212 AATT
TspRI 47 CAGTG CA[GC]TG[ACGT][ACGT]^
144 CACTG
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# 3 OR MORE CUT ENZYMES ( 2 enzymes) #
#########################################
MboII MnlI