################################## # NO CUT ENZYMES ( 185 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcsI AcyI AflII AflIII AgeI AhdI AluI Alw44I AlwNI AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvaI AviII BalI BamHI BanI BanII BbeI BbrPI BbvCI BcgI BciVI BclI BfrI BglI BlpI BpmI Bpu1102I BsaAI BsaBI BsaHI BsaWI BseAI BsgI BsiEI BsiWI BsmBI BsmI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstF5I BstPI BstXI BstZ17I Bsu36I Cac8I CelII CfoI Cfr10I ClaI CpoI DraI DraIII EaeI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT22I FbaI FokI FseI FspI HaeII HhaI Hin1I HinP1I HincII HindII HindIII HpaI KasI KpnI Ksp632I KspI MaeII MamI MfeI MluI MluNI MroI MscI MseI MslI MunI MwoI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PleI PmaCI PmeI PmlI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfaNI SfcI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI TaqI Tru9I Tth111I TthHB8I Van91I XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 47 enzymes) # ################################### AccII 134 CGCG CG^CG AfaI 224 GTAC GT^AC AlwI 129 GATCC GGATC(4/5) AspHI 174 GTGCTC G[AT]GC[AT]^C AvrII 51 CCTAGG C^CTAGG BbsI 193 GTCTTC GAAGAC(2/6) BcnI 184 CCCGG CC^[GC]GG BfaI 52 CTAG C^TAG BglII 5 AGATCT A^GATCT BlnI 51 CCTAGG C^CTAGG BmyI 174 GTGCTC G[AGT]GC[ACT]^C BpuAI 193 GTCTTC GAAGAC(2/6) BsaI 160 GAGACC GGTCTC(1/5) BsiHKAI 174 GTGCTC G[AT]GC[AT]^C BsiYI 71 CCTCCTCACGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BslI 71 CCTCCTCACGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BsmAI 160 GAGAC GTCTC(1/5) Bsp1286I 174 GTGCTC G[AGT]GC[ACT]^C BstNI 249 CCAGG CC^[AT]GG BstUI 134 CGCG CG^CG DdeI 96 CTCAG C^T[ACGT]AG DraII 81 GGGACCT [GA]G^G[ACGT]CC[CT] DrdI 238 GACAAGTGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC DsaI 11 CCGTGG C^C[GA][CT]GG EcoO109I 81 GGGACCT [GA]G^G[ACGT]CC[CT] EcoRII 249 CCAGG ^CC[AT]GG EcoT14I 51 CCTAGG C^C[AT][AT]GG FauI 132 CCCGC CCCGC(4/6) HaeIII 211 GGCC GG^CC HapII 185 CCGG C^CGG HgaI 135 GCGTC GACGC(5/10) HinfI 67 GAATC G^A[ACGT]TC HpaII 185 CCGG C^CGG MaeI 52 CTAG C^TAG MaeIII 236 GTGAC ^GT[ACGT]AC MspA1I 139 CAGCGG C[AC]G^C[GT]G MspI 185 CCGG C^CGG MvaI 249 CCAGG CC^[AT]GG MvnI 134 CGCG CG^CG NciI 184 CCCGG CC^[GC]GG NlaIII 267 CATG CATG^ PpuMI 81 GGGACCT [GA]G^G[AT]CC[CT] RsaI 224 GTAC GT^AC StyI 51 CCTAGG C^C[AT][AT]GG TfiI 67 GAATC G^A[AT]TC Tsp45I 236 GTGAC ^GT[GC]AC Tsp509I 189 AATT ^AATT ################################### # TWO CUT ENZYMES ( 19 enzymes) # ################################### AciI 133 CCGC CCGC(-3/-1) 141 GCGG BbvI 16 GCTGC GCAGC(8/12) 104 GCTGC BsaJI 11 CCGTGG C^C[ACGT][ACGT]GG 51 CCTAGG BseRI 34 CTCCTC GAGGAG(10/8) 72 CTCCTC BsmFI 55 GGGAC GGGAC(10/14) 81 GGGAC BsrI 171 CCAGT ACTGG(1/-1) 233 CCAGT BssKI 184 CCCGG ^CC[ACGT]GG 249 CCAGG BstYI 5 AGATCT [GA]^GATC[CT] 128 AGATCC DpnII 6 GATC ^GATC 129 GATC Fnu4HI 16 GCTGC GC^[ACGT]GC 104 GCTGC ItaI 16 GCTGC GC^[ACGT]GC 104 GCTGC MboI 6 GATC ^GATC 129 GATC MflI 5 AGATCT [GA]^GATC[CT] 128 AGATCC NdeII 6 GATC ^GATC 129 GATC Sau3AI 6 GATC ^GATC 129 GATC ScrFI 184 CCCGG CC^[ACGT]GG 249 CCAGG TseI 16 GCTGC G^C[AT]GC 104 GCTGC TspRI 172 CAGTG CA[GC]TG[ACGT][ACGT]^ 234 CAGTG XhoII 5 AGATCT [GA]^GATC[CT] 128 AGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 7 enzymes) # ######################################### AvaII Cfr13I HphI MboII MnlI NlaIV Sau96I