Restriction map for pF1KB0905
##################################
# NO CUT ENZYMES (  185 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AcsI        AcyI        AflII       AflIII      AgeI        
AhdI        AluI        Alw44I      AlwNI       AocI        
Aor51HI     ApaI        ApaLI       ApoI        AscI        
AseI        AsnI        Asp700I     Asp718I     AspEI       
AspI        AvaI        AviII       BalI        BamHI       
BanI        BanII       BbeI        BbrPI       BbvCI       
BcgI        BciVI       BclI        BfrI        BglI        
BlpI        BpmI        Bpu1102I    BsaAI       BsaBI       
BsaHI       BsaWI       BseAI       BsgI        BsiEI       
BsiWI       BsmBI       BsmI        BsoBI       Bsp1407I    
BspDI       BspEI       BspHI       BspLU11I    BspMI       
BsrBI       BsrDI       BsrFI       BsrGI       BssHII      
BssSI       Bst1107I    BstAPI      BstBI       BstEII      
BstF5I      BstPI       BstXI       BstZ17I     Bsu36I      
Cac8I       CelII       CfoI        Cfr10I      ClaI        
CpoI        DraI        DraIII      EaeI        EagI        
Eam1105I    EarI        EclXI       Eco47III    Eco52I      
Eco81I      EcoNI       EcoO65I     EcoRI       EcoRV       
EcoT22I     FbaI        FokI        FseI        FspI        
HaeII       HhaI        Hin1I       HinP1I      HincII      
HindII      HindIII     HpaI        KasI        KpnI        
Ksp632I     KspI        MaeII       MamI        MfeI        
MluI        MluNI       MroI        MscI        MseI        
MslI        MunI        MwoI        NaeI        NarI        
NcoI        NdeI        NgoMIV      NheI        NotI        
NruI        NsiI        NspI        NspV        PacI        
PaeR7I      PflFI       PflMI       PinAI       PleI        
PmaCI       PmeI        PmlI        PshAI       PshBI       
Psp1406I    PstI        PvuI        PvuII       RcaI        
RsrII       SacI        SacII       SalI        SapI        
SbfI        ScaI        SexAI       SfaNI       SfcI        
SfiI        SfoI        SfuI        SgrAI       SmaI        
SmlI        SnaBI       SpeI        SphI        Sse8387I    
SspBI       SspI        StuI        SwaI        TaqI        
Tru9I       Tth111I     TthHB8I     Van91I      XbaI        
XcmI        XhoI        XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   47 enzymes) #
###################################

AccII         134  CGCG                 CG^CG
AfaI          224  GTAC                 GT^AC
AlwI          129  GATCC                GGATC(4/5)
AspHI         174  GTGCTC               G[AT]GC[AT]^C
AvrII          51  CCTAGG               C^CTAGG
BbsI          193  GTCTTC               GAAGAC(2/6)
BcnI          184  CCCGG                CC^[GC]GG
BfaI           52  CTAG                 C^TAG
BglII           5  AGATCT               A^GATCT
BlnI           51  CCTAGG               C^CTAGG
BmyI          174  GTGCTC               G[AGT]GC[ACT]^C
BpuAI         193  GTCTTC               GAAGAC(2/6)
BsaI          160  GAGACC               GGTCTC(1/5)
BsiHKAI       174  GTGCTC               G[AT]GC[AT]^C
BsiYI          71  CCTCCTCACGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI           71  CCTCCTCACGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmAI         160  GAGAC                GTCTC(1/5)
Bsp1286I      174  GTGCTC               G[AGT]GC[ACT]^C
BstNI         249  CCAGG                CC^[AT]GG
BstUI         134  CGCG                 CG^CG
DdeI           96  CTCAG                C^T[ACGT]AG
DraII          81  GGGACCT              [GA]G^G[ACGT]CC[CT]
DrdI          238  GACAAGTGGGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI           11  CCGTGG               C^C[GA][CT]GG
EcoO109I       81  GGGACCT              [GA]G^G[ACGT]CC[CT]
EcoRII        249  CCAGG                ^CC[AT]GG
EcoT14I        51  CCTAGG               C^C[AT][AT]GG
FauI          132  CCCGC                CCCGC(4/6)
HaeIII        211  GGCC                 GG^CC
HapII         185  CCGG                 C^CGG
HgaI          135  GCGTC                GACGC(5/10)
HinfI          67  GAATC                G^A[ACGT]TC
HpaII         185  CCGG                 C^CGG
MaeI           52  CTAG                 C^TAG
MaeIII        236  GTGAC                ^GT[ACGT]AC
MspA1I        139  CAGCGG               C[AC]G^C[GT]G
MspI          185  CCGG                 C^CGG
MvaI          249  CCAGG                CC^[AT]GG
MvnI          134  CGCG                 CG^CG
NciI          184  CCCGG                CC^[GC]GG
NlaIII        267  CATG                 CATG^
PpuMI          81  GGGACCT              [GA]G^G[AT]CC[CT]
RsaI          224  GTAC                 GT^AC
StyI           51  CCTAGG               C^C[AT][AT]GG
TfiI           67  GAATC                G^A[AT]TC
Tsp45I        236  GTGAC                ^GT[GC]AC
Tsp509I       189  AATT                 ^AATT


###################################
# TWO CUT ENZYMES (   19 enzymes) #
###################################

AciI          133  CCGC                 CCGC(-3/-1)
              141  GCGG                
BbvI           16  GCTGC                GCAGC(8/12)
              104  GCTGC               
BsaJI          11  CCGTGG               C^C[ACGT][ACGT]GG
               51  CCTAGG              
BseRI          34  CTCCTC               GAGGAG(10/8)
               72  CTCCTC              
BsmFI          55  GGGAC                GGGAC(10/14)
               81  GGGAC               
BsrI          171  CCAGT                ACTGG(1/-1)
              233  CCAGT               
BssKI         184  CCCGG                ^CC[ACGT]GG
              249  CCAGG               
BstYI           5  AGATCT               [GA]^GATC[CT]
              128  AGATCC              
DpnII           6  GATC                 ^GATC
              129  GATC                
Fnu4HI         16  GCTGC                GC^[ACGT]GC
              104  GCTGC               
ItaI           16  GCTGC                GC^[ACGT]GC
              104  GCTGC               
MboI            6  GATC                 ^GATC
              129  GATC                
MflI            5  AGATCT               [GA]^GATC[CT]
              128  AGATCC              
NdeII           6  GATC                 ^GATC
              129  GATC                
Sau3AI          6  GATC                 ^GATC
              129  GATC                
ScrFI         184  CCCGG                CC^[ACGT]GG
              249  CCAGG               
TseI           16  GCTGC                G^C[AT]GC
              104  GCTGC               
TspRI         172  CAGTG                CA[GC]TG[ACGT][ACGT]^
              234  CAGTG               
XhoII           5  AGATCT               [GA]^GATC[CT]
              128  AGATCC              


#########################################
# 3 OR MORE CUT ENZYMES (    7 enzymes) #
#########################################

AvaII       Cfr13I      HphI        MboII       MnlI        
NlaIV       Sau96I      
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