##################################
# NO CUT ENZYMES (  218 enzymes) #
##################################

AatII       AccI        AccII       AccIII      AciI        
AclI        AcsI        AcyI        AflIII      AgeI        
AhdI        Alw44I      AlwI        AlwNI       AocI        
Aor51HI     ApaI        ApaLI       ApoI        AscI        
AseI        AsnI        Asp700I     AspEI       AspHI       
AspI        AvaI        AvaII       AviII       AvrII       
BalI        BamHI       BbeI        BbrPI       BbsI        
BbvCI       BcgI        BciVI       BclI        BcnI        
BfaI        BglI        BglII       BlnI        BlpI        
BpmI        Bpu1102I    BpuAI       BsaAI       BsaBI       
BsaHI       BsaI        BsaWI       BseAI       BseRI       
BsgI        BsiEI       BsiHKAI     BsiWI       BsiYI       
BslI        BsmBI       BsmFI       BsmI        BsoBI       
Bsp1407I    BspDI       BspEI       BspHI       BspLU11I    
BspMI       BsrBI       BsrDI       BsrFI       BsrGI       
BsrI        BssHII      BssSI       Bst1107I    BstAPI      
BstBI       BstEII      BstF5I      BstPI       BstUI       
BstXI       BstYI       BstZ17I     Bsu36I      CelII       
CfoI        Cfr10I      ClaI        CpoI        DpnII       
DraII       DraIII      DrdI        DsaI        EaeI        
EagI        Eam1105I    EarI        EclXI       Eco47III    
Eco52I      Eco81I      EcoNI       EcoO109I    EcoO65I     
EcoRI       EcoRV       EcoT14I     EcoT22I     FauI        
FbaI        FokI        FseI        FspI        HaeII       
HapII       HgaI        HhaI        Hin1I       HinP1I      
HincII      HindII      HinfI       HpaI        HpaII       
HphI        KasI        Ksp632I     KspI        MaeI        
MaeIII      MamI        MboI        MboII       MfeI        
MflI        MluI        MluNI       MroI        MscI        
MslI        MspA1I      MspI        MunI        MvnI        
NaeI        NarI        NciI        NcoI        NdeI        
NdeII       NgoMIV      NheI        NotI        NruI        
NsiI        NspI        NspV        PacI        PaeR7I      
PflFI       PflMI       PinAI       PleI        PmaCI       
PmeI        PmlI        PpuMI       PshAI       PshBI       
Psp1406I    PstI        PvuI        PvuII       RcaI        
RsrII       SacI        SacII       SalI        SapI        
Sau3AI      SbfI        ScaI        SexAI       SfcI        
SfiI        SfoI        SfuI        SgrAI       SmaI        
SnaBI       SpeI        SphI        Sse8387I    SspBI       
SspI        StuI        StyI        SwaI        TaqI        
TfiI        Tsp45I      Tsp509I     Tth111I     TthHB8I     
Van91I      XbaI        XcmI        XhoI        XhoII       
XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   25 enzymes) #
###################################

Acc65I        146  GGTACC               G^GTACC
AflII         162  CTTAAG               C^TTAAG
AluI          191  AGCT                 AG^CT
Asp718I       146  GGTACC               G^GTACC
BanI          146  GGTACC               G^G[CT][GA]CC
BanII         222  GAGCCC               G[GA]GC[CT]^C
BbvI          204  GCTGC                GCAGC(8/12)
BfrI          162  CTTAAG               C^TTAAG
BmyI          222  GAGCCC               G[AGT]GC[ACT]^C
BsaJI         225  CCCAGG               C^C[ACGT][ACGT]GG
Bsp1286I      222  GAGCCC               G[AGT]GC[ACT]^C
Cac8I         273  GCTTGC               GC[ACGT]^[ACGT]GC
Cfr13I          3  GGCCC                G^G[ACGT]CC
Fnu4HI        204  GCTGC                GC^[ACGT]GC
HaeIII          3  GGCC                 GG^CC
HindIII       190  AAGCTT               A^AGCTT
ItaI          204  GCTGC                GC^[ACGT]GC
KpnI          146  GGTACC               GGTAC^C
MaeII          44  ACGT                 A^CGT
MnlI          263  CCTC                 CCTC(7/6)
MwoI          175  GCGAACCAAGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
Sau96I          3  GGCCC                G^G[ACGT]CC
SfaNI         201  GATGC                GCATC(5/9)
TseI          204  GCTGC                G^C[AT]GC
TspRI         252  CAGTG                CA[GC]TG[ACGT][ACGT]^


###################################
# TWO CUT ENZYMES (   12 enzymes) #
###################################

AfaI          147  GTAC                 GT^AC
              230  GTAC                
BsmAI         168  GAGAC                GTCTC(1/5)
              248  GAGAC               
BssKI         143  CCTGG                ^CC[ACGT]GG
              226  CCAGG               
BstNI         143  CCTGG                CC^[AT]GG
              226  CCAGG               
DraI           15  TTTAAA               TTT^AAA
               84  TTTAAA              
EcoRII        143  CCTGG                ^CC[AT]GG
              226  CCAGG               
MvaI          143  CCTGG                CC^[AT]GG
              226  CCAGG               
NlaIII        198  CATG                 CATG^
              244  CATG                
NlaIV         106  GGTTCC               GG[ACGT]^[ACGT]CC
              146  GGTACC              
RsaI          147  GTAC                 GT^AC
              230  GTAC                
ScrFI         143  CCTGG                CC^[ACGT]GG
              226  CCAGG               
SmlI           99  CTCAAG               C^T[CT][GA]AG
              162  CTTAAG              


#########################################
# 3 OR MORE CUT ENZYMES (    3 enzymes) #
#########################################

DdeI        MseI        Tru9I