Restriction map for
pF1KB0908
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# NO CUT ENZYMES ( 149 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcsI AcyI AflII AflIII AgeI
AhdI Alw44I AocI ApaI ApaLI
ApoI AscI AseI AsnI Asp700I
Asp718I AspEI AspHI AspI AvaI
AvrII BalI BanI BanII BbeI
BbrPI BbsI BbvCI BciVI BfrI
BglII BlnI BmyI BpuAI BsaAI
BsaBI BsaHI BsaI BsaWI BseAI
BsiEI BsiHKAI BsiWI BsmI BsoBI
Bsp1286I Bsp1407I BspDI BspEI BspLU11I
BsrBI BsrGI BsrI BssHII BssSI
Bst1107I BstAPI BstBI BstUI BstXI
BstZ17I Bsu36I ClaI CpoI DraIII
DrdI DsaI EaeI EagI Eam1105I
EclXI Eco52I Eco81I EcoNI EcoRI
EcoRV EcoT22I FseI Hin1I HinfI
HpaI KasI KpnI KspI MamI
MfeI MluI MluNI MroI MscI
MunI MvnI NarI NcoI NdeI
NheI NotI NruI NsiI NspI
NspV PacI PaeR7I PflFI PflMI
PinAI PleI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI RsrII
SacI SacII SalI SapI SbfI
ScaI SfiI SfoI SfuI SgrAI
SmaI SmlI SnaBI SpeI SphI
Sse8387I SspBI SspI SwaI TfiI
Tsp509I Tth111I Van91I XbaI XcmI
XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 49 enzymes) #
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AfaI 354 GTAC GT^AC
AlwNI 64 CAGGCGCTG CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI 845 AGCGCT AGC^GCT
AviII 159 TGCGCA TGC^GCA
BamHI 282 GGATCC G^GATCC
BcgI 86 GCAGGGTGTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 494 TGATCA T^GATCA
BfaI 367 CTAG C^TAG
BglI 58 GCCATCCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 448 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 448 GCTCAGC GC^T[ACGT]AGC
BseRI 302 CTCCTC GAGGAG(10/8)
BsgI 289 GTGCAG GTGCAG(16/14)
BsmAI 127 GAGAC GTCTC(1/5)
BsmBI 127 GAGACG CGTCTC(1/5)
BsmFI 194 GGGAC GGGAC(10/14)
BspHI 797 TCATGA T^CATGA
BspMI 922 ACCTGC ACCTGC(4/8)
BsrDI 562 GCAATG GCAATG(2/0)
BsrFI 926 GCCGGC [GA]^CCGG[CT]
BstEII 573 GGTGACC G^GT[ACGT]ACC
BstPI 573 GGTGACC G^GT[ACGT]ACC
BstYI 282 GGATCC [GA]^GATC[CT]
CelII 448 GCTCAGC GC^T[ACGT]AGC
Cfr10I 926 GCCGGC [GA]^CCGG[CT]
DraI 381 TTTAAA TTT^AAA
EarI 254 CTCTTC CTCTTC(1/4)
Eco47III 845 AGCGCT AGC^GCT
EcoO65I 573 GGTGACC G^GT[ACGT]ACC
EcoT14I 578 CCTTGG C^C[AT][AT]GG
FauI 851 GCGGG CCCGC(4/6)
FbaI 494 TGATCA T^GATCA
FspI 159 TGCGCA TGC^GCA
HgaI 129 GACGC GACGC(5/10)
HindIII 679 AAGCTT A^AGCTT
Ksp632I 254 CTCTTC CTCTTC(1/4)
MaeI 367 CTAG C^TAG
MflI 282 GGATCC [GA]^GATC[CT]
MspA1I 760 CAGCTG C[AC]G^C[GT]G
NaeI 926 GCCGGC GCC^GGC
NgoMIV 926 GCCGGC G^CCGGC
PpuMI 194 GGGACCT [GA]G^G[AT]CC[CT]
PstI 862 CTGCAG CTGCA^G
PvuII 760 CAGCTG CAG^CTG
RcaI 797 TCATGA T^CATGA
RsaI 354 GTAC GT^AC
SexAI 802 ACCTGGT A^CC[AT]GGT
StyI 578 CCTTGG C^C[AT][AT]GG
XhoII 282 GGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 15 enzymes) #
###################################
AccI 437 GTAGAC GT^[AC][GT]AC
661 GTCTAC
BcnI 141 CCGGG CC^[GC]GG
192 CCGGG
BsaJI 44 CCCAGG C^C[ACGT][ACGT]GG
578 CCTTGG
DraII 194 GGGACCT [GA]G^G[ACGT]CC[CT]
833 AGGCCCC
EcoO109I 194 GGGACCT [GA]G^G[ACGT]CC[CT]
833 AGGCCCC
HaeII 66 GGCGCT [GA]GCGC^[CT]
845 AGCGCT
HincII 187 GTCAAC GT[CT]^[GA]AC
314 GTCAAC
HindII 187 GTCAAC GT[CT]^[GA]AC
314 GTCAAC
HphI 573 GGTGA GGTGA(8/7)
723 GGTGA
MboII 670 GAAGA GAAGA(8/7)
255 TCTTC
MslI 259 CATAACAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
329 CATATAAGTG
NciI 141 CCGGG CC^[GC]GG
192 CCGGG
SfcI 309 CTATAG C^T[GA][CT]AG
862 CTGCAG
StuI 35 AGGCCT AGG^CCT
149 AGGCCT
Tsp45I 574 GTGAC ^GT[GC]AC
724 GTGAC
#########################################
# 3 OR MORE CUT ENZYMES ( 45 enzymes) #
#########################################
AciI AluI AlwI AvaII BbvI
BpmI BsiYI BslI BssKI BstF5I
BstNI Cac8I CfoI Cfr13I DdeI
DpnII EcoRII Fnu4HI FokI HaeIII
HapII HhaI HinP1I HpaII ItaI
MaeII MaeIII MboI MnlI MseI
MspI MvaI MwoI NdeII NlaIII
NlaIV Sau3AI Sau96I ScrFI SfaNI
TaqI Tru9I TseI TspRI TthHB8I