##################################
# NO CUT ENZYMES (  187 enzymes) #
##################################

AatII       Acc65I      AccII       AccIII      AclI        
AcsI        AcyI        AflII       AflIII      AgeI        
AhdI        Alw44I      AlwI        AlwNI       AocI        
Aor51HI     ApaLI       ApoI        AscI        Asp700I     
Asp718I     AspEI       AspI        AvaI        AviII       
AvrII       BalI        BamHI       BbeI        BbrPI       
BbsI        BbvCI       BcgI        BclI        BfaI        
BfrI        BglI        BglII       BlnI        BlpI        
Bpu1102I    BpuAI       BsaAI       BsaBI       BsaHI       
BsaI        BsaWI       BseAI       BsgI        BsiEI       
BsiWI       BsmAI       BsmBI       BsoBI       Bsp1407I    
BspDI       BspEI       BspHI       BspLU11I    BspMI       
BsrBI       BsrDI       BsrFI       BsrGI       BsrI        
BssHII      BssSI       Bst1107I    BstAPI      BstBI       
BstEII      BstPI       BstUI       BstYI       BstZ17I     
Bsu36I      CelII       CfoI        Cfr10I      ClaI        
CpoI        DdeI        DpnII       DraI        DraIII      
DrdI        EaeI        EagI        Eam1105I    EclXI       
Eco47III    Eco52I      Eco81I      EcoO65I     EcoRI       
EcoRV       EcoT22I     FauI        FbaI        FseI        
FspI        HaeII       HgaI        HhaI        Hin1I       
HinP1I      HincII      HindII      HindIII     HpaI        
HphI        KasI        KpnI        KspI        MaeI        
MaeII       MaeIII      MamI        MboI        MfeI        
MflI        MluI        MluNI       MroI        MscI        
MspA1I      MunI        MvnI        NaeI        NarI        
NdeI        NdeII       NgoMIV      NheI        NotI        
NruI        NsiI        NspI        NspV        PacI        
PaeR7I      PflFI       PinAI       PleI        PmaCI       
PmeI        PmlI        PshAI       Psp1406I    PstI        
PvuI        PvuII       RcaI        RsrII       SacII       
SalI        SapI        Sau3AI      SbfI        ScaI        
SexAI       SfcI        SfiI        SfoI        SfuI        
SgrAI       SmaI        SmlI        SnaBI       SpeI        
SphI        Sse8387I    SspBI       StuI        SwaI        
TaqI        Tsp45I      Tsp509I     Tth111I     TthHB8I     
XbaI        XcmI        XhoI        XhoII       XmaCI       
XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   36 enzymes) #
###################################

AccI          313  GTAGAC               GT^[AC][GT]AC
AciI          247  CCGC                 CCGC(-3/-1)
AfaI          384  GTAC                 GT^AC
ApaI          172  GGGCCC               GGGCC^C
AseI           94  ATTAAT               AT^TAAT
AsnI           94  ATTAAT               AT^TAAT
AspHI         137  GAGCTC               G[AT]GC[AT]^C
BanI           56  GGTGCC               G^G[CT][GA]CC
BbvI           21  GCAGC                GCAGC(8/12)
BciVI         201  GTATCC               GTATCC(6/5)
BcnI           17  CCGGG                CC^[GC]GG
BpmI           62  CTGGAG               CTGGAG(16/14)
BsiHKAI       137  GAGCTC               G[AT]GC[AT]^C
BsmFI         224  GTCCC                GGGAC(10/14)
BsmI          402  GCATTC               GAATGC(1/-1)
BstXI         387  CCACAAGCATGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DsaI          144  CCATGG               C^C[GA][CT]GG
EcoNI         127  CCTCCCCCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
Fnu4HI         21  GCAGC                GC^[ACGT]GC
HapII          17  CCGG                 C^CGG
HpaII          17  CCGG                 C^CGG
ItaI           21  GCAGC                GC^[ACGT]GC
MseI           95  TTAA                 T^TAA
MslI          388  CACAAGCATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI           17  CCGG                 C^CGG
NciI           17  CCGGG                CC^[GC]GG
NcoI          144  CCATGG               C^CATGG
PflMI         213  CCAACCGTTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI         222  GGGTCCC              [GA]G^G[AT]CC[CT]
PshBI          94  ATTAAT               AT^TAAT
RsaI          384  GTAC                 GT^AC
SacI          137  GAGCTC               GAGCT^C
SspI           49  AATATT               AAT^ATT
Tru9I          95  TTAA                 T^TAA
TseI           21  GCAGC                G^C[AT]GC
Van91I        213  CCAACCGTTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG


###################################
# TWO CUT ENZYMES (   10 enzymes) #
###################################

AvaII         223  GGTCC                G^G[AT]CC
              298  GGACC               
BanII         137  GAGCTC               G[GA]GC[CT]^C
              172  GGGCCC              
BstF5I        181  CATCC                GGATG(2/0)
              208  GGATG               
Cac8I         249  GCCTGC               GC[ACGT]^[ACGT]GC
              398  GCAAGC              
EarI          411  CTCTTC               CTCTTC(1/4)
              423  CTCTTC              
EcoT14I       144  CCATGG               C^C[AT][AT]GG
              278  CCTTGG              
FokI          181  CATCC                GGATG(9/13)
              208  GGATG               
Ksp632I       411  CTCTTC               CTCTTC(1/4)
              423  CTCTTC              
StyI          144  CCATGG               C^C[AT][AT]GG
              278  CCTTGG              
TspRI         305  CAGTG                CA[GC]TG[ACGT][ACGT]^
              432  CAGTG               


#########################################
# 3 OR MORE CUT ENZYMES (   25 enzymes) #
#########################################

AluI        BmyI        BsaJI       BseRI       BsiYI       
BslI        Bsp1286I    BssKI       BstNI       Cfr13I      
DraII       EcoO109I    EcoRII      HaeIII      HinfI       
MboII       MnlI        MvaI        MwoI        NlaIII      
NlaIV       Sau96I      ScrFI       SfaNI       TfiI