################################## # NO CUT ENZYMES ( 147 enzymes) # ################################## Acc65I AccI AccII AccIII AclI AcsI AflII AgeI AhdI Alw44I AocI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvaI AvrII BamHI BanII BbeI BbrPI BcgI BciVI BclI BcnI BfrI BglI BlnI BlpI Bpu1102I BsaBI BsaI BsaWI BseAI BsgI BsiWI BsmBI BsmFI BsmI BsoBI Bsp1407I BspDI BspEI BsrBI BsrDI BsrFI BsrGI BssHII Bst1107I BstBI BstEII BstPI BstUI BstXI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DraI DraII DrdI DsaI EagI Eam1105I EclXI Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRV EcoT14I EcoT22I FbaI FseI HindIII HpaI KasI KpnI KspI MamI MluI MroI MslI MvnI NaeI NarI NciI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StyI SwaI TfiI Tth111I XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 39 enzymes) # ################################### AatII 462 GACGTC GACGT^C AcyI 462 GACGTC G[GA]^CG[CT]C Aor51HI 689 AGCGCT AGC^GCT AvaII 165 GGACC G^G[AT]CC AviII 930 TGCGCA TGC^GCA BanI 90 GGCACC G^G[CT][GA]CC BbsI 459 GAAGAC GAAGAC(2/6) BbvCI 306 CCTCAGC CCTCAGC(-5/-2) BglII 42 AGATCT A^GATCT BpuAI 459 GAAGAC GAAGAC(2/6) BsaAI 823 TACGTG [CT]AC^GT[GA] BsaHI 462 GACGTC G[GA]^CG[CT]C BsiEI 182 CGGTCG CG[GA][CT]^CG BspHI 920 TCATGA T^CATGA BspMI 410 ACCTGC ACCTGC(4/8) BssSI 751 CACGAG CACGAG(-5/-1) BstAPI 116 GCAACGCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC DraIII 129 CACCCAGTG CAC[ACGT][ACGT][ACGT]^GTG EarI 943 GAAGAG CTCTTC(1/4) Eco47III 689 AGCGCT AGC^GCT FauI 635 CCCGC CCCGC(4/6) FspI 930 TGCGCA TGC^GCA HaeII 689 AGCGCT [GA]GCGC^[CT] HapII 282 CCGG C^CGG HgaI 806 GACGC GACGC(5/10) Hin1I 462 GACGTC G[GA]^CG[CT]C HpaII 282 CCGG C^CGG Ksp632I 943 GAAGAG CTCTTC(1/4) MfeI 578 CAATTG C^AATTG MseI 404 TTAA T^TAA MspI 282 CCGG C^CGG MunI 578 CAATTG C^AATTG PstI 235 CTGCAG CTGCA^G RcaI 920 TCATGA T^CATGA StuI 912 AGGCCT AGG^CCT TaqI 655 TCGA T^CGA Tru9I 404 TTAA T^TAA Tsp509I 579 AATT ^AATT TthHB8I 655 TCGA T^CGA ################################### # TWO CUT ENZYMES ( 23 enzymes) # ################################### AflIII 154 ACATGT A^C[GA][CT]GT 221 ACATGT AlwI 594 GATCC GGATC(4/5) 209 GGATC AspHI 319 GAGCAC G[AT]GC[AT]^C 670 GTGCTC BalI 776 TGGCCA TGG^CCA 827 TGGCCA BmyI 319 GAGCAC G[AGT]GC[ACT]^C 670 GTGCTC BseRI 76 GAGGAG GAGGAG(10/8) 571 GAGGAG BsiHKAI 319 GAGCAC G[AT]GC[AT]^C 670 GTGCTC Bsp1286I 319 GAGCAC G[AGT]GC[ACT]^C 670 GTGCTC BspLU11I 154 ACATGT A^CATGT 221 ACATGT BstYI 42 AGATCT [GA]^GATC[CT] 593 AGATCC Cfr13I 165 GGACC G^G[ACGT]CC 764 GGCCC EaeI 776 TGGCCA [CT]^GGCC[GA] 827 TGGCCA HinfI 68 GAGTC G^A[ACGT]TC 657 GACTC MaeII 463 ACGT A^CGT 824 ACGT MflI 42 AGATCT [GA]^GATC[CT] 593 AGATCC MluNI 776 TGGCCA TGG^CCA 827 TGGCCA MscI 776 TGGCCA TGG^CCA 827 TGGCCA PflMI 768 CCAGGGTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 879 CCAGCACATGG PleI 657 GACTC GAGTC(4/5) 68 GAGTC Sau96I 165 GGACC G^G[ACGT]CC 764 GGCCC SfcI 235 CTGCAG C^T[GA][CT]AG 267 CTGTAG Van91I 768 CCAGGGTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 879 CCAGCACATGG XhoII 42 AGATCT [GA]^GATC[CT] 593 AGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 49 enzymes) # ######################################### AciI AfaI AluI AlwNI BbvI BfaI BpmI BsaJI BsiYI BslI BsmAI BsrI BssKI BstF5I BstNI Cac8I CfoI DdeI DpnII EcoRII Fnu4HI FokI HaeIII HhaI HinP1I HincII HindII HphI ItaI MaeI MaeIII MboI MboII MnlI MspA1I MvaI MwoI NdeII NlaIII NlaIV NspI PvuII RsaI Sau3AI ScrFI SfaNI TseI Tsp45I TspRI