Restriction map for pF1KB0912
##################################
# NO CUT ENZYMES (  147 enzymes) #
##################################

Acc65I      AccI        AccII       AccIII      AclI        
AcsI        AflII       AgeI        AhdI        Alw44I      
AocI        ApaI        ApaLI       ApoI        AscI        
AseI        AsnI        Asp700I     Asp718I     AspEI       
AspI        AvaI        AvrII       BamHI       BanII       
BbeI        BbrPI       BcgI        BciVI       BclI        
BcnI        BfrI        BglI        BlnI        BlpI        
Bpu1102I    BsaBI       BsaI        BsaWI       BseAI       
BsgI        BsiWI       BsmBI       BsmFI       BsmI        
BsoBI       Bsp1407I    BspDI       BspEI       BsrBI       
BsrDI       BsrFI       BsrGI       BssHII      Bst1107I    
BstBI       BstEII      BstPI       BstUI       BstXI       
BstZ17I     Bsu36I      CelII       Cfr10I      ClaI        
CpoI        DraI        DraII       DrdI        DsaI        
EagI        Eam1105I    EclXI       Eco52I      Eco81I      
EcoNI       EcoO109I    EcoO65I     EcoRI       EcoRV       
EcoT14I     EcoT22I     FbaI        FseI        HindIII     
HpaI        KasI        KpnI        KspI        MamI        
MluI        MroI        MslI        MvnI        NaeI        
NarI        NciI        NcoI        NdeI        NgoMIV      
NheI        NotI        NruI        NsiI        NspV        
PacI        PaeR7I      PflFI       PinAI       PmaCI       
PmeI        PmlI        PpuMI       PshAI       PshBI       
Psp1406I    PvuI        RsrII       SacI        SacII       
SalI        SapI        SbfI        ScaI        SexAI       
SfiI        SfoI        SfuI        SgrAI       SmaI        
SmlI        SnaBI       SpeI        SphI        Sse8387I    
SspBI       SspI        StyI        SwaI        TfiI        
Tth111I     XbaI        XcmI        XhoI        XmaCI       
XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   39 enzymes) #
###################################

AatII         462  GACGTC               GACGT^C
AcyI          462  GACGTC               G[GA]^CG[CT]C
Aor51HI       689  AGCGCT               AGC^GCT
AvaII         165  GGACC                G^G[AT]CC
AviII         930  TGCGCA               TGC^GCA
BanI           90  GGCACC               G^G[CT][GA]CC
BbsI          459  GAAGAC               GAAGAC(2/6)
BbvCI         306  CCTCAGC              CCTCAGC(-5/-2)
BglII          42  AGATCT               A^GATCT
BpuAI         459  GAAGAC               GAAGAC(2/6)
BsaAI         823  TACGTG               [CT]AC^GT[GA]
BsaHI         462  GACGTC               G[GA]^CG[CT]C
BsiEI         182  CGGTCG               CG[GA][CT]^CG
BspHI         920  TCATGA               T^CATGA
BspMI         410  ACCTGC               ACCTGC(4/8)
BssSI         751  CACGAG               CACGAG(-5/-1)
BstAPI        116  GCAACGCCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
DraIII        129  CACCCAGTG            CAC[ACGT][ACGT][ACGT]^GTG
EarI          943  GAAGAG               CTCTTC(1/4)
Eco47III      689  AGCGCT               AGC^GCT
FauI          635  CCCGC                CCCGC(4/6)
FspI          930  TGCGCA               TGC^GCA
HaeII         689  AGCGCT               [GA]GCGC^[CT]
HapII         282  CCGG                 C^CGG
HgaI          806  GACGC                GACGC(5/10)
Hin1I         462  GACGTC               G[GA]^CG[CT]C
HpaII         282  CCGG                 C^CGG
Ksp632I       943  GAAGAG               CTCTTC(1/4)
MfeI          578  CAATTG               C^AATTG
MseI          404  TTAA                 T^TAA
MspI          282  CCGG                 C^CGG
MunI          578  CAATTG               C^AATTG
PstI          235  CTGCAG               CTGCA^G
RcaI          920  TCATGA               T^CATGA
StuI          912  AGGCCT               AGG^CCT
TaqI          655  TCGA                 T^CGA
Tru9I         404  TTAA                 T^TAA
Tsp509I       579  AATT                 ^AATT
TthHB8I       655  TCGA                 T^CGA


###################################
# TWO CUT ENZYMES (   23 enzymes) #
###################################

AflIII        154  ACATGT               A^C[GA][CT]GT
              221  ACATGT              
AlwI          594  GATCC                GGATC(4/5)
              209  GGATC               
AspHI         319  GAGCAC               G[AT]GC[AT]^C
              670  GTGCTC              
BalI          776  TGGCCA               TGG^CCA
              827  TGGCCA              
BmyI          319  GAGCAC               G[AGT]GC[ACT]^C
              670  GTGCTC              
BseRI          76  GAGGAG               GAGGAG(10/8)
              571  GAGGAG              
BsiHKAI       319  GAGCAC               G[AT]GC[AT]^C
              670  GTGCTC              
Bsp1286I      319  GAGCAC               G[AGT]GC[ACT]^C
              670  GTGCTC              
BspLU11I      154  ACATGT               A^CATGT
              221  ACATGT              
BstYI          42  AGATCT               [GA]^GATC[CT]
              593  AGATCC              
Cfr13I        165  GGACC                G^G[ACGT]CC
              764  GGCCC               
EaeI          776  TGGCCA               [CT]^GGCC[GA]
              827  TGGCCA              
HinfI          68  GAGTC                G^A[ACGT]TC
              657  GACTC               
MaeII         463  ACGT                 A^CGT
              824  ACGT                
MflI           42  AGATCT               [GA]^GATC[CT]
              593  AGATCC              
MluNI         776  TGGCCA               TGG^CCA
              827  TGGCCA              
MscI          776  TGGCCA               TGG^CCA
              827  TGGCCA              
PflMI         768  CCAGGGTCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              879  CCAGCACATGG         
PleI          657  GACTC                GAGTC(4/5)
               68  GAGTC               
Sau96I        165  GGACC                G^G[ACGT]CC
              764  GGCCC               
SfcI          235  CTGCAG               C^T[GA][CT]AG
              267  CTGTAG              
Van91I        768  CCAGGGTCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              879  CCAGCACATGG         
XhoII          42  AGATCT               [GA]^GATC[CT]
              593  AGATCC              


#########################################
# 3 OR MORE CUT ENZYMES (   49 enzymes) #
#########################################

AciI        AfaI        AluI        AlwNI       BbvI        
BfaI        BpmI        BsaJI       BsiYI       BslI        
BsmAI       BsrI        BssKI       BstF5I      BstNI       
Cac8I       CfoI        DdeI        DpnII       EcoRII      
Fnu4HI      FokI        HaeIII      HhaI        HinP1I      
HincII      HindII      HphI        ItaI        MaeI        
MaeIII      MboI        MboII       MnlI        MspA1I      
MvaI        MwoI        NdeII       NlaIII      NlaIV       
NspI        PvuII       RsaI        Sau3AI      ScrFI       
SfaNI       TseI        Tsp45I      TspRI       
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