##################################
# NO CUT ENZYMES ( 147 enzymes) #
##################################
Acc65I AccI AccII AccIII AclI
AcsI AflII AgeI AhdI Alw44I
AocI ApaI ApaLI ApoI AscI
AseI AsnI Asp700I Asp718I AspEI
AspI AvaI AvrII BamHI BanII
BbeI BbrPI BcgI BciVI BclI
BcnI BfrI BglI BlnI BlpI
Bpu1102I BsaBI BsaI BsaWI BseAI
BsgI BsiWI BsmBI BsmFI BsmI
BsoBI Bsp1407I BspDI BspEI BsrBI
BsrDI BsrFI BsrGI BssHII Bst1107I
BstBI BstEII BstPI BstUI BstXI
BstZ17I Bsu36I CelII Cfr10I ClaI
CpoI DraI DraII DrdI DsaI
EagI Eam1105I EclXI Eco52I Eco81I
EcoNI EcoO109I EcoO65I EcoRI EcoRV
EcoT14I EcoT22I FbaI FseI HindIII
HpaI KasI KpnI KspI MamI
MluI MroI MslI MvnI NaeI
NarI NciI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PvuI RsrII SacI SacII
SalI SapI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SmaI
SmlI SnaBI SpeI SphI Sse8387I
SspBI SspI StyI SwaI TfiI
Tth111I XbaI XcmI XhoI XmaCI
XmaI XmnI
###################################
# ONE CUT ENZYMES ( 39 enzymes) #
###################################
AatII 462 GACGTC GACGT^C
AcyI 462 GACGTC G[GA]^CG[CT]C
Aor51HI 689 AGCGCT AGC^GCT
AvaII 165 GGACC G^G[AT]CC
AviII 930 TGCGCA TGC^GCA
BanI 90 GGCACC G^G[CT][GA]CC
BbsI 459 GAAGAC GAAGAC(2/6)
BbvCI 306 CCTCAGC CCTCAGC(-5/-2)
BglII 42 AGATCT A^GATCT
BpuAI 459 GAAGAC GAAGAC(2/6)
BsaAI 823 TACGTG [CT]AC^GT[GA]
BsaHI 462 GACGTC G[GA]^CG[CT]C
BsiEI 182 CGGTCG CG[GA][CT]^CG
BspHI 920 TCATGA T^CATGA
BspMI 410 ACCTGC ACCTGC(4/8)
BssSI 751 CACGAG CACGAG(-5/-1)
BstAPI 116 GCAACGCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
DraIII 129 CACCCAGTG CAC[ACGT][ACGT][ACGT]^GTG
EarI 943 GAAGAG CTCTTC(1/4)
Eco47III 689 AGCGCT AGC^GCT
FauI 635 CCCGC CCCGC(4/6)
FspI 930 TGCGCA TGC^GCA
HaeII 689 AGCGCT [GA]GCGC^[CT]
HapII 282 CCGG C^CGG
HgaI 806 GACGC GACGC(5/10)
Hin1I 462 GACGTC G[GA]^CG[CT]C
HpaII 282 CCGG C^CGG
Ksp632I 943 GAAGAG CTCTTC(1/4)
MfeI 578 CAATTG C^AATTG
MseI 404 TTAA T^TAA
MspI 282 CCGG C^CGG
MunI 578 CAATTG C^AATTG
PstI 235 CTGCAG CTGCA^G
RcaI 920 TCATGA T^CATGA
StuI 912 AGGCCT AGG^CCT
TaqI 655 TCGA T^CGA
Tru9I 404 TTAA T^TAA
Tsp509I 579 AATT ^AATT
TthHB8I 655 TCGA T^CGA
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AflIII 154 ACATGT A^C[GA][CT]GT
221 ACATGT
AlwI 594 GATCC GGATC(4/5)
209 GGATC
AspHI 319 GAGCAC G[AT]GC[AT]^C
670 GTGCTC
BalI 776 TGGCCA TGG^CCA
827 TGGCCA
BmyI 319 GAGCAC G[AGT]GC[ACT]^C
670 GTGCTC
BseRI 76 GAGGAG GAGGAG(10/8)
571 GAGGAG
BsiHKAI 319 GAGCAC G[AT]GC[AT]^C
670 GTGCTC
Bsp1286I 319 GAGCAC G[AGT]GC[ACT]^C
670 GTGCTC
BspLU11I 154 ACATGT A^CATGT
221 ACATGT
BstYI 42 AGATCT [GA]^GATC[CT]
593 AGATCC
Cfr13I 165 GGACC G^G[ACGT]CC
764 GGCCC
EaeI 776 TGGCCA [CT]^GGCC[GA]
827 TGGCCA
HinfI 68 GAGTC G^A[ACGT]TC
657 GACTC
MaeII 463 ACGT A^CGT
824 ACGT
MflI 42 AGATCT [GA]^GATC[CT]
593 AGATCC
MluNI 776 TGGCCA TGG^CCA
827 TGGCCA
MscI 776 TGGCCA TGG^CCA
827 TGGCCA
PflMI 768 CCAGGGTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
879 CCAGCACATGG
PleI 657 GACTC GAGTC(4/5)
68 GAGTC
Sau96I 165 GGACC G^G[ACGT]CC
764 GGCCC
SfcI 235 CTGCAG C^T[GA][CT]AG
267 CTGTAG
Van91I 768 CCAGGGTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
879 CCAGCACATGG
XhoII 42 AGATCT [GA]^GATC[CT]
593 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 49 enzymes) #
#########################################
AciI AfaI AluI AlwNI BbvI
BfaI BpmI BsaJI BsiYI BslI
BsmAI BsrI BssKI BstF5I BstNI
Cac8I CfoI DdeI DpnII EcoRII
Fnu4HI FokI HaeIII HhaI HinP1I
HincII HindII HphI ItaI MaeI
MaeIII MboI MboII MnlI MspA1I
MvaI MwoI NdeII NlaIII NlaIV
NspI PvuII RsaI Sau3AI ScrFI
SfaNI TseI Tsp45I TspRI