Restriction map for
pF1KB0917
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# NO CUT ENZYMES ( 185 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcsI AcyI AfaI AflII
AflIII AgeI AhdI Alw44I AlwI
AocI Aor51HI ApaI ApaLI ApoI
AscI AseI AsnI Asp700I Asp718I
AspEI AspHI AspI AvaI AviII
AvrII BalI BamHI BanII BbeI
BbrPI BbsI BbvCI BciVI BclI
BcnI BfrI BglI BglII BlnI
BlpI BmyI Bpu1102I BpuAI BsaAI
BsaHI BsaWI BseAI BsiEI BsiHKAI
BsiWI BsmFI BsmI BsoBI Bsp1286I
Bsp1407I BspDI BspEI BspHI BspLU11I
BsrBI BsrFI BsrGI BssHII BssSI
Bst1107I BstAPI BstBI BstF5I BstUI
BstXI BstYI BstZ17I Bsu36I CelII
CfoI Cfr10I ClaI CpoI DdeI
DpnII DraI DraIII DsaI EaeI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoNI EcoRI EcoRV FbaI
FokI FseI FspI HaeII HapII
HgaI HhaI Hin1I HinP1I HincII
HindII HindIII HpaI HpaII HphI
KasI KpnI KspI MaeII MboI
MflI MluI MluNI MroI MscI
MspI MvnI NaeI NarI NciI
NcoI NdeI NdeII NgoMIV NheI
NlaIII NotI NruI NspI NspV
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PstI PvuI RcaI RsaI
RsrII SacI SacII SalI Sau3AI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmaI SmlI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI SwaI Tth111I Van91I XbaI
XhoI XhoII XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 46 enzymes) #
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AlwNI 263 CAGTCACTG CAG[ACGT][ACGT][ACGT]^CTG
AvaII 24 GGACC G^G[AT]CC
BanI 15 GGTGCC G^G[CT][GA]CC
BbvI 222 GCAGC GCAGC(8/12)
BcgI 20 CGAAGGACCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI 158 CTAG C^TAG
BpmI 7 CTGGAG CTGGAG(16/14)
BsaBI 109 GATGCATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 210 GGTCTC GGTCTC(1/5)
BseRI 311 GAGGAG GAGGAG(10/8)
BsgI 153 CTGCAC GTGCAG(16/14)
BsmBI 10 GAGACG CGTCTC(1/5)
BspMI 26 ACCTGC ACCTGC(4/8)
BsrDI 180 CATTGC GCAATG(2/0)
BstEII 130 GGTAACC G^GT[ACGT]ACC
BstPI 130 GGTAACC G^GT[ACGT]ACC
DraII 23 AGGACCT [GA]G^G[ACGT]CC[CT]
DrdI 202 GACAGCTGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 162 CTCTTC CTCTTC(1/4)
EcoO109I 23 AGGACCT [GA]G^G[ACGT]CC[CT]
EcoO65I 130 GGTAACC G^GT[ACGT]ACC
EcoT14I 282 CCAAGG C^C[AT][AT]GG
EcoT22I 110 ATGCAT ATGCA^T
Ksp632I 162 CTCTTC CTCTTC(1/4)
MaeI 158 CTAG C^TAG
MamI 109 GATGCATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MboII 163 TCTTC GAAGA(8/7)
MfeI 102 CAATTG C^AATTG
MseI 239 TTAA T^TAA
MslI 180 CATTGCGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 204 CAGCTG C[AC]G^C[GT]G
MunI 102 CAATTG C^AATTG
NlaIV 15 GGTGCC GG[ACGT]^[ACGT]CC
NsiI 110 ATGCAT ATGCA^T
PpuMI 23 AGGACCT [GA]G^G[AT]CC[CT]
PvuII 204 CAGCTG CAG^CTG
SapI 161 GCTCTTC GCTCTTC(1/4)
SfcI 71 CTATAG C^T[GA][CT]AG
StyI 282 CCAAGG C^C[AT][AT]GG
TaqI 309 TCGA T^CGA
Tru9I 239 TTAA T^TAA
TseI 222 GCAGC G^C[AT]GC
Tsp509I 103 AATT ^AATT
TspRI 267 CACTG CA[GC]TG[ACGT][ACGT]^
TthHB8I 309 TCGA T^CGA
XcmI 196 CCAGAGGACAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 19 enzymes) #
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BsaJI 5 CCCTGG C^C[ACGT][ACGT]GG
282 CCAAGG
BsiYI 6 CCTGGAGACGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
135 CCGAGAGATGG
BslI 6 CCTGGAGACGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
135 CCGAGAGATGG
BsrI 268 ACTGG ACTGG(1/-1)
78 CCAGT
BssKI 6 CCTGG ^CC[ACGT]GG
244 CCAGG
BstNI 6 CCTGG CC^[AT]GG
244 CCAGG
Cac8I 39 GCGGGC GC[ACGT]^[ACGT]GC
275 GCCTGC
EcoRII 6 CCTGG ^CC[AT]GG
244 CCAGG
FauI 39 GCGGG CCCGC(4/6)
294 GCGGG
Fnu4HI 30 GCGGC GC^[ACGT]GC
222 GCAGC
ItaI 30 GCGGC GC^[ACGT]GC
222 GCAGC
MnlI 199 GAGG CCTC(7/6)
311 GAGG
MvaI 6 CCTGG CC^[AT]GG
244 CCAGG
MwoI 30 GCGGCATCTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
51 GCTGTGTTCGC
PleI 192 GAGTC GAGTC(4/5)
227 GAGTC
ScrFI 6 CCTGG CC^[ACGT]GG
244 CCAGG
SfaNI 109 GATGC GCATC(5/9)
33 GCATC
TfiI 176 GAATC G^A[AT]TC
287 GAATC
Tsp45I 98 GTGAC ^GT[GC]AC
265 GTCAC
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# 3 OR MORE CUT ENZYMES ( 8 enzymes) #
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AciI AluI BsmAI Cfr13I HaeIII
HinfI MaeIII Sau96I