Restriction map for
pF1KB0918
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# NO CUT ENZYMES ( 142 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AhdI AlwNI AocI Aor51HI ApaI
AscI AseI AsnI Asp700I Asp718I
AspEI AspI AvaII AviII AvrII
BalI BanII BbrPI BbvCI BcgI
BciVI BclI BfaI BlnI BlpI
BpmI Bpu1102I BsaAI BsaI BseAI
BsgI BsiEI BsiWI BsmBI BsmFI
BsmI BspDI BspEI BspMI BsrBI
BsrDI BsrI BssHII BssSI Bst1107I
BstAPI BstEII BstPI BstXI BstZ17I
Bsu36I CelII ClaI CpoI DraII
DraIII DrdI EaeI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO109I EcoO65I EcoRI EcoRV EcoT14I
FbaI FseI FspI HincII HindII
HindIII HpaI HphI KpnI KspI
MaeI MaeII MfeI MluI MluNI
MroI MscI MspA1I MunI NaeI
NcoI NdeI NgoMIV NheI NotI
NruI PacI PaeR7I PflFI PflMI
PleI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PstI PvuI
PvuII RsrII SacI SacII SalI
SbfI ScaI SexAI SfiI SmaI
SnaBI SpeI SphI Sse8387I StuI
StyI SwaI Tsp45I TspRI Tth111I
Van91I XbaI XcmI XhoI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 55 enzymes) #
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AccII 66 CGCG CG^CG
AfaI 565 GTAC GT^AC
AflII 661 CTTAAG C^TTAAG
AflIII 130 ACATGT A^C[GA][CT]GT
AgeI 91 ACCGGT A^CCGGT
AvaI 25 CCCGAG C^[CT]CG[GA]G
BamHI 252 GGATCC G^GATCC
BanI 40 GGCGCC G^G[CT][GA]CC
BbeI 40 GGCGCC GGCGC^C
BbsI 814 GAAGAC GAAGAC(2/6)
BfrI 661 CTTAAG C^TTAAG
BglI 15 GCCGCCCCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 951 AGATCT A^GATCT
BpuAI 814 GAAGAC GAAGAC(2/6)
BsaBI 247 GATCAGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 91 ACCGGT [AT]^CCGG[AT]
BsiYI 547 CCCCTCTCAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 547 CCCCTCTCAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmAI 751 GAGAC GTCTC(1/5)
BsoBI 25 CCCGAG C^[CT]CG[GA]G
Bsp1407I 564 TGTACA T^GTACA
BspHI 201 TCATGA T^CATGA
BspLU11I 130 ACATGT A^CATGT
BsrFI 91 ACCGGT [GA]^CCGG[CT]
BsrGI 564 TGTACA T^GTACA
BstBI 811 TTCGAA TT^CGAA
BstNI 6 CCAGG CC^[AT]GG
BstUI 66 CGCG CG^CG
Cfr10I 91 ACCGGT [GA]^CCGG[CT]
DdeI 552 CTCAG C^T[ACGT]AG
DraI 369 TTTAAA TTT^AAA
DsaI 356 CCGTGG C^C[GA][CT]GG
EcoRII 6 CCAGG ^CC[AT]GG
EcoT22I 860 ATGCAT ATGCA^T
FauI 64 CCCGC CCCGC(4/6)
KasI 40 GGCGCC G^GCGCC
MaeIII 380 GTAAC ^GT[ACGT]AC
MamI 247 GATCAGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MslI 631 CACACAGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvaI 6 CCAGG CC^[AT]GG
MvnI 66 CGCG CG^CG
NarI 40 GGCGCC GG^CGCC
NsiI 860 ATGCAT ATGCA^T
NspV 811 TTCGAA TT^CGAA
PinAI 91 ACCGGT A^CCGGT
RcaI 201 TCATGA T^CATGA
RsaI 565 GTAC GT^AC
SapI 616 GAAGAGC GCTCTTC(1/4)
SfcI 801 CTGTAG C^T[GA][CT]AG
SfoI 40 GGCGCC GGC^GCC
SfuI 811 TTCGAA TT^CGAA
SgrAI 90 CACCGGTG C[GA]^CCGG[CT]G
SmlI 661 CTTAAG C^T[CT][GA]AG
SspBI 564 TGTACA T^GTACA
SspI 708 AATATT AAT^ATT
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AciI 16 CCGC CCGC(-3/-1)
65 CCGC
AcyI 9 GGCGTC G[GA]^CG[CT]C
40 GGCGCC
AluI 195 AGCT AG^CT
902 AGCT
Alw44I 134 GTGCAC G^TGCAC
788 GTGCAC
ApaLI 134 GTGCAC G^TGCAC
788 GTGCAC
BsaHI 9 GGCGTC G[GA]^CG[CT]C
40 GGCGCC
BseRI 79 CTCCTC GAGGAG(10/8)
347 GAGGAG
BstYI 252 GGATCC [GA]^GATC[CT]
951 AGATCT
EarI 616 GAAGAG CTCTTC(1/4)
892 GAAGAG
HaeII 40 GGCGCC [GA]GCGC^[CT]
75 GGCGCT
HgaI 418 GACGC GACGC(5/10)
10 GCGTC
Hin1I 9 GGCGTC G[GA]^CG[CT]C
40 GGCGCC
HinfI 286 GAATC G^A[ACGT]TC
763 GAATC
Ksp632I 616 GAAGAG CTCTTC(1/4)
892 GAAGAG
MflI 252 GGATCC [GA]^GATC[CT]
951 AGATCT
NspI 130 ACATGT [GA]CATG^[CT]
474 ACATGC
SfaNI 637 GATGC GCATC(5/9)
862 GCATC
TfiI 286 GAATC G^A[AT]TC
763 GAATC
XhoII 252 GGATCC [GA]^GATC[CT]
951 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 42 enzymes) #
#########################################
AcsI AlwI ApoI AspHI BbvI
BcnI BmyI BsaJI BsiHKAI Bsp1286I
BssKI BstF5I Cac8I CfoI Cfr13I
DpnII Fnu4HI FokI HaeIII HapII
HhaI HinP1I HpaII ItaI MboI
MboII MnlI MseI MspI MwoI
NciI NdeII NlaIII NlaIV Sau3AI
Sau96I ScrFI TaqI Tru9I TseI
Tsp509I TthHB8I