Restriction map for
pF1KB0926
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# NO CUT ENZYMES ( 138 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcsI AflII AflIII AgeI AhdI
Alw44I AlwI Aor51HI ApaLI ApoI
AscI AseI AsnI Asp700I Asp718I
AspEI AvrII BalI BamHI BbeI
BcgI BciVI BclI BfrI BglII
BlnI BlpI Bpu1102I BsaBI BsaI
BsaWI BseAI BsiEI BsiWI BsmBI
BsmI Bsp1407I BspDI BspEI BspLU11I
BsrDI BsrFI BsrGI BsrI BssHII
Bst1107I BstBI BstEII BstPI BstXI
BstYI BstZ17I CelII Cfr10I ClaI
CpoI DpnII DraI DraIII DrdI
EagI Eam1105I EclXI Eco47III Eco52I
EcoO65I EcoRI EcoRV EcoT22I FbaI
FseI HaeII HincII HindII HindIII
HpaI KasI KpnI MamI MboI
MfeI MflI MluI MluNI MroI
MscI MunI NaeI NarI NcoI
NdeI NdeII NgoMIV NheI NotI
NruI NsiI NspI NspV PacI
PaeR7I PflMI PinAI PmeI PshAI
PshBI Psp1406I PvuI PvuII RsrII
SalI Sau3AI SbfI ScaI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspBI SspI SwaI
Tsp509I Van91I XbaI XhoI XhoII
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 57 enzymes) #
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AccII 26 CGCG CG^CG
AcyI 895 GACGCC G[GA]^CG[CT]C
AocI 61 CCTCAGG CC^T[ACGT]AGG
AspHI 928 GAGCTC G[AT]GC[AT]^C
AspI 982 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 196 CCCGAG C^[CT]CG[GA]G
AviII 819 TGCGCA TGC^GCA
BanI 729 GGCACC G^G[CT][GA]CC
BbrPI 922 CACGTG CAC^GTG
BcnI 180 CCCGG CC^[GC]GG
BfaI 701 CTAG C^TAG
BsaAI 922 CACGTG [CT]AC^GT[GA]
BsaHI 895 GACGCC G[GA]^CG[CT]C
BsgI 169 GTGCAG GTGCAG(16/14)
BsiHKAI 928 GAGCTC G[AT]GC[AT]^C
BsoBI 196 CCCGAG C^[CT]CG[GA]G
BspHI 989 TCATGA T^CATGA
BspMI 341 ACCTGC ACCTGC(4/8)
BsrBI 199 GAGCGG CCGCTC(-3/-3)
BssSI 943 CACGAG CACGAG(-5/-1)
BstAPI 239 GCATCGACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 26 CGCG CG^CG
Bsu36I 61 CCTCAGG CC^T[ACGT]AGG
Eco81I 61 CCTCAGG CC^T[ACGT]AGG
EcoNI 443 CCTTCCCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT14I 660 CCTTGG C^C[AT][AT]GG
FspI 819 TGCGCA TGC^GCA
HapII 181 CCGG C^CGG
Hin1I 895 GACGCC G[GA]^CG[CT]C
HpaII 181 CCGG C^CGG
KspI 25 CCGCGG CCGC^GG
MaeI 701 CTAG C^TAG
MseI 590 TTAA T^TAA
MslI 984 CATGGTCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI 181 CCGG C^CGG
MvnI 26 CGCG CG^CG
NciI 180 CCCGG CC^[GC]GG
PflFI 982 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PleI 3 GACTC GAGTC(4/5)
PmaCI 922 CACGTG CAC^GTG
PmlI 922 CACGTG CAC^GTG
PpuMI 1014 GGGACCC [GA]G^G[AT]CC[CT]
RcaI 989 TCATGA T^CATGA
SacI 928 GAGCTC GAGCT^C
SacII 25 CCGCGG CCGC^GG
SapI 930 GCTCTTC GCTCTTC(1/4)
SexAI 533 ACCTGGT A^CC[AT]GGT
SfiI 194 GGCCCGAGCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmlI 801 CTCAAG C^T[CT][GA]AG
StuI 742 AGGCCT AGG^CCT
StyI 660 CCTTGG C^C[AT][AT]GG
TaqI 242 TCGA T^CGA
TfiI 502 GAATC G^A[AT]TC
Tru9I 590 TTAA T^TAA
Tth111I 982 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 242 TCGA T^CGA
XcmI 657 CCACCTTGGGGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 19 enzymes) #
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ApaI 145 GGGCCC GGGCC^C
193 GGGCCC
BbsI 268 GTCTTC GAAGAC(2/6)
711 GTCTTC
BbvCI 48 CCTCAGC CCTCAGC(-5/-2)
738 GCTGAGG
BglI 94 GCCCCACAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
195 GCCCGAGCGGC
BpuAI 268 GTCTTC GAAGAC(2/6)
711 GTCTTC
BseRI 615 CTCCTC GAGGAG(10/8)
618 CTCCTC
BsmAI 650 GAGAC GTCTC(1/5)
313 GTCTC
CfoI 248 GCGC GCG^C
820 GCGC
EaeI 202 CGGCCA [CT]^GGCC[GA]
835 CGGCCA
HgaI 67 GACGC GACGC(5/10)
895 GACGC
HhaI 248 GCGC GCG^C
820 GCGC
HinP1I 248 GCGC G^CGC
820 GCGC
HinfI 3 GACTC G^A[ACGT]TC
502 GAATC
MaeII 875 ACGT A^CGT
923 ACGT
MaeIII 517 GTCAC ^GT[ACGT]AC
980 GTGAC
MspA1I 25 CCGCGG C[AC]G^C[GT]G
683 CCGCTG
PstI 217 CTGCAG CTGCA^G
456 CTGCAG
Tsp45I 517 GTCAC ^GT[GC]AC
980 GTGAC
TspRI 459 CAGTG CA[GC]TG[ACGT][ACGT]^
1022 CACTG
#########################################
# 3 OR MORE CUT ENZYMES ( 44 enzymes) #
#########################################
AciI AfaI AluI AlwNI AvaII
BanII BbvI BmyI BpmI BsaJI
BsiYI BslI BsmFI Bsp1286I BssKI
BstF5I BstNI Cac8I Cfr13I DdeI
DraII DsaI EarI EcoO109I EcoRII
FauI Fnu4HI FokI HaeIII HphI
ItaI Ksp632I MboII MnlI MvaI
MwoI NlaIII NlaIV RsaI Sau96I
ScrFI SfaNI SfcI TseI