Restriction map for
pF1KB0930
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# NO CUT ENZYMES ( 130 enzymes) #
##################################
AatII Acc65I AccI AclI AcsI
AfaI AflII AflIII AgeI AhdI
AlwI AlwNI AocI Aor51HI ApoI
AscI AseI AsnI Asp718I AspEI
AviII AvrII BamHI BanI BbeI
BcgI BciVI BfaI BfrI BglI
BglII BlnI BsaBI BsiWI BsmI
Bsp1407I BspDI BspHI BspLU11I BsrDI
BsrGI Bst1107I BstAPI BstBI BstEII
BstPI BstXI BstYI BstZ17I Bsu36I
ClaI CpoI DraI DraIII DrdI
Eam1105I Eco47III Eco81I EcoNI EcoO65I
EcoRI EcoRV EcoT22I FseI FspI
HincII HindII HindIII HpaI KasI
KpnI KspI MaeI MamI MfeI
MflI MluI MseI MslI MunI
NarI NcoI NdeI NheI NotI
NruI NsiI NspI NspV PacI
PaeR7I PflMI PinAI PmeI PshAI
PshBI Psp1406I PvuI RcaI RsaI
RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspBI SspI SwaI
TfiI Tru9I Tsp509I Van91I XbaI
XcmI XhoI XhoII XmaCI XmaI
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# ONE CUT ENZYMES ( 47 enzymes) #
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AccIII 890 TCCGGA T^CCGGA
AcyI 424 GACGCC G[GA]^CG[CT]C
ApaI 89 GGGCCC GGGCC^C
AspI 163 GACCTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 619 CCCGAG C^[CT]CG[GA]G
BalI 38 TGGCCA TGG^CCA
BbsI 205 GAAGAC GAAGAC(2/6)
BbvCI 906 CCTCAGC CCTCAGC(-5/-2)
BclI 291 TGATCA T^GATCA
BpmI 410 CTCCAG CTGGAG(16/14)
BpuAI 205 GAAGAC GAAGAC(2/6)
BsaHI 424 GACGCC G[GA]^CG[CT]C
BsaI 216 GAGACC GGTCTC(1/5)
BseAI 890 TCCGGA T^CCGGA
BsmBI 168 CGTCTC CGTCTC(1/5)
BsoBI 619 CCCGAG C^[CT]CG[GA]G
BspEI 890 TCCGGA T^CCGGA
BspMI 653 ACCTGC ACCTGC(4/8)
BsrBI 853 GAGCGG CCGCTC(-3/-3)
BsrFI 566 GCCGGC [GA]^CCGG[CT]
BsrI 19 CCAGT ACTGG(1/-1)
BssHII 507 GCGCGC G^CGCGC
BssSI 780 CTCGTG CACGAG(-5/-1)
Cfr10I 566 GCCGGC [GA]^CCGG[CT]
DpnII 292 GATC ^GATC
EarI 1020 GAAGAG CTCTTC(1/4)
FbaI 291 TGATCA T^GATCA
HgaI 424 GACGC GACGC(5/10)
Hin1I 424 GACGCC G[GA]^CG[CT]C
HinfI 1092 GACTC G^A[ACGT]TC
Ksp632I 1020 GAAGAG CTCTTC(1/4)
MboI 292 GATC ^GATC
MluNI 38 TGGCCA TGG^CCA
MroI 890 TCCGGA T^CCGGA
MscI 38 TGGCCA TGG^CCA
NaeI 566 GCCGGC GCC^GGC
NdeII 292 GATC ^GATC
NgoMIV 566 GCCGGC G^CCGGC
NlaIII 6 CATG CATG^
PflFI 163 GACCTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PleI 1092 GACTC GAGTC(4/5)
PpuMI 243 GGGACCC [GA]G^G[AT]CC[CT]
Sau3AI 292 GATC ^GATC
SmlI 51 CTCAAG C^T[CT][GA]AG
TaqI 611 TCGA T^CGA
Tth111I 163 GACCTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 611 TCGA T^CGA
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# TWO CUT ENZYMES ( 24 enzymes) #
###################################
Alw44I 589 GTGCAC G^TGCAC
841 GTGCAC
ApaLI 589 GTGCAC G^TGCAC
841 GTGCAC
BanII 89 GGGCCC G[GA]GC[CT]^C
305 GGGCTC
BbrPI 322 CACGTG CAC^GTG
537 CACGTG
BlpI 552 GCTCAGC GC^T[ACGT]AGC
742 GCTCAGC
Bpu1102I 552 GCTCAGC GC^T[ACGT]AGC
742 GCTCAGC
BsaAI 322 CACGTG [CT]AC^GT[GA]
537 CACGTG
BseRI 58 GAGGAG GAGGAG(10/8)
190 GAGGAG
BsgI 1009 CTGCAC GTGCAG(16/14)
235 GTGCAG
BsiEI 478 CGGCCG CG[GA][CT]^CG
856 CGGCCG
CelII 552 GCTCAGC GC^T[ACGT]AGC
742 GCTCAGC
DsaI 196 CCGTGG C^C[GA][CT]GG
434 CCACGG
EagI 478 CGGCCG C^GGCCG
856 CGGCCG
EclXI 478 CGGCCG C^GGCCG
856 CGGCCG
Eco52I 478 CGGCCG C^GGCCG
856 CGGCCG
HphI 579 TCACC GGTGA(8/7)
1062 TCACC
MaeIII 22 GTGAC ^GT[ACGT]AC
1061 GTCAC
PmaCI 322 CACGTG CAC^GTG
537 CACGTG
PmlI 322 CACGTG CAC^GTG
537 CACGTG
PstI 269 CTGCAG CTGCA^G
655 CTGCAG
PvuII 555 CAGCTG CAG^CTG
835 CAGCTG
SfcI 269 CTGCAG C^T[GA][CT]AG
655 CTGCAG
StuI 605 AGGCCT AGG^CCT
704 AGGCCT
Tsp45I 22 GTGAC ^GT[GC]AC
1061 GTCAC
#########################################
# 3 OR MORE CUT ENZYMES ( 57 enzymes) #
#########################################
AccII AciI AluI Asp700I AspHI
AvaII BbvI BcnI BmyI BsaJI
BsaWI BsiHKAI BsiYI BslI BsmAI
BsmFI Bsp1286I BssKI BstF5I BstNI
BstUI Cac8I CfoI Cfr13I DdeI
DraII EaeI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HhaI HinP1I HpaII ItaI
MaeII MboII MnlI MspA1I MspI
MvaI MvnI MwoI NciI NlaIV
Sau96I ScrFI SfaNI StyI TseI
TspRI XmnI