Restriction map for pF1KB0931
##################################
# NO CUT ENZYMES (  176 enzymes) #
##################################

AatII       Acc65I      AccII       AccIII      AclI        
AcsI        AcyI        AfaI        AflII       AflIII      
AgeI        AhdI        AluI        Alw44I      AlwNI       
Aor51HI     ApaLI       ApoI        AscI        AseI        
AsnI        Asp700I     Asp718I     AspEI       AspHI       
AspI        AvaI        AviII       AvrII       BamHI       
BbeI        BbrPI       BbsI        BbvCI       BcgI        
BciVI       BclI        BcnI        BfaI        BfrI        
BglII       BlnI        BlpI        Bpu1102I    BpuAI       
BsaAI       BsaBI       BsaHI       BsaI        BsaWI       
BseAI       BseRI       BsiEI       BsiHKAI     BsiWI       
BsmBI       BsmI        BsoBI       Bsp1407I    BspDI       
BspEI       BspLU11I    BsrBI       BsrDI       BsrGI       
BssHII      BssSI       Bst1107I    BstBI       BstEII      
BstPI       BstUI       BstZ17I     CelII       CfoI        
ClaI        CpoI        DraI        DraIII      DrdI        
EagI        Eam1105I    EclXI       Eco47III    Eco52I      
EcoNI       EcoO65I     EcoRI       EcoRV       EcoT22I     
FauI        FbaI        FseI        FspI        HaeII       
HgaI        HhaI        Hin1I       HinP1I      HincII      
HindII      HindIII     HinfI       HpaI        KasI        
KpnI        KspI        MaeI        MamI        MfeI        
MluI        MroI        MseI        MspA1I      MunI        
MvnI        NaeI        NarI        NciI        NdeI        
NgoMIV      NheI        NotI        NruI        NsiI        
NspI        NspV        PacI        PaeR7I      PflFI       
PflMI       PinAI       PleI        PmaCI       PmeI        
PmlI        PshAI       PshBI       Psp1406I    PstI        
PvuI        PvuII       RsaI        RsrII       SacI        
SacII       SalI        SapI        SbfI        ScaI        
SexAI       SfiI        SfoI        SfuI        SgrAI       
SmaI        SmlI        SnaBI       SpeI        SphI        
Sse8387I    SspBI       SspI        StuI        SwaI        
TfiI        Tru9I       Tsp509I     Tth111I     Van91I      
XbaI        XcmI        XhoI        XmaCI       XmaI        
XmnI        


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# ONE CUT ENZYMES (   40 enzymes) #
###################################

AccI          244  GTAGAC               GT^[AC][GT]AC
AlwI          192  GATCC                GGATC(4/5)
AocI           59  CCTCAGG              CC^T[ACGT]AGG
ApaI           55  GGGCCC               GGGCC^C
BalI          207  TGGCCA               TGG^CCA
BanI          372  GGTGCC               G^G[CT][GA]CC
BanII          55  GGGCCC               G[GA]GC[CT]^C
BglI           57  GCCCTCAGGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BmyI           55  GGGCCC               G[AGT]GC[ACT]^C
BpmI          175  CTCCAG               CTGGAG(16/14)
Bsp1286I       55  GGGCCC               G[AGT]GC[ACT]^C
BspHI         413  TCATGA               T^CATGA
BspMI         224  ACCTGC               ACCTGC(4/8)
BsrFI         422  ACCGGC               [GA]^CCGG[CT]
BsrI          376  CCAGT                ACTGG(1/-1)
BstAPI        262  GCAGGCTATGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI         356  CCATATTTATGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI         191  AGATCC               [GA]^GATC[CT]
Bsu36I         59  CCTCAGG              CC^T[ACGT]AGG
Cac8I         262  GCAGGC               GC[ACGT]^[ACGT]GC
Cfr10I        422  ACCGGC               [GA]^CCGG[CT]
DsaI          392  CCATGG               C^C[GA][CT]GG
EaeI          207  TGGCCA               [CT]^GGCC[GA]
Eco81I         59  CCTCAGG              CC^T[ACGT]AGG
EcoT14I       392  CCATGG               C^C[AT][AT]GG
HapII         423  CCGG                 C^CGG
HpaII         423  CCGG                 C^CGG
MaeII         128  ACGT                 A^CGT
MflI          191  AGATCC               [GA]^GATC[CT]
MluNI         207  TGGCCA               TGG^CCA
MscI          207  TGGCCA               TGG^CCA
MspI          423  CCGG                 C^CGG
NcoI          392  CCATGG               C^CATGG
PpuMI         338  GGGACCC              [GA]G^G[AT]CC[CT]
RcaI          413  TCATGA               T^CATGA
StyI          392  CCATGG               C^C[AT][AT]GG
TaqI          327  TCGA                 T^CGA
Tsp45I         25  GTCAC                ^GT[GC]AC
TthHB8I       327  TCGA                 T^CGA
XhoII         191  AGATCC               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   13 enzymes) #
###################################

AvaII         313  GGACC                G^G[AT]CC
              339  GGACC               
BsiYI         161  CCCTGGATGGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
              387  CCTCACCATGG         
BslI          161  CCCTGGATGGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
              387  CCTCACCATGG         
BsmAI         106  GTCTC                GTCTC(1/5)
              276  GTCTC               
BsmFI          88  GGGAC                GGGAC(10/14)
              338  GGGAC               
BstF5I        165  GGATG                GGATG(2/0)
              237  GGATG               
EarI            8  GAAGAG               CTCTTC(1/4)
               34  GAAGAG              
FokI          165  GGATG                GGATG(9/13)
              237  GGATG               
Ksp632I         8  GAAGAG               CTCTTC(1/4)
               34  GAAGAG              
MaeIII         25  GTCAC                ^GT[ACGT]AC
              297  GTTAC               
MboII           8  GAAGA                GAAGA(8/7)
               34  GAAGA               
NlaIII        393  CATG                 CATG^
              414  CATG                
SfcI          242  CTGTAG               C^T[GA][CT]AG
              317  CTATAG              


#########################################
# 3 OR MORE CUT ENZYMES (   29 enzymes) #
#########################################

AciI        BbvI        BsaJI       BsgI        BssKI       
BstNI       Cfr13I      DdeI        DpnII       DraII       
EcoO109I    EcoRII      Fnu4HI      HaeIII      HphI        
ItaI        MboI        MnlI        MslI        MvaI        
MwoI        NdeII       NlaIV       Sau3AI      Sau96I      
ScrFI       SfaNI       TseI        TspRI       
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