################################## # NO CUT ENZYMES ( 176 enzymes) # ################################## AatII Acc65I AccII AccIII AclI AcsI AcyI AfaI AflII AflIII AgeI AhdI AluI Alw44I AlwNI Aor51HI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AvaI AviII AvrII BamHI BbeI BbrPI BbsI BbvCI BcgI BciVI BclI BcnI BfaI BfrI BglII BlnI BlpI Bpu1102I BpuAI BsaAI BsaBI BsaHI BsaI BsaWI BseAI BseRI BsiEI BsiHKAI BsiWI BsmBI BsmI BsoBI Bsp1407I BspDI BspEI BspLU11I BsrBI BsrDI BsrGI BssHII BssSI Bst1107I BstBI BstEII BstPI BstUI BstZ17I CelII CfoI ClaI CpoI DraI DraIII DrdI EagI Eam1105I EclXI Eco47III Eco52I EcoNI EcoO65I EcoRI EcoRV EcoT22I FauI FbaI FseI FspI HaeII HgaI HhaI Hin1I HinP1I HincII HindII HindIII HinfI HpaI KasI KpnI KspI MaeI MamI MfeI MluI MroI MseI MspA1I MunI MvnI NaeI NarI NciI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PleI PmaCI PmeI PmlI PshAI PshBI Psp1406I PstI PvuI PvuII RsaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI TfiI Tru9I Tsp509I Tth111I Van91I XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 40 enzymes) # ################################### AccI 244 GTAGAC GT^[AC][GT]AC AlwI 192 GATCC GGATC(4/5) AocI 59 CCTCAGG CC^T[ACGT]AGG ApaI 55 GGGCCC GGGCC^C BalI 207 TGGCCA TGG^CCA BanI 372 GGTGCC G^G[CT][GA]CC BanII 55 GGGCCC G[GA]GC[CT]^C BglI 57 GCCCTCAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BmyI 55 GGGCCC G[AGT]GC[ACT]^C BpmI 175 CTCCAG CTGGAG(16/14) Bsp1286I 55 GGGCCC G[AGT]GC[ACT]^C BspHI 413 TCATGA T^CATGA BspMI 224 ACCTGC ACCTGC(4/8) BsrFI 422 ACCGGC [GA]^CCGG[CT] BsrI 376 CCAGT ACTGG(1/-1) BstAPI 262 GCAGGCTATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstXI 356 CCATATTTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstYI 191 AGATCC [GA]^GATC[CT] Bsu36I 59 CCTCAGG CC^T[ACGT]AGG Cac8I 262 GCAGGC GC[ACGT]^[ACGT]GC Cfr10I 422 ACCGGC [GA]^CCGG[CT] DsaI 392 CCATGG C^C[GA][CT]GG EaeI 207 TGGCCA [CT]^GGCC[GA] Eco81I 59 CCTCAGG CC^T[ACGT]AGG EcoT14I 392 CCATGG C^C[AT][AT]GG HapII 423 CCGG C^CGG HpaII 423 CCGG C^CGG MaeII 128 ACGT A^CGT MflI 191 AGATCC [GA]^GATC[CT] MluNI 207 TGGCCA TGG^CCA MscI 207 TGGCCA TGG^CCA MspI 423 CCGG C^CGG NcoI 392 CCATGG C^CATGG PpuMI 338 GGGACCC [GA]G^G[AT]CC[CT] RcaI 413 TCATGA T^CATGA StyI 392 CCATGG C^C[AT][AT]GG TaqI 327 TCGA T^CGA Tsp45I 25 GTCAC ^GT[GC]AC TthHB8I 327 TCGA T^CGA XhoII 191 AGATCC [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 13 enzymes) # ################################### AvaII 313 GGACC G^G[AT]CC 339 GGACC BsiYI 161 CCCTGGATGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 387 CCTCACCATGG BslI 161 CCCTGGATGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 387 CCTCACCATGG BsmAI 106 GTCTC GTCTC(1/5) 276 GTCTC BsmFI 88 GGGAC GGGAC(10/14) 338 GGGAC BstF5I 165 GGATG GGATG(2/0) 237 GGATG EarI 8 GAAGAG CTCTTC(1/4) 34 GAAGAG FokI 165 GGATG GGATG(9/13) 237 GGATG Ksp632I 8 GAAGAG CTCTTC(1/4) 34 GAAGAG MaeIII 25 GTCAC ^GT[ACGT]AC 297 GTTAC MboII 8 GAAGA GAAGA(8/7) 34 GAAGA NlaIII 393 CATG CATG^ 414 CATG SfcI 242 CTGTAG C^T[GA][CT]AG 317 CTATAG ######################################### # 3 OR MORE CUT ENZYMES ( 29 enzymes) # ######################################### AciI BbvI BsaJI BsgI BssKI BstNI Cfr13I DdeI DpnII DraII EcoO109I EcoRII Fnu4HI HaeIII HphI ItaI MboI MnlI MslI MvaI MwoI NdeII NlaIV Sau3AI Sau96I ScrFI SfaNI TseI TspRI