##################################
# NO CUT ENZYMES ( 189 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AciI AclI AcyI AflII AflIII
AgeI AhdI Alw44I AlwI Aor51HI
ApaLI AscI AseI AsnI Asp700I
Asp718I AspEI AspI AviII AvrII
BalI BamHI BanI BbeI BbrPI
BbvCI BbvI BcgI BciVI BclI
BcnI BfrI BglI BglII BlnI
BlpI Bpu1102I BsaAI BsaBI BsaHI
BsaI BsaWI BseAI BseRI BsiEI
BsiWI BsmAI BsmBI BsmI Bsp1407I
BspDI BspEI BspHI BspLU11I BspMI
BsrBI BsrDI BsrFI BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstUI BstXI BstYI BstZ17I
CelII CfoI Cfr10I ClaI CpoI
DpnII DraI DraIII DrdI EaeI
EagI Eam1105I EclXI Eco47III Eco52I
EcoNI EcoO65I EcoRI EcoRV EcoT14I
EcoT22I FauI FbaI Fnu4HI FseI
FspI HaeII HapII HgaI HhaI
Hin1I HinP1I HincII HindII HindIII
HpaI HpaII HphI ItaI KasI
KpnI KspI MaeII MaeIII MamI
MboI MfeI MflI MluI MluNI
MroI MscI MslI MspI MunI
MvnI NaeI NarI NciI NcoI
NdeI NdeII NgoMIV NheI NotI
NruI NsiI NspV PacI PflFI
PflMI PinAI PleI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PstI PvuI RcaI RsrII SacII
SalI SapI Sau3AI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspBI StuI StyI SwaI TseI
Tsp45I Tth111I Van91I XbaI XcmI
XhoII XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 35 enzymes) #
###################################
AfaI 89 GTAC GT^AC
AlwNI 52 CAGAATCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 409 CCTCAGG CC^T[ACGT]AGG
ApaI 415 GGGCCC GGGCC^C
AvaI 260 CTCGAG C^[CT]CG[GA]G
BfaI 244 CTAG C^TAG
BpmI 325 CTGGAG CTGGAG(16/14)
BsgI 233 GTGCAG GTGCAG(16/14)
BsmFI 344 GTCCC GGGAC(10/14)
BsoBI 260 CTCGAG C^[CT]CG[GA]G
BssKI 424 CCTGG ^CC[ACGT]GG
BstNI 424 CCTGG CC^[AT]GG
Bsu36I 409 CCTCAGG CC^T[ACGT]AGG
DsaI 399 CCGTGG C^C[GA][CT]GG
EarI 121 CTCTTC CTCTTC(1/4)
Eco81I 409 CCTCAGG CC^T[ACGT]AGG
EcoRII 424 CCTGG ^CC[AT]GG
Ksp632I 121 CTCTTC CTCTTC(1/4)
MaeI 244 CTAG C^TAG
MspA1I 370 CAGCTG C[AC]G^C[GT]G
MvaI 424 CCTGG CC^[AT]GG
MwoI 384 GCACAGTAAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NspI 356 ACATGC [GA]CATG^[CT]
PaeR7I 260 CTCGAG C^TCGAG
PvuII 370 CAGCTG CAG^CTG
RsaI 89 GTAC GT^AC
ScrFI 424 CCTGG CC^[ACGT]GG
SfaNI 33 GATGC GCATC(5/9)
SfcI 367 CTACAG C^T[GA][CT]AG
SmlI 260 CTCGAG C^T[CT][GA]AG
SspI 40 AATATT AAT^ATT
TaqI 261 TCGA T^CGA
TspRI 236 CAGTG CA[GC]TG[ACGT][ACGT]^
TthHB8I 261 TCGA T^CGA
XhoI 260 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AcsI 166 GAATTT [GA]^AATT[CT]
216 GAATTT
ApoI 166 GAATTT [GA]^AATT[CT]
216 GAATTT
AspHI 3 GAGCTC G[AT]GC[AT]^C
226 GAGCTC
AvaII 396 GGACC G^G[AT]CC
403 GGACC
BbsI 247 GAAGAC GAAGAC(2/6)
103 GTCTTC
BpuAI 247 GAAGAC GAAGAC(2/6)
103 GTCTTC
BsaJI 399 CCGTGG C^C[ACGT][ACGT]GG
424 CCTGGG
BsiHKAI 3 GAGCTC G[AT]GC[AT]^C
226 GAGCTC
BsrI 361 CCAGT ACTGG(1/-1)
378 CCAGT
BstF5I 108 CATCC GGATG(2/0)
32 GGATG
Cac8I 291 GCACGC GC[ACGT]^[ACGT]GC
372 GCTGGC
DraII 414 GGGGCCC [GA]G^G[ACGT]CC[CT]
415 GGGCCCC
EcoO109I 414 GGGGCCC [GA]G^G[ACGT]CC[CT]
415 GGGCCCC
FokI 108 CATCC GGATG(9/13)
32 GGATG
HaeIII 375 GGCC GG^CC
416 GGCC
HinfI 47 GATTC G^A[ACGT]TC
54 GAATC
MseI 193 TTAA T^TAA
317 TTAA
SacI 3 GAGCTC GAGCT^C
226 GAGCTC
TfiI 47 GATTC G^A[AT]TC
54 GAATC
Tru9I 193 TTAA T^TAA
317 TTAA
#########################################
# 3 OR MORE CUT ENZYMES ( 14 enzymes) #
#########################################
AluI BanII BmyI BsiYI BslI
Bsp1286I Cfr13I DdeI MboII MnlI
NlaIII NlaIV Sau96I Tsp509I