################################## # NO CUT ENZYMES ( 189 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AciI AclI AcyI AflII AflIII AgeI AhdI Alw44I AlwI Aor51HI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspI AviII AvrII BalI BamHI BanI BbeI BbrPI BbvCI BbvI BcgI BciVI BclI BcnI BfrI BglI BglII BlnI BlpI Bpu1102I BsaAI BsaBI BsaHI BsaI BsaWI BseAI BseRI BsiEI BsiWI BsmAI BsmBI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstXI BstYI BstZ17I CelII CfoI Cfr10I ClaI CpoI DpnII DraI DraIII DrdI EaeI EagI Eam1105I EclXI Eco47III Eco52I EcoNI EcoO65I EcoRI EcoRV EcoT14I EcoT22I FauI FbaI Fnu4HI FseI FspI HaeII HapII HgaI HhaI Hin1I HinP1I HincII HindII HindIII HpaI HpaII HphI ItaI KasI KpnI KspI MaeII MaeIII MamI MboI MfeI MflI MluI MluNI MroI MscI MslI MspI MunI MvnI NaeI NarI NciI NcoI NdeI NdeII NgoMIV NheI NotI NruI NsiI NspV PacI PflFI PflMI PinAI PleI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI RcaI RsrII SacII SalI SapI Sau3AI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI StuI StyI SwaI TseI Tsp45I Tth111I Van91I XbaI XcmI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 35 enzymes) # ################################### AfaI 89 GTAC GT^AC AlwNI 52 CAGAATCTG CAG[ACGT][ACGT][ACGT]^CTG AocI 409 CCTCAGG CC^T[ACGT]AGG ApaI 415 GGGCCC GGGCC^C AvaI 260 CTCGAG C^[CT]CG[GA]G BfaI 244 CTAG C^TAG BpmI 325 CTGGAG CTGGAG(16/14) BsgI 233 GTGCAG GTGCAG(16/14) BsmFI 344 GTCCC GGGAC(10/14) BsoBI 260 CTCGAG C^[CT]CG[GA]G BssKI 424 CCTGG ^CC[ACGT]GG BstNI 424 CCTGG CC^[AT]GG Bsu36I 409 CCTCAGG CC^T[ACGT]AGG DsaI 399 CCGTGG C^C[GA][CT]GG EarI 121 CTCTTC CTCTTC(1/4) Eco81I 409 CCTCAGG CC^T[ACGT]AGG EcoRII 424 CCTGG ^CC[AT]GG Ksp632I 121 CTCTTC CTCTTC(1/4) MaeI 244 CTAG C^TAG MspA1I 370 CAGCTG C[AC]G^C[GT]G MvaI 424 CCTGG CC^[AT]GG MwoI 384 GCACAGTAAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC NspI 356 ACATGC [GA]CATG^[CT] PaeR7I 260 CTCGAG C^TCGAG PvuII 370 CAGCTG CAG^CTG RsaI 89 GTAC GT^AC ScrFI 424 CCTGG CC^[ACGT]GG SfaNI 33 GATGC GCATC(5/9) SfcI 367 CTACAG C^T[GA][CT]AG SmlI 260 CTCGAG C^T[CT][GA]AG SspI 40 AATATT AAT^ATT TaqI 261 TCGA T^CGA TspRI 236 CAGTG CA[GC]TG[ACGT][ACGT]^ TthHB8I 261 TCGA T^CGA XhoI 260 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 20 enzymes) # ################################### AcsI 166 GAATTT [GA]^AATT[CT] 216 GAATTT ApoI 166 GAATTT [GA]^AATT[CT] 216 GAATTT AspHI 3 GAGCTC G[AT]GC[AT]^C 226 GAGCTC AvaII 396 GGACC G^G[AT]CC 403 GGACC BbsI 247 GAAGAC GAAGAC(2/6) 103 GTCTTC BpuAI 247 GAAGAC GAAGAC(2/6) 103 GTCTTC BsaJI 399 CCGTGG C^C[ACGT][ACGT]GG 424 CCTGGG BsiHKAI 3 GAGCTC G[AT]GC[AT]^C 226 GAGCTC BsrI 361 CCAGT ACTGG(1/-1) 378 CCAGT BstF5I 108 CATCC GGATG(2/0) 32 GGATG Cac8I 291 GCACGC GC[ACGT]^[ACGT]GC 372 GCTGGC DraII 414 GGGGCCC [GA]G^G[ACGT]CC[CT] 415 GGGCCCC EcoO109I 414 GGGGCCC [GA]G^G[ACGT]CC[CT] 415 GGGCCCC FokI 108 CATCC GGATG(9/13) 32 GGATG HaeIII 375 GGCC GG^CC 416 GGCC HinfI 47 GATTC G^A[ACGT]TC 54 GAATC MseI 193 TTAA T^TAA 317 TTAA SacI 3 GAGCTC GAGCT^C 226 GAGCTC TfiI 47 GATTC G^A[AT]TC 54 GAATC Tru9I 193 TTAA T^TAA 317 TTAA ######################################### # 3 OR MORE CUT ENZYMES ( 14 enzymes) # ######################################### AluI BanII BmyI BsiYI BslI Bsp1286I Cfr13I DdeI MboII MnlI NlaIII NlaIV Sau96I Tsp509I