Restriction map for
pF1KB0939
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# NO CUT ENZYMES ( 175 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcsI AcyI AfaI AflII
AflIII AgeI AhdI Alw44I AlwI
AlwNI Aor51HI ApaLI ApoI AscI
AseI AsnI Asp718I AspEI AspI
AviII AvrII BalI BamHI BanI
BbeI BbrPI BbsI BbvCI BcgI
BciVI BclI BfrI BglI BglII
BlnI BlpI Bpu1102I BpuAI BsaAI
BsaBI BsaHI BsaWI BseAI BsgI
BsiEI BsiWI BsmBI BsmI Bsp1407I
BspDI BspEI BspHI BspLU11I BsrDI
BsrFI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstUI
BstXI BstYI BstZ17I CelII CfoI
Cfr10I ClaI CpoI DraI DraIII
DrdI DsaI EaeI EagI Eam1105I
EclXI Eco47III Eco52I EcoO65I EcoRI
EcoRV EcoT22I FbaI FseI FspI
HaeII HgaI HhaI Hin1I HinP1I
HincII HindII HindIII HinfI HpaI
KasI KpnI KspI MaeII MamI
MfeI MflI MluI MluNI MroI
MscI MseI MslI MunI MvnI
NaeI NarI NcoI NdeI NgoMIV
NheI NlaIII NotI NruI NsiI
NspI NspV PacI PflFI PinAI
PleI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI PvuII RcaI
RsaI RsrII SacII SalI SapI
SbfI ScaI SfaNI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
SwaI TfiI Tru9I Tsp509I Tth111I
XbaI XcmI XhoII XmaCI XmaI
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# ONE CUT ENZYMES ( 43 enzymes) #
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AocI 49 CCTCAGG CC^T[ACGT]AGG
ApaI 42 GGGCCC GGGCC^C
Asp700I 187 GAACTCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 400 GAGCTC G[AT]GC[AT]^C
AvaI 301 CTCGAG C^[CT]CG[GA]G
BbvI 414 GCTGC GCAGC(8/12)
BcnI 458 CCCGG CC^[GC]GG
BfaI 277 CTAG C^TAG
BsaI 12 GAGACC GGTCTC(1/5)
BsiHKAI 400 GAGCTC G[AT]GC[AT]^C
BsmAI 12 GAGAC GTCTC(1/5)
BsoBI 301 CTCGAG C^[CT]CG[GA]G
BspMI 475 ACCTGC ACCTGC(4/8)
BsrBI 156 CCGCTC CCGCTC(-3/-3)
Bsu36I 49 CCTCAGG CC^T[ACGT]AGG
DpnII 101 GATC ^GATC
EarI 25 GAAGAG CTCTTC(1/4)
Eco81I 49 CCTCAGG CC^T[ACGT]AGG
EcoNI 121 CCTCCTCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT14I 94 CCTTGG C^C[AT][AT]GG
HapII 459 CCGG C^CGG
HpaII 459 CCGG C^CGG
Ksp632I 25 GAAGAG CTCTTC(1/4)
MaeI 277 CTAG C^TAG
MaeIII 338 GTCAC ^GT[ACGT]AC
MboI 101 GATC ^GATC
MspI 459 CCGG C^CGG
NciI 458 CCCGG CC^[GC]GG
NdeII 101 GATC ^GATC
PaeR7I 301 CTCGAG C^TCGAG
PflMI 348 CCAGCGGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PstI 178 CTGCAG CTGCA^G
SacI 400 GAGCTC GAGCT^C
Sau3AI 101 GATC ^GATC
SexAI 200 ACCTGGT A^CC[AT]GGT
SfcI 178 CTGCAG C^T[GA][CT]AG
StyI 94 CCTTGG C^C[AT][AT]GG
TseI 414 GCTGC G^C[AT]GC
Tsp45I 338 GTCAC ^GT[GC]AC
TspRI 343 CAGTG CA[GC]TG[ACGT][ACGT]^
Van91I 348 CCAGCGGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 301 CTCGAG C^TCGAG
XmnI 187 GAACTCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 15 enzymes) #
###################################
AluI 317 AGCT AG^CT
401 AGCT
BanII 42 GGGCCC G[GA]GC[CT]^C
400 GAGCTC
BmyI 42 GGGCCC G[AGT]GC[ACT]^C
400 GAGCTC
Bsp1286I 42 GGGCCC G[AGT]GC[ACT]^C
400 GAGCTC
BsrI 216 CCAGT ACTGG(1/-1)
342 CCAGT
Cac8I 65 GCTGGC GC[ACGT]^[ACGT]GC
347 GCCAGC
Fnu4HI 224 GCCGC GC^[ACGT]GC
414 GCTGC
HphI 34 GGTGA GGTGA(8/7)
339 TCACC
ItaI 224 GCCGC GC^[ACGT]GC
414 GCTGC
MboII 22 GAAGA GAAGA(8/7)
25 GAAGA
MspA1I 349 CAGCGG C[AC]G^C[GT]G
364 CAGCGG
MwoI 171 GCTCTCCCTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
366 GCGGTCAGAGC
SmlI 270 CTCAAG C^T[CT][GA]AG
301 CTCGAG
TaqI 302 TCGA T^CGA
438 TCGA
TthHB8I 302 TCGA T^CGA
438 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 25 enzymes) #
#########################################
AciI AvaII BpmI BsaJI BseRI
BsiYI BslI BsmFI BssKI BstF5I
BstNI Cfr13I DdeI DraII EcoO109I
EcoRII FauI FokI HaeIII MnlI
MvaI NlaIV PpuMI Sau96I ScrFI