################################## # NO CUT ENZYMES ( 146 enzymes) # ################################## Acc65I AccI AccIII AclI AcsI AflII AhdI Alw44I AlwNI AocI Aor51HI ApaI ApaLI ApoI AscI Asp700I Asp718I AspEI AspI AvaI AviII AvrII BamHI BanI BbeI BbrPI BbsI BbvCI BcgI BciVI BclI BfaI BfrI BglI BglII BlnI BlpI BpmI Bpu1102I BpuAI BsaAI BsaI BseAI BsiEI BsiWI BsmAI BsmBI BsmI BsoBI Bsp1407I BspEI BspLU11I BspMI BsrBI BsrDI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstYI BstZ17I Bsu36I CelII CpoI DrdI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT22I FbaI FseI FspI HaeII HincII HindII HindIII HpaI KasI KpnI KspI MaeI MflI MluI MroI NaeI NarI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PmaCI PmeI PmlI PshAI Psp1406I PstI PvuI PvuII RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfcI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI Sse8387I SspBI SspI StuI SwaI Tth111I Van91I XbaI XcmI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 38 enzymes) # ################################### AatII 328 GACGTC GACGT^C AcyI 328 GACGTC G[GA]^CG[CT]C AflIII 707 ACACGT A^C[GA][CT]GT AgeI 512 ACCGGT A^CCGGT AlwI 577 GGATC GGATC(4/5) AseI 970 ATTAAT AT^TAAT AsnI 970 ATTAAT AT^TAAT AspHI 1106 GAGCAC G[AT]GC[AT]^C BalI 702 TGGCCA TGG^CCA BanII 189 GGGCTC G[GA]GC[CT]^C BsaBI 578 GATCAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaHI 328 GACGTC G[GA]^CG[CT]C BsaWI 512 ACCGGT [AT]^CCGG[AT] BsiHKAI 1106 GAGCAC G[AT]GC[AT]^C BspDI 547 ATCGAT AT^CGAT BstXI 167 CCAACGCCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG ClaI 547 ATCGAT AT^CGAT DdeI 258 CTTAG C^T[ACGT]AG DraI 210 TTTAAA TTT^AAA DraIII 713 CACACAGTG CAC[ACGT][ACGT][ACGT]^GTG EaeI 702 TGGCCA [CT]^GGCC[GA] EarI 247 CTCTTC CTCTTC(1/4) Hin1I 328 GACGTC G[GA]^CG[CT]C Ksp632I 247 CTCTTC CTCTTC(1/4) MamI 578 GATCAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MfeI 960 CAATTG C^AATTG MluNI 702 TGGCCA TGG^CCA MscI 702 TGGCCA TGG^CCA MslI 1134 CATGAATATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MspA1I 696 CAGCGG C[AC]G^C[GT]G MunI 960 CAATTG C^AATTG NdeI 1093 CATATG CA^TATG NspI 81 GCATGC [GA]CATG^[CT] PinAI 512 ACCGGT A^CCGGT PshBI 970 ATTAAT AT^TAAT SphI 81 GCATGC GCATG^C TaqI 548 TCGA T^CGA TthHB8I 548 TCGA T^CGA ################################### # TWO CUT ENZYMES ( 25 enzymes) # ################################### AccII 304 CGCG CG^CG 385 CGCG BcnI 310 CCGGG CC^[GC]GG 977 CCCGG BmyI 189 GGGCTC G[AGT]GC[ACT]^C 1106 GAGCAC BseRI 128 CTCCTC GAGGAG(10/8) 192 CTCCTC BsgI 133 CTGCAC GTGCAG(16/14) 991 GTGCAG Bsp1286I 189 GGGCTC G[AGT]GC[ACT]^C 1106 GAGCAC BspHI 648 TCATGA T^CATGA 857 TCATGA BsrFI 102 GCCGGT [GA]^CCGG[CT] 512 ACCGGT BsrI 420 ACTGG ACTGG(1/-1) 1008 CCAGT BstUI 304 CGCG CG^CG 385 CGCG CfoI 4 GCGC GCG^C 386 GCGC Cfr10I 102 GCCGGT [GA]^CCGG[CT] 512 ACCGGT EcoT14I 173 CCATGG C^C[AT][AT]GG 1061 CCATGG HgaI 205 GACGC GACGC(5/10) 813 GCGTC HhaI 4 GCGC GCG^C 386 GCGC HinP1I 4 GCGC G^CGC 386 GCGC MaeII 329 ACGT A^CGT 709 ACGT MvnI 304 CGCG CG^CG 385 CGCG NciI 310 CCGGG CC^[GC]GG 977 CCCGG NcoI 173 CCATGG C^CATGG 1061 CCATGG PleI 938 GACTC GAGTC(4/5) 343 GAGTC PpuMI 74 GGGACCC [GA]G^G[AT]CC[CT] 1113 AGGACCT RcaI 648 TCATGA T^CATGA 857 TCATGA StyI 173 CCATGG C^C[AT][AT]GG 1061 CCATGG TfiI 381 GATTC G^A[AT]TC 435 GATTC ######################################### # 3 OR MORE CUT ENZYMES ( 49 enzymes) # ######################################### AciI AfaI AluI AvaII BbvI BsaJI BsiYI BslI BsmFI BssKI BstF5I BstNI Cac8I Cfr13I DpnII DraII DsaI EcoO109I EcoRII FauI Fnu4HI FokI HaeIII HapII HinfI HpaII HphI ItaI MaeIII MboI MboII MnlI MseI MspI MvaI MwoI NdeII NlaIII NlaIV RsaI Sau3AI Sau96I ScrFI SfaNI Tru9I TseI Tsp45I Tsp509I TspRI