##################################
# NO CUT ENZYMES ( 197 enzymes) #
##################################
AatII Acc65I AccII AccIII AciI
AclI AcsI AcyI AflII AflIII
AgeI AhdI Alw44I AlwNI AocI
Aor51HI ApaI ApaLI ApoI AscI
AseI AsnI Asp700I Asp718I AspEI
AspHI AspI AvaI AviII AvrII
BamHI BanI BanII BbeI BbrPI
BbsI BbvCI BbvI BcgI BciVI
BclI BcnI BfrI BglI BglII
BlnI BlpI BmyI Bpu1102I BpuAI
BsaAI BsaBI BsaHI BsaWI BseAI
BseRI BsgI BsiEI BsiHKAI BsiWI
BsmBI BsmFI BsoBI Bsp1286I Bsp1407I
BspDI BspEI BspHI BspLU11I BspMI
BsrBI BsrDI BsrFI BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstUI BstXI BstZ17I Bsu36I
CelII CfoI Cfr10I ClaI CpoI
DraI DraII DraIII DrdI DsaI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoO109I EcoO65I EcoRI EcoT22I
FauI FbaI Fnu4HI FseI FspI
HaeII HapII HgaI HhaI Hin1I
HinP1I HincII HindII HindIII HpaI
HpaII ItaI KasI KpnI KspI
MaeIII MamI MfeI MluI MroI
MseI MslI MspA1I MspI MunI
MvnI NaeI NarI NciI NcoI
NdeI NgoMIV NheI NlaIV NotI
NruI NsiI NspV PacI PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PvuI PvuII RcaI RsrII SacI
SacII SalI SapI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmaI SmlI SnaBI SpeI SphI
Sse8387I SspBI StuI SwaI TfiI
Tru9I TseI Tsp45I Tsp509I Tth111I
Van91I XbaI XcmI XhoI XmaCI
XmaI XmnI
###################################
# ONE CUT ENZYMES ( 34 enzymes) #
###################################
AccI 472 GTCTAC GT^[AC][GT]AC
AfaI 376 GTAC GT^AC
AluI 436 AGCT AG^CT
AlwI 5 GATCC GGATC(4/5)
AvaII 284 GGACC G^G[AT]CC
BalI 29 TGGCCA TGG^CCA
BfaI 274 CTAG C^TAG
BpmI 357 CTCCAG CTGGAG(16/14)
BsaI 389 GGTCTC GGTCTC(1/5)
BsmAI 390 GTCTC GTCTC(1/5)
BsmI 163 GCATTC GAATGC(1/-1)
BstYI 4 AGATCC [GA]^GATC[CT]
Cac8I 106 GCCAGC GC[ACGT]^[ACGT]GC
EaeI 29 TGGCCA [CT]^GGCC[GA]
EarI 429 GAAGAG CTCTTC(1/4)
EcoNI 262 CCTGGGGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV 217 GATATC GAT^ATC
EcoT14I 385 CCAAGG C^C[AT][AT]GG
HphI 307 GGTGA GGTGA(8/7)
Ksp632I 429 GAAGAG CTCTTC(1/4)
MaeI 274 CTAG C^TAG
MaeII 443 ACGT A^CGT
MflI 4 AGATCC [GA]^GATC[CT]
MluNI 29 TGGCCA TGG^CCA
MscI 29 TGGCCA TGG^CCA
MwoI 480 GCCTGGACTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NspI 476 ACATGC [GA]CATG^[CT]
PstI 487 CTGCAG CTGCA^G
RsaI 376 GTAC GT^AC
SfaNI 91 GATGC GCATC(5/9)
SfcI 487 CTGCAG C^T[GA][CT]AG
SspI 124 AATATT AAT^ATT
StyI 385 CCAAGG C^C[AT][AT]GG
XhoII 4 AGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
BsaJI 262 CCTGGG C^C[ACGT][ACGT]GG
385 CCAAGG
BssKI 262 CCTGG ^CC[ACGT]GG
481 CCTGG
BstNI 262 CCTGG CC^[AT]GG
481 CCTGG
Cfr13I 284 GGACC G^G[ACGT]CC
319 GGGCC
DpnII 5 GATC ^GATC
297 GATC
EcoRII 262 CCTGG ^CC[AT]GG
481 CCTGG
HaeIII 30 GGCC GG^CC
320 GGCC
HinfI 250 GAGTC G^A[ACGT]TC
355 GACTC
MboI 5 GATC ^GATC
297 GATC
MvaI 262 CCTGG CC^[AT]GG
481 CCTGG
NdeII 5 GATC ^GATC
297 GATC
NlaIII 137 CATG CATG^
477 CATG
PleI 355 GACTC GAGTC(4/5)
250 GAGTC
Sau3AI 5 GATC ^GATC
297 GATC
Sau96I 284 GGACC G^G[ACGT]CC
319 GGGCC
ScrFI 262 CCTGG CC^[ACGT]GG
481 CCTGG
TaqI 246 TCGA T^CGA
299 TCGA
TspRI 156 CAGTG CA[GC]TG[ACGT][ACGT]^
490 CAGTG
TthHB8I 246 TCGA T^CGA
299 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 8 enzymes) #
#########################################
BsiYI BslI BsrI BstF5I DdeI
FokI MboII MnlI