Restriction map for
pF1KB0942
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# NO CUT ENZYMES ( 150 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcyI AgeI AhdI AlwI AocI
Aor51HI ApaI AscI AseI AsnI
Asp718I AspEI AspI AviII AvrII
BalI BamHI BbeI BbrPI BbsI
BbvCI BcgI BciVI BclI BglI
BglII BlnI BlpI Bpu1102I BpuAI
BsaAI BsaBI BsaHI BsaI BsaWI
BseAI BsgI BsiEI BsiWI BsmAI
BsmBI BspDI BspEI BsrFI BssHII
Bst1107I BstAPI BstBI BstXI BstYI
BstZ17I Bsu36I CelII CfoI Cfr10I
ClaI CpoI DdeI DraIII DrdI
DsaI EaeI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoNI EcoRI
EcoT14I EcoT22I FauI FbaI FseI
FspI HaeII HgaI HhaI Hin1I
HinP1I HincII HindII HpaI KasI
KpnI KspI MamI MfeI MflI
MluI MluNI MroI MscI MunI
NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI RsrII SacI SacII
SalI SapI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SphI Sse8387I SspI StuI
StyI SwaI Tth111I Van91I XbaI
XcmI XhoI XhoII XmaCI XmaI
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# ONE CUT ENZYMES ( 57 enzymes) #
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AccII 1054 CGCG CG^CG
AflII 826 CTTAAG C^TTAAG
Alw44I 1012 GTGCAC G^TGCAC
ApaLI 1012 GTGCAC G^TGCAC
AspHI 1012 GTGCAC G[AT]GC[AT]^C
AvaI 61 CCCGAG C^[CT]CG[GA]G
BanI 595 GGCACC G^G[CT][GA]CC
BbvI 946 GCTGC GCAGC(8/12)
BcnI 54 CCCGG CC^[GC]GG
BfrI 826 CTTAAG C^TTAAG
BsaJI 62 CCGAGG C^C[ACGT][ACGT]GG
BseRI 524 GAGGAG GAGGAG(10/8)
BsiHKAI 1012 GTGCAC G[AT]GC[AT]^C
BsmFI 516 GTCCC GGGAC(10/14)
BsoBI 61 CCCGAG C^[CT]CG[GA]G
Bsp1407I 48 TGTACA T^GTACA
BspHI 729 TCATGA T^CATGA
BspLU11I 749 ACATGT A^CATGT
BspMI 27 GCAGGT ACCTGC(4/8)
BsrBI 296 GAGCGG CCGCTC(-3/-3)
BsrDI 84 GCAATG GCAATG(2/0)
BsrGI 48 TGTACA T^GTACA
BsrI 169 ACTGG ACTGG(1/-1)
BssSI 79 CACGAG CACGAG(-5/-1)
BstEII 375 GGTGACC G^GT[ACGT]ACC
BstPI 375 GGTGACC G^GT[ACGT]ACC
BstUI 1054 CGCG CG^CG
Cac8I 67 GCTTGC GC[ACGT]^[ACGT]GC
DpnII 467 GATC ^GATC
DraI 721 TTTAAA TTT^AAA
DraII 29 AGGTCCT [GA]G^G[ACGT]CC[CT]
EcoO109I 29 AGGTCCT [GA]G^G[ACGT]CC[CT]
EcoO65I 375 GGTGACC G^GT[ACGT]ACC
EcoRV 1101 GATATC GAT^ATC
HapII 55 CCGG C^CGG
HindIII 823 AAGCTT A^AGCTT
HpaII 55 CCGG C^CGG
MboI 467 GATC ^GATC
MspA1I 944 CAGCTG C[AC]G^C[GT]G
MspI 55 CCGG C^CGG
MvnI 1054 CGCG CG^CG
MwoI 118 GCTCACACTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NciI 54 CCCGG CC^[GC]GG
NdeII 467 GATC ^GATC
NspI 749 ACATGT [GA]CATG^[CT]
PpuMI 29 AGGTCCT [GA]G^G[AT]CC[CT]
PstI 947 CTGCAG CTGCA^G
PvuII 944 CAGCTG CAG^CTG
RcaI 729 TCATGA T^CATGA
Sau3AI 467 GATC ^GATC
SfcI 947 CTGCAG C^T[GA][CT]AG
SmlI 826 CTTAAG C^T[CT][GA]AG
SpeI 702 ACTAGT A^CTAGT
SspBI 48 TGTACA T^GTACA
TaqI 796 TCGA T^CGA
TseI 946 GCTGC G^C[AT]GC
TthHB8I 796 TCGA T^CGA
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# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AflIII 749 ACATGT A^C[GA][CT]GT
889 ACGTGT
Asp700I 184 GAAACAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
931 GAAAGCCTTC
AvaII 30 GGTCC G^G[AT]CC
515 GGTCC
BanII 58 GAGCCC G[GA]GC[CT]^C
227 GGGCTC
BpmI 941 CTCCAG CTGGAG(16/14)
662 CTGGAG
BsiYI 651 CCTTGCAGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
1109 CCAAGAAGAGG
BslI 651 CCTTGCAGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
1109 CCAAGAAGAGG
BsmI 340 GAATGC GAATGC(1/-1)
472 GCATTC
BstF5I 584 CATCC GGATG(2/0)
324 GGATG
BstNI 881 CCAGG CC^[AT]GG
1000 CCTGG
EarI 1113 GAAGAG CTCTTC(1/4)
1143 GAAGAG
EcoRII 881 CCAGG ^CC[AT]GG
1000 CCTGG
Fnu4HI 71 GCGGC GC^[ACGT]GC
946 GCTGC
FokI 584 CATCC GGATG(9/13)
324 GGATG
HaeIII 411 GGCC GG^CC
1096 GGCC
ItaI 71 GCGGC GC^[ACGT]GC
946 GCTGC
Ksp632I 1113 GAAGAG CTCTTC(1/4)
1143 GAAGAG
MaeII 445 ACGT A^CGT
889 ACGT
MvaI 881 CCAGG CC^[AT]GG
1000 CCTGG
TfiI 450 GAATC G^A[AT]TC
697 GATTC
XmnI 184 GAAACAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
931 GAAAGCCTTC
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# 3 OR MORE CUT ENZYMES ( 30 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwNI
ApoI BfaI BmyI Bsp1286I BssKI
Cfr13I HinfI HphI MaeI MaeIII
MboII MnlI MseI MslI NlaIII
NlaIV PleI RsaI Sau96I ScrFI
SfaNI Tru9I Tsp45I Tsp509I TspRI