Restriction map for
pF1KB0950
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# NO CUT ENZYMES ( 110 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AflII AflIII AhdI Alw44I
AlwNI ApaI ApaLI ApoI AscI
AseI AsnI Asp700I Asp718I AspEI
AspHI AvrII BalI BamHI BbrPI
BbvCI BciVI BclI BfrI BlnI
BlpI BpmI Bpu1102I BsaBI BsaI
BseAI BsgI BsiHKAI BsiWI BsmI
Bsp1407I BspDI BspEI BspHI BspLU11I
BsrDI BsrGI BssHII Bst1107I BstBI
BstZ17I CelII ClaI CpoI DraI
DrdI Eam1105I EcoRI EcoRV EcoT22I
FbaI HindIII HpaI KpnI KspI
MamI MfeI MluI MluNI MroI
MscI MunI NdeI NruI NsiI
NspV PacI PaeR7I PleI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI RcaI RsrII SacI SacII
SalI SapI SbfI ScaI SfiI
SfuI SgrAI SmaI SmlI SpeI
Sse8387I SspBI SspI SwaI Tsp509I
XbaI XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 63 enzymes) #
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AgeI 1034 ACCGGT A^CCGGT
AlwI 687 GATCC GGATC(4/5)
Aor51HI 291 AGCGCT AGC^GCT
AspI 781 GACGCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 799 TGCGCA TGC^GCA
BbeI 650 GGCGCC GGCGC^C
BbsI 57 GTCTTC GAAGAC(2/6)
BcgI 866 GCAACGGGCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BcnI 90 CCGGG CC^[GC]GG
BglII 983 AGATCT A^GATCT
BpuAI 57 GTCTTC GAAGAC(2/6)
BsaAI 880 TACGTA [CT]AC^GT[GA]
BsaWI 1034 ACCGGT [AT]^CCGG[AT]
BseRI 499 GAGGAG GAGGAG(10/8)
BsmAI 659 GAGAC GTCTC(1/5)
BsmBI 659 GAGACG CGTCTC(1/5)
BspMI 116 ACCTGC ACCTGC(4/8)
BssSI 1072 CACGAG CACGAG(-5/-1)
BstAPI 446 GCATGAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 297 GGTCACC G^GT[ACGT]ACC
BstPI 297 GGTCACC G^GT[ACGT]ACC
BstYI 983 AGATCT [GA]^GATC[CT]
DraIII 1072 CACGAGGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 265 CGGCCG C^GGCCG
EarI 224 CTCTTC CTCTTC(1/4)
EclXI 265 CGGCCG C^GGCCG
Eco47III 291 AGCGCT AGC^GCT
Eco52I 265 CGGCCG C^GGCCG
EcoO65I 297 GGTCACC G^GT[ACGT]ACC
FseI 997 GGCCGGCC GGCCGG^CC
FspI 799 TGCGCA TGC^GCA
HincII 892 GTTGAC GT[CT]^[GA]AC
HindII 892 GTTGAC GT[CT]^[GA]AC
HinfI 942 GAATC G^A[ACGT]TC
KasI 650 GGCGCC G^GCGCC
Ksp632I 224 CTCTTC CTCTTC(1/4)
MflI 983 AGATCT [GA]^GATC[CT]
MseI 959 TTAA T^TAA
MslI 904 CACGGCGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI 998 GCCGGC GCC^GGC
NarI 650 GGCGCC GG^CGCC
NciI 90 CCGGG CC^[GC]GG
NcoI 845 CCATGG C^CATGG
NgoMIV 998 GCCGGC G^CCGGC
NheI 1164 GCTAGC G^CTAGC
NotI 264 GCGGCCGC GC^GGCCGC
NspI 554 GCATGC [GA]CATG^[CT]
PflFI 781 GACGCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 312 CCAGCGATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 1034 ACCGGT A^CCGGT
PpuMI 184 GGGACCC [GA]G^G[AT]CC[CT]
PvuII 456 CAGCTG CAG^CTG
SexAI 1096 ACCAGGT A^CC[AT]GGT
SfoI 650 GGCGCC GGC^GCC
SnaBI 880 TACGTA TAC^GTA
SphI 554 GCATGC GCATG^C
StuI 167 AGGCCT AGG^CCT
TfiI 942 GAATC G^A[AT]TC
Tru9I 959 TTAA T^TAA
Tsp45I 298 GTCAC ^GT[GC]AC
Tth111I 781 GACGCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 312 CCAGCGATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 983 AGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AccII 613 CGCG CG^CG
695 CGCG
AcyI 650 GGCGCC G[GA]^CG[CT]C
781 GACGCC
AocI 340 CCTGAGG CC^T[ACGT]AGG
991 CCTGAGG
AvaI 520 CCCGAG C^[CT]CG[GA]G
874 CTCGGG
AvaII 185 GGACC G^G[AT]CC
711 GGACC
BanII 67 GAGCCC G[GA]GC[CT]^C
871 GGGCTC
BglI 763 GCCCTCATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
844 GCCATGGTGGC
BsaHI 650 GGCGCC G[GA]^CG[CT]C
781 GACGCC
BsiEI 265 CGGCCG CG[GA][CT]^CG
609 CGGTCG
BsoBI 520 CCCGAG C^[CT]CG[GA]G
874 CTCGGG
BsrBI 433 GAGCGG CCGCTC(-3/-3)
1135 GAGCGG
BstUI 613 CGCG CG^CG
695 CGCG
BstXI 839 CCAAGGCCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
903 CCACGGCGATGG
Bsu36I 340 CCTGAGG CC^T[ACGT]AGG
991 CCTGAGG
DpnII 687 GATC ^GATC
984 GATC
Eco81I 340 CCTGAGG CC^T[ACGT]AGG
991 CCTGAGG
EcoNI 27 CCTAAGTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
404 CCTGGGCCAGG
HgaI 781 GACGC GACGC(5/10)
575 GCGTC
Hin1I 650 GGCGCC G[GA]^CG[CT]C
781 GACGCC
MboI 687 GATC ^GATC
984 GATC
MspA1I 268 CCGCTG C[AC]G^C[GT]G
456 CAGCTG
MvnI 613 CGCG CG^CG
695 CGCG
NdeII 687 GATC ^GATC
984 GATC
Sau3AI 687 GATC ^GATC
984 GATC
TaqI 750 TCGA T^CGA
1019 TCGA
TthHB8I 750 TCGA T^CGA
1019 TCGA
XcmI 95 CCAGGATGGGAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
839 CCAAGGCCATGGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 58 enzymes) #
#########################################
AciI AfaI AluI BanI BbvI
BfaI BmyI BsaJI BsiYI BslI
BsmFI Bsp1286I BsrFI BsrI BssKI
BstF5I BstNI Cac8I CfoI Cfr10I
Cfr13I DdeI DraII DsaI EaeI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HhaI
HinP1I HpaII HphI ItaI MaeI
MaeII MaeIII MboII MnlI MspI
MvaI MwoI NlaIII NlaIV PstI
RsaI Sau96I ScrFI SfaNI SfcI
StyI TseI TspRI