Restriction map for
pF1KB6633
##################################
# NO CUT ENZYMES ( 108 enzymes) #
##################################
AatII AccI AccIII AclI AcsI
AflII AflIII AgeI AhdI AlwNI
Aor51HI ApaI ApoI AscI AseI
AsnI Asp700I AspEI AspI AviII
AvrII BamHI BbrPI BcgI BfrI
BglI BglII BlnI BsaWI BseAI
BsmBI BsmI BspDI BspEI BspHI
BspLU11I BsrBI BsrDI BssSI Bst1107I
BstBI BstEII BstPI BstZ17I ClaI
CpoI DraI DrdI Eam1105I EarI
Eco47III EcoNI EcoO65I EcoRI EcoRV
EcoT22I FseI FspI HincII HindII
HindIII HpaI Ksp632I MfeI MluI
MroI MseI MunI NcoI NdeI
NheI NruI NsiI NspV PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PstI
PvuI PvuII RcaI RsrII SacI
SalI SapI SbfI SfcI SfiI
SfuI SmlI SnaBI SpeI SphI
Sse8387I SspI StuI SwaI Tru9I
Tth111I XhoI XmnI
###################################
# ONE CUT ENZYMES ( 59 enzymes) #
###################################
Acc65I 593 GGTACC G^GTACC
Alw44I 55 GTGCAC G^TGCAC
AocI 1226 CCTCAGG CC^T[ACGT]AGG
ApaLI 55 GTGCAC G^TGCAC
Asp718I 593 GGTACC G^GTACC
BalI 734 TGGCCA TGG^CCA
BbsI 976 GAAGAC GAAGAC(2/6)
BbvCI 196 CCTCAGC CCTCAGC(-5/-2)
BciVI 604 GGATAC GTATCC(6/5)
BclI 771 TGATCA T^GATCA
BlpI 171 GCTGAGC GC^T[ACGT]AGC
BpmI 329 CTGGAG CTGGAG(16/14)
Bpu1102I 171 GCTGAGC GC^T[ACGT]AGC
BpuAI 976 GAAGAC GAAGAC(2/6)
BsaBI 766 GATTATGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 631 GGTCTC GGTCTC(1/5)
BseRI 936 GAGGAG GAGGAG(10/8)
BsiWI 677 CGTACG C^GTACG
BsmAI 632 GTCTC GTCTC(1/5)
Bsp1407I 698 TGTACA T^GTACA
BsrGI 698 TGTACA T^GTACA
BstAPI 1015 GCATGTACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstYI 134 AGATCC [GA]^GATC[CT]
Bsu36I 1226 CCTCAGG CC^T[ACGT]AGG
CelII 171 GCTGAGC GC^T[ACGT]AGC
DraII 209 GGGACCC [GA]G^G[ACGT]CC[CT]
DraIII 775 CACAGGGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 299 CCGCGG C^C[GA][CT]GG
Eco81I 1226 CCTCAGG CC^T[ACGT]AGG
EcoO109I 209 GGGACCC [GA]G^G[ACGT]CC[CT]
EcoT14I 813 CCAAGG C^C[AT][AT]GG
FbaI 771 TGATCA T^GATCA
HgaI 670 GCGTC GACGC(5/10)
KpnI 593 GGTACC GGTAC^C
KspI 299 CCGCGG CCGC^GG
MamI 766 GATTATGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 134 AGATCC [GA]^GATC[CT]
MluNI 734 TGGCCA TGG^CCA
MscI 734 TGGCCA TGG^CCA
NotI 471 GCGGCCGC GC^GGCCGC
NspI 1015 GCATGT [GA]CATG^[CT]
PflMI 885 CCACAGTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 209 GGGACCC [GA]G^G[AT]CC[CT]
SacII 299 CCGCGG CCGC^GG
ScaI 527 AGTACT AGT^ACT
SgrAI 104 CGCCGGCG C[GA]^CCGG[CT]G
SmaI 213 CCCGGG CCC^GGG
SspBI 698 TGTACA T^GTACA
StyI 813 CCAAGG C^C[AT][AT]GG
TaqI 388 TCGA T^CGA
TfiI 1162 GAATC G^A[AT]TC
Tsp509I 1239 AATT ^AATT
TthHB8I 388 TCGA T^CGA
Van91I 885 CCACAGTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 394 TCTAGA T^CTAGA
XcmI 844 CCAAGATATTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 134 AGATCC [GA]^GATC[CT]
XmaCI 213 CCCGGG C^CCGGG
XmaI 213 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AspHI 19 GTGCTC G[AT]GC[AT]^C
55 GTGCAC
AvaI 28 CTCGGG C^[CT]CG[GA]G
213 CCCGGG
AvaII 14 GGACC G^G[AT]CC
210 GGACC
BanII 180 GGGCTC G[GA]GC[CT]^C
398 GAGCCC
BfaI 52 CTAG C^TAG
395 CTAG
BsaAI 675 CACGTA [CT]AC^GT[GA]
679 TACGTG
BsiEI 317 CGGCCG CG[GA][CT]^CG
472 CGGCCG
BsiHKAI 19 GTGCTC G[AT]GC[AT]^C
55 GTGCAC
BsoBI 28 CTCGGG C^[CT]CG[GA]G
213 CCCGGG
BssHII 47 GCGCGC G^CGCGC
420 GCGCGC
BstXI 479 CCAGCCGCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1145 CCAGCTACCTGG
EagI 317 CGGCCG C^GGCCG
472 CGGCCG
EclXI 317 CGGCCG C^GGCCG
472 CGGCCG
Eco52I 317 CGGCCG C^GGCCG
472 CGGCCG
HphI 335 TCACC GGTGA(8/7)
1243 TCACC
MaeI 52 CTAG C^TAG
395 CTAG
MaeII 676 ACGT A^CGT
680 ACGT
MboII 687 GAAGA GAAGA(8/7)
976 GAAGA
MslI 675 CACGTACGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1216 CATGCGTATG
MspA1I 299 CCGCGG C[AC]G^C[GT]G
338 CCGCTG
PleI 332 GAGTC GAGTC(4/5)
1041 GAGTC
SexAI 641 ACCTGGT A^CC[AT]GGT
1151 ACCTGGT
SfaNI 710 GATGC GCATC(5/9)
922 GATGC
#########################################
# 3 OR MORE CUT ENZYMES ( 68 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI BanI BbeI BbvI BcnI
BmyI BsaHI BsaJI BsgI BsiYI
BslI BsmFI Bsp1286I BspMI BsrFI
BsrI BssKI BstF5I BstNI BstUI
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII EaeI EcoRII FauI Fnu4HI
FokI HaeII HaeIII HapII HhaI
Hin1I HinP1I HinfI HpaII ItaI
KasI MaeIII MboI MnlI MspI
MvaI MvnI MwoI NaeI NarI
NciI NdeII NgoMIV NlaIII NlaIV
RsaI Sau3AI Sau96I ScrFI SfoI
TseI Tsp45I TspRI