##################################
# NO CUT ENZYMES (  180 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AcsI        AcyI        AfaI        AflII       AflIII      
AgeI        AhdI        Alw44I      AlwI        AocI        
Aor51HI     ApaI        ApaLI       ApoI        AscI        
AseI        AsnI        Asp718I     AspEI       AspI        
AvaI        AviII       AvrII       BamHI       BanI        
BbeI        BbrPI       BbsI        BbvCI       BcgI        
BciVI       BclI        BfaI        BfrI        BglI        
BglII       BlnI        BlpI        Bpu1102I    BpuAI       
BsaAI       BsaBI       BsaHI       BsaI        BsaWI       
BseAI       BsgI        BsiEI       BsiWI       BsmAI       
BsmBI       BsmI        BsoBI       Bsp1407I    BspDI       
BspEI       BspHI       BspLU11I    BsrDI       BsrFI       
BsrGI       BssHII      BssSI       Bst1107I    BstBI       
BstEII      BstPI       BstXI       BstYI       BstZ17I     
Bsu36I      CelII       Cfr10I      ClaI        CpoI        
DdeI        DpnII       DraI        DraIII      DrdI        
DsaI        EagI        Eam1105I    EarI        EclXI       
Eco47III    Eco52I      Eco81I      EcoNI       EcoO65I     
EcoRI       EcoRV       EcoT14I     EcoT22I     FbaI        
FseI        FspI        HgaI        Hin1I       HincII      
HindII      HindIII     HpaI        KasI        KpnI        
Ksp632I     KspI        MaeI        MaeII       MamI        
MboI        MfeI        MflI        MluI        MroI        
MseI        MslI        MunI        NaeI        NarI        
NcoI        NdeI        NdeII       NgoMIV      NheI        
NotI        NruI        NsiI        NspV        PacI        
PaeR7I      PflFI       PflMI       PinAI       PmaCI       
PmeI        PmlI        PshAI       PshBI       Psp1406I    
PvuI        RcaI        RsaI        RsrII       SacII       
SalI        SapI        Sau3AI      SbfI        ScaI        
SexAI       SfiI        SfoI        SfuI        SgrAI       
SmaI        SmlI        SnaBI       SpeI        Sse8387I    
SspBI       SspI        StuI        StyI        SwaI        
Tru9I       Tsp509I     Tth111I     Van91I      XbaI        
XcmI        XhoI        XhoII       XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   22 enzymes) #
###################################

AccII         415  CGCG                 CG^CG
Asp700I        99  GAAGAAGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BalI          204  TGGCCA               TGG^CCA
BsmFI         213  GTCCC                GGGAC(10/14)
BsrBI          40  GAGCGG               CCGCTC(-3/-3)
BstAPI        378  GCAGGACCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI         415  CGCG                 CG^CG
HphI          479  TCACC                GGTGA(8/7)
MaeIII        478  GTCAC                ^GT[ACGT]AC
MluNI         204  TGGCCA               TGG^CCA
MscI          204  TGGCCA               TGG^CCA
MvnI          415  CGCG                 CG^CG
NspI          293  GCATGC               [GA]CATG^[CT]
PleI          433  GACTC                GAGTC(4/5)
PstI          468  CTGCAG               CTGCA^G
PvuII         545  CAGCTG               CAG^CTG
SacI          313  GAGCTC               GAGCT^C
SfcI          468  CTGCAG               C^T[GA][CT]AG
SphI          293  GCATGC               GCATG^C
TfiI          176  GATTC                G^A[AT]TC
Tsp45I        478  GTCAC                ^GT[GC]AC
XmnI           99  GAAGAAGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   20 enzymes) #
###################################

AlwNI         319  CAGAGCCTG            CAG[ACGT][ACGT][ACGT]^CTG
              379  CAGGACCTG           
BanII           4  GGGCTC               G[GA]GC[CT]^C
              313  GAGCTC              
BcnI          482  CCGGG                CC^[GC]GG
              499  CCGGG               
BseRI         358  CTCCTC               GAGGAG(10/8)
              334  GAGGAG              
BspMI         383  ACCTGC               ACCTGC(4/8)
              491  ACCTGC              
DraII         380  AGGACCT              [GA]G^G[ACGT]CC[CT]
              419  AGGTCCT             
EaeI          204  TGGCCA               [CT]^GGCC[GA]
              344  TGGCCG              
EcoO109I      380  AGGACCT              [GA]G^G[ACGT]CC[CT]
              419  AGGTCCT             
FauI           42  GCGGG                CCCGC(4/6)
              309  GCGGG               
HaeII         338  AGCGCC               [GA]GCGC^[CT]
              472  AGCGCC              
HinfI         176  GATTC                G^A[ACGT]TC
              433  GACTC               
MspA1I        307  CAGCGG               C[AC]G^C[GT]G
              545  CAGCTG              
NciI          482  CCGGG                CC^[GC]GG
              499  CCGGG               
NlaIII         60  CATG                 CATG^
              294  CATG                
NlaIV           5  GGCTCC               GG[ACGT]^[ACGT]CC
              512  GGCTCC              
PpuMI         380  AGGACCT              [GA]G^G[AT]CC[CT]
              419  AGGTCCT             
SfaNI         458  GCATC                GCATC(5/9)
              503  GCATC               
TaqI          122  TCGA                 T^CGA
              506  TCGA                
TspRI         395  CACTG                CA[GC]TG[ACGT][ACGT]^
              451  CACTG               
TthHB8I       122  TCGA                 T^CGA
              506  TCGA                


#########################################
# 3 OR MORE CUT ENZYMES (   36 enzymes) #
#########################################

AciI        AluI        AspHI       AvaII       BbvI        
BmyI        BpmI        BsaJI       BsiHKAI     BsiYI       
BslI        Bsp1286I    BsrI        BssKI       BstF5I      
BstNI       Cac8I       CfoI        Cfr13I      EcoRII      
Fnu4HI      FokI        HaeIII      HapII       HhaI        
HinP1I      HpaII       ItaI        MboII       MnlI        
MspI        MvaI        MwoI        Sau96I      ScrFI       
TseI