Restriction map for
pF1KB0955
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# NO CUT ENZYMES ( 163 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AfaI AflII AflIII AgeI
AhdI Alw44I AlwI AlwNI AocI
Aor51HI ApaLI AscI AseI AsnI
Asp700I Asp718I AspEI AspHI AspI
AvaII AviII AvrII BalI BamHI
BbrPI BbvCI BcgI BciVI BfaI
BfrI BglI BglII BlnI BlpI
BpmI Bpu1102I BsaAI BsaBI BsaI
BsaWI BseAI BsgI BsiEI BsiHKAI
BsiWI BsmAI BsmBI BsmI Bsp1407I
BspDI BspEI BspHI BspLU11I BsrDI
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstUI BstXI BstYI BstZ17I
Bsu36I CelII ClaI CpoI DraI
DraIII DrdI DsaI EaeI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoNI EcoRI EcoRV EcoT14I FseI
FspI HincII HindII HindIII HinfI
HpaI KpnI KspI MaeI MaeII
MamI MfeI MflI MluI MluNI
MroI MscI MseI MslI MunI
MvnI NcoI NdeI NheI NlaIII
NotI NruI NspI NspV PacI
PflFI PinAI PleI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PvuI PvuII RcaI RsaI RsrII
SacI SacII SalI SbfI ScaI
SexAI SfiI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI StyI SwaI TfiI
Tru9I Tth111I XbaI XcmI XhoII
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 48 enzymes) #
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AcsI 301 GAATTT [GA]^AATT[CT]
AcyI 201 GGCGCC G[GA]^CG[CT]C
ApoI 301 GAATTT [GA]^AATT[CT]
AvaI 154 CTCGAG C^[CT]CG[GA]G
BbeI 201 GGCGCC GGCGC^C
BbsI 456 GAAGAC GAAGAC(2/6)
BclI 468 TGATCA T^GATCA
BcnI 111 CCGGG CC^[GC]GG
BpuAI 456 GAAGAC GAAGAC(2/6)
BsaHI 201 GGCGCC G[GA]^CG[CT]C
BsaJI 425 CCCTGG C^C[ACGT][ACGT]GG
BsmFI 128 GTCCC GGGAC(10/14)
BsoBI 154 CTCGAG C^[CT]CG[GA]G
BspMI 47 ACCTGC ACCTGC(4/8)
BsrBI 377 CCGCTC CCGCTC(-3/-3)
BsrFI 358 GCCGGC [GA]^CCGG[CT]
BsrI 311 ACTGG ACTGG(1/-1)
BstEII 76 GGTGACC G^GT[ACGT]ACC
BstPI 76 GGTGACC G^GT[ACGT]ACC
Cfr10I 358 GCCGGC [GA]^CCGG[CT]
DpnII 469 GATC ^GATC
DraII 142 GGGGCCC [GA]G^G[ACGT]CC[CT]
EcoO109I 142 GGGGCCC [GA]G^G[ACGT]CC[CT]
EcoO65I 76 GGTGACC G^GT[ACGT]ACC
EcoT22I 445 ATGCAT ATGCA^T
FbaI 468 TGATCA T^GATCA
HaeII 201 GGCGCC [GA]GCGC^[CT]
HgaI 126 GCGTC GACGC(5/10)
Hin1I 201 GGCGCC G[GA]^CG[CT]C
HphI 76 GGTGA GGTGA(8/7)
KasI 201 GGCGCC G^GCGCC
MboI 469 GATC ^GATC
NaeI 358 GCCGGC GCC^GGC
NarI 201 GGCGCC GG^CGCC
NciI 111 CCGGG CC^[GC]GG
NdeII 469 GATC ^GATC
NgoMIV 358 GCCGGC G^CCGGC
NsiI 445 ATGCAT ATGCA^T
PaeR7I 154 CTCGAG C^TCGAG
PflMI 189 CCAGCCAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PstI 49 CTGCAG CTGCA^G
SapI 487 GAAGAGC GCTCTTC(1/4)
Sau3AI 469 GATC ^GATC
SfoI 201 GGCGCC GGC^GCC
SmlI 154 CTCGAG C^T[CT][GA]AG
Tsp509I 302 AATT ^AATT
Van91I 189 CCAGCCAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 154 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
ApaI 143 GGGCCC GGGCC^C
232 GGGCCC
BanI 201 GGCGCC G^G[CT][GA]CC
355 GGTGCC
BanII 143 GGGCCC G[GA]GC[CT]^C
232 GGGCCC
BmyI 143 GGGCCC G[AGT]GC[ACT]^C
232 GGGCCC
Bsp1286I 143 GGGCCC G[AGT]GC[ACT]^C
232 GGGCCC
BstF5I 57 GGATG GGATG(2/0)
429 GGATG
Cac8I 265 GCGGGC GC[ACGT]^[ACGT]GC
358 GCCGGC
CfoI 202 GCGC GCG^C
269 GCGC
FokI 57 GGATG GGATG(9/13)
429 GGATG
HapII 111 CCGG C^CGG
359 CCGG
HhaI 202 GCGC GCG^C
269 GCGC
HinP1I 202 GCGC G^CGC
269 GCGC
HpaII 111 CCGG C^CGG
359 CCGG
MaeIII 44 GTGAC ^GT[ACGT]AC
77 GTGAC
MspA1I 102 CAGCGG C[AC]G^C[GT]G
138 CAGCGG
MspI 111 CCGG C^CGG
359 CCGG
MwoI 101 GCAGCGGGAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
117 GCTATCCCTGC
SfaNI 63 GATGC GCATC(5/9)
444 GATGC
SfcI 49 CTGCAG C^T[GA][CT]AG
363 CTACAG
Tsp45I 44 GTGAC ^GT[GC]AC
77 GTGAC
#########################################
# 3 OR MORE CUT ENZYMES ( 27 enzymes) #
#########################################
AciI AluI BbvI BseRI BsiYI
BslI BssKI BstNI Cfr13I DdeI
EarI EcoRII FauI Fnu4HI HaeIII
ItaI Ksp632I MboII MnlI MvaI
NlaIV Sau96I ScrFI TaqI TseI
TspRI TthHB8I