################################## # NO CUT ENZYMES ( 136 enzymes) # ################################## AatII Acc65I AccI AclI AflII AgeI AhdI Aor51HI ApaI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvaI AvrII BamHI BanI BanII BbeI BbrPI BbsI BbvCI BcgI BciVI BclI BfrI BglI BglII BlnI BlpI Bpu1102I BpuAI BsaAI BsaBI BsiEI BsiWI BsmBI BsoBI Bsp1407I BspDI BspHI BspLU11I BspMI BsrBI BsrFI BsrGI BssSI Bst1107I BstAPI BstBI BstEII BstPI BstZ17I CelII Cfr10I ClaI CpoI DraI DraIII DrdI EagI Eam1105I EclXI Eco47III Eco52I EcoO65I EcoRI EcoRV EcoT22I FbaI FseI HincII HindII HpaI KasI KpnI KspI MamI MfeI MluI MunI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PshBI Psp1406I PvuI PvuII RcaI RsrII SacI SacII SalI SapI SbfI ScaI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI Tth111I Van91I XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 36 enzymes) # ################################### AccIII 125 TCCGGA T^CCGGA AcyI 844 GACGCC G[GA]^CG[CT]C AfaI 351 GTAC GT^AC AflIII 20 ACGTGT A^C[GA][CT]GT Alw44I 300 GTGCAC G^TGCAC ApaLI 300 GTGCAC G^TGCAC AspHI 300 GTGCAC G[AT]GC[AT]^C AviII 308 TGCGCA TGC^GCA BcnI 263 CCGGG CC^[GC]GG BsaHI 844 GACGCC G[GA]^CG[CT]C BseAI 125 TCCGGA T^CCGGA BsiHKAI 300 GTGCAC G[AT]GC[AT]^C BspEI 125 TCCGGA T^CCGGA BsrDI 337 CATTGC GCAATG(2/0) BssHII 53 GCGCGC G^CGCGC BstXI 1136 CCAAATGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG DsaI 65 CCGTGG C^C[GA][CT]GG FspI 308 TGCGCA TGC^GCA HaeII 88 AGCGCC [GA]GCGC^[CT] HgaI 844 GACGC GACGC(5/10) Hin1I 844 GACGCC G[GA]^CG[CT]C HindIII 448 AAGCTT A^AGCTT HphI 288 TCACC GGTGA(8/7) MaeII 20 ACGT A^CGT MroI 125 TCCGGA T^CCGGA MspA1I 1066 CAGCGG C[AC]G^C[GT]G NciI 263 CCGGG CC^[GC]GG NspI 999 GCATGT [GA]CATG^[CT] PpuMI 398 AGGTCCT [GA]G^G[AT]CC[CT] PshAI 1057 GACTGCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PstI 354 CTGCAG CTGCA^G RsaI 351 GTAC GT^AC SexAI 395 ACCAGGT A^CC[AT]GGT SfcI 354 CTGCAG C^T[GA][CT]AG TaqI 875 TCGA T^CGA TthHB8I 875 TCGA T^CGA ################################### # TWO CUT ENZYMES ( 21 enzymes) # ################################### AcsI 634 AAATTC [GA]^AATT[CT] 1037 AAATTT ApoI 634 AAATTC [GA]^AATT[CT] 1037 AAATTT BalI 521 TGGCCA TGG^CCA 756 TGGCCA BfaI 229 CTAG C^TAG 512 CTAG BsaWI 125 TCCGGA [AT]^CCGG[AT] 725 TCCGGT BsgI 167 CTGCAC GTGCAG(16/14) 359 GTGCAG BsmI 183 GAATGC GAATGC(1/-1) 246 GAATGC BstYI 910 GGATCT [GA]^GATC[CT] 1097 AGATCC EaeI 521 TGGCCA [CT]^GGCC[GA] 756 TGGCCA EcoNI 681 CCTCTATGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 1143 CCTGGCAGAGG EcoT14I 92 CCAAGG C^C[AT][AT]GG 759 CCAAGG MaeI 229 CTAG C^TAG 512 CTAG MflI 910 GGATCT [GA]^GATC[CT] 1097 AGATCC MluNI 521 TGGCCA TGG^CCA 756 TGGCCA MscI 521 TGGCCA TGG^CCA 756 TGGCCA MseI 899 TTAA T^TAA 1022 TTAA PleI 563 GACTC GAGTC(4/5) 665 GACTC StyI 92 CCAAGG C^C[AT][AT]GG 759 CCAAGG TfiI 722 GATTC G^A[AT]TC 764 GAATC Tru9I 899 TTAA T^TAA 1022 TTAA XhoII 910 GGATCT [GA]^GATC[CT] 1097 AGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 65 enzymes) # ######################################### AccII AciI AluI AlwI AlwNI AocI AvaII BbvI BmyI BpmI BsaI BsaJI BseRI BsiYI BslI BsmAI BsmFI Bsp1286I BsrI BssKI BstF5I BstNI BstUI Bsu36I Cac8I CfoI Cfr13I DdeI DpnII DraII EarI Eco81I EcoO109I EcoRII FauI Fnu4HI FokI HaeIII HapII HhaI HinP1I HinfI HpaII ItaI Ksp632I MaeIII MboI MboII MnlI MslI MspI MvaI MvnI MwoI NdeII NlaIII NlaIV Sau3AI Sau96I ScrFI SfaNI TseI Tsp45I Tsp509I TspRI