##################################
# NO CUT ENZYMES ( 136 enzymes) #
##################################
AatII Acc65I AccI AclI AflII
AgeI AhdI Aor51HI ApaI AscI
AseI AsnI Asp700I Asp718I AspEI
AspI AvaI AvrII BamHI BanI
BanII BbeI BbrPI BbsI BbvCI
BcgI BciVI BclI BfrI BglI
BglII BlnI BlpI Bpu1102I BpuAI
BsaAI BsaBI BsiEI BsiWI BsmBI
BsoBI Bsp1407I BspDI BspHI BspLU11I
BspMI BsrBI BsrFI BsrGI BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstZ17I CelII Cfr10I ClaI CpoI
DraI DraIII DrdI EagI Eam1105I
EclXI Eco47III Eco52I EcoO65I EcoRI
EcoRV EcoT22I FbaI FseI HincII
HindII HpaI KasI KpnI KspI
MamI MfeI MluI MunI NaeI
NarI NcoI NdeI NgoMIV NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PflMI PinAI PmaCI
PmeI PmlI PshBI Psp1406I PvuI
PvuII RcaI RsrII SacI SacII
SalI SapI SbfI ScaI SfiI
SfoI SfuI SgrAI SmaI SmlI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI SwaI Tth111I Van91I
XbaI XcmI XhoI XmaCI XmaI
XmnI
###################################
# ONE CUT ENZYMES ( 36 enzymes) #
###################################
AccIII 125 TCCGGA T^CCGGA
AcyI 844 GACGCC G[GA]^CG[CT]C
AfaI 351 GTAC GT^AC
AflIII 20 ACGTGT A^C[GA][CT]GT
Alw44I 300 GTGCAC G^TGCAC
ApaLI 300 GTGCAC G^TGCAC
AspHI 300 GTGCAC G[AT]GC[AT]^C
AviII 308 TGCGCA TGC^GCA
BcnI 263 CCGGG CC^[GC]GG
BsaHI 844 GACGCC G[GA]^CG[CT]C
BseAI 125 TCCGGA T^CCGGA
BsiHKAI 300 GTGCAC G[AT]GC[AT]^C
BspEI 125 TCCGGA T^CCGGA
BsrDI 337 CATTGC GCAATG(2/0)
BssHII 53 GCGCGC G^CGCGC
BstXI 1136 CCAAATGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DsaI 65 CCGTGG C^C[GA][CT]GG
FspI 308 TGCGCA TGC^GCA
HaeII 88 AGCGCC [GA]GCGC^[CT]
HgaI 844 GACGC GACGC(5/10)
Hin1I 844 GACGCC G[GA]^CG[CT]C
HindIII 448 AAGCTT A^AGCTT
HphI 288 TCACC GGTGA(8/7)
MaeII 20 ACGT A^CGT
MroI 125 TCCGGA T^CCGGA
MspA1I 1066 CAGCGG C[AC]G^C[GT]G
NciI 263 CCGGG CC^[GC]GG
NspI 999 GCATGT [GA]CATG^[CT]
PpuMI 398 AGGTCCT [GA]G^G[AT]CC[CT]
PshAI 1057 GACTGCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 354 CTGCAG CTGCA^G
RsaI 351 GTAC GT^AC
SexAI 395 ACCAGGT A^CC[AT]GGT
SfcI 354 CTGCAG C^T[GA][CT]AG
TaqI 875 TCGA T^CGA
TthHB8I 875 TCGA T^CGA
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AcsI 634 AAATTC [GA]^AATT[CT]
1037 AAATTT
ApoI 634 AAATTC [GA]^AATT[CT]
1037 AAATTT
BalI 521 TGGCCA TGG^CCA
756 TGGCCA
BfaI 229 CTAG C^TAG
512 CTAG
BsaWI 125 TCCGGA [AT]^CCGG[AT]
725 TCCGGT
BsgI 167 CTGCAC GTGCAG(16/14)
359 GTGCAG
BsmI 183 GAATGC GAATGC(1/-1)
246 GAATGC
BstYI 910 GGATCT [GA]^GATC[CT]
1097 AGATCC
EaeI 521 TGGCCA [CT]^GGCC[GA]
756 TGGCCA
EcoNI 681 CCTCTATGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1143 CCTGGCAGAGG
EcoT14I 92 CCAAGG C^C[AT][AT]GG
759 CCAAGG
MaeI 229 CTAG C^TAG
512 CTAG
MflI 910 GGATCT [GA]^GATC[CT]
1097 AGATCC
MluNI 521 TGGCCA TGG^CCA
756 TGGCCA
MscI 521 TGGCCA TGG^CCA
756 TGGCCA
MseI 899 TTAA T^TAA
1022 TTAA
PleI 563 GACTC GAGTC(4/5)
665 GACTC
StyI 92 CCAAGG C^C[AT][AT]GG
759 CCAAGG
TfiI 722 GATTC G^A[AT]TC
764 GAATC
Tru9I 899 TTAA T^TAA
1022 TTAA
XhoII 910 GGATCT [GA]^GATC[CT]
1097 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 65 enzymes) #
#########################################
AccII AciI AluI AlwI AlwNI
AocI AvaII BbvI BmyI BpmI
BsaI BsaJI BseRI BsiYI BslI
BsmAI BsmFI Bsp1286I BsrI BssKI
BstF5I BstNI BstUI Bsu36I Cac8I
CfoI Cfr13I DdeI DpnII DraII
EarI Eco81I EcoO109I EcoRII FauI
Fnu4HI FokI HaeIII HapII HhaI
HinP1I HinfI HpaII ItaI Ksp632I
MaeIII MboI MboII MnlI MslI
MspI MvaI MvnI MwoI NdeII
NlaIII NlaIV Sau3AI Sau96I ScrFI
SfaNI TseI Tsp45I Tsp509I TspRI