Restriction map for
pF1KB6666
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# NO CUT ENZYMES ( 144 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcyI AflII AgeI AhdI Alw44I
Aor51HI ApaI ApaLI AscI AseI
AsnI Asp700I Asp718I AspEI AspI
AvaI AviII AvrII BamHI BanI
BanII BbeI BbrPI BbvCI BcgI
BciVI BclI BfrI BglI BglII
BlnI BlpI Bpu1102I BsaAI BsaBI
BsaHI BsaI BsaWI BseAI BseRI
BsgI BsiEI BsiWI BsmBI BsmI
BsoBI Bsp1407I BspEI BspHI BspMI
BsrFI BsrGI BssHII BssSI Bst1107I
BstBI BstEII BstPI BstUI BstZ17I
CelII CfoI Cfr10I CpoI DraI
DraIII DrdI DsaI EagI Eam1105I
EclXI Eco47III Eco52I EcoNI EcoO65I
EcoRI FbaI FseI FspI HaeII
HhaI Hin1I HinP1I HindIII HphI
KasI KpnI KspI MaeII MamI
MluI MroI MvnI NaeI NarI
NcoI NgoMIV NheI NotI NruI
NspV PacI PaeR7I PflFI PflMI
PinAI PmaCI PmeI PmlI PshBI
Psp1406I PvuI RcaI RsrII SacI
SacII SalI SapI SbfI SexAI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI Sse8387I SspBI SspI
StuI SwaI Tth111I Van91I XbaI
XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 44 enzymes) #
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AflIII 67 ACATGT A^C[GA][CT]GT
AlwNI 1014 CAGTACCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 1345 CCTGAGG CC^T[ACGT]AGG
AspHI 1309 GAGCAC G[AT]GC[AT]^C
BalI 1101 TGGCCA TGG^CCA
BfaI 974 CTAG C^TAG
BmyI 1309 GAGCAC G[AGT]GC[ACT]^C
BpmI 935 CTGGAG CTGGAG(16/14)
BsiHKAI 1309 GAGCAC G[AT]GC[AT]^C
BsiYI 1278 CCACCTGGGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 1278 CCACCTGGGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
Bsp1286I 1309 GAGCAC G[AGT]GC[ACT]^C
BspLU11I 67 ACATGT A^CATGT
BsrBI 567 GAGCGG CCGCTC(-3/-3)
BstXI 1104 CCATCTCAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 1345 CCTGAGG CC^T[ACGT]AGG
DraII 879 GGGACCT [GA]G^G[ACGT]CC[CT]
EaeI 1101 TGGCCA [CT]^GGCC[GA]
Eco81I 1345 CCTGAGG CC^T[ACGT]AGG
EcoO109I 879 GGGACCT [GA]G^G[ACGT]CC[CT]
EcoRV 517 GATATC GAT^ATC
EcoT14I 1125 CCAAGG C^C[AT][AT]GG
EcoT22I 809 ATGCAT ATGCA^T
FauI 569 GCGGG CCCGC(4/6)
HgaI 316 GACGC GACGC(5/10)
HincII 442 GTTAAC GT[CT]^[GA]AC
HindII 442 GTTAAC GT[CT]^[GA]AC
HpaI 442 GTTAAC GTT^AAC
MaeI 974 CTAG C^TAG
MfeI 1256 CAATTG C^AATTG
MluNI 1101 TGGCCA TGG^CCA
MscI 1101 TGGCCA TGG^CCA
MslI 1105 CATCTCAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 253 CAGCTG C[AC]G^C[GT]G
MunI 1256 CAATTG C^AATTG
NsiI 809 ATGCAT ATGCA^T
PpuMI 879 GGGACCT [GA]G^G[AT]CC[CT]
PshAI 725 GACATCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 854 CTGCAG CTGCA^G
PvuII 253 CAGCTG CAG^CTG
ScaI 850 AGTACT AGT^ACT
SmlI 390 CTCAAG C^T[CT][GA]AG
SphI 1004 GCATGC GCATG^C
StyI 1125 CCAAGG C^C[AT][AT]GG
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# TWO CUT ENZYMES ( 31 enzymes) #
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AcsI 24 GAATTT [GA]^AATT[CT]
751 GAATTT
ApoI 24 GAATTT [GA]^AATT[CT]
751 GAATTT
AvaII 8 GGTCC G^G[AT]CC
880 GGACC
BbsI 208 GAAGAC GAAGAC(2/6)
829 GAAGAC
BbvI 101 GCTGC GCAGC(8/12)
319 GCTGC
BcnI 20 CCCGG CC^[GC]GG
1199 CCCGG
BpuAI 208 GAAGAC GAAGAC(2/6)
829 GAAGAC
BsmFI 879 GGGAC GGGAC(10/14)
1022 GGGAC
BspDI 372 ATCGAT AT^CGAT
628 ATCGAT
BsrDI 150 CATTGC GCAATG(2/0)
543 CATTGC
BstAPI 76 GCATATGTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
856 GCAGACTATGC
BstYI 192 GGATCT [GA]^GATC[CT]
1069 GGATCT
ClaI 372 ATCGAT AT^CGAT
628 ATCGAT
EarI 60 CTCTTC CTCTTC(1/4)
663 GAAGAG
Fnu4HI 101 GCTGC GC^[ACGT]GC
319 GCTGC
HaeIII 1102 GGCC GG^CC
1185 GGCC
HapII 21 CCGG C^CGG
1200 CCGG
HpaII 21 CCGG C^CGG
1200 CCGG
ItaI 101 GCTGC GC^[ACGT]GC
319 GCTGC
Ksp632I 60 CTCTTC CTCTTC(1/4)
663 GAAGAG
MaeIII 977 GTGAC ^GT[ACGT]AC
1094 GTCAC
MflI 192 GGATCT [GA]^GATC[CT]
1069 GGATCT
MnlI 589 CCTC CCTC(7/6)
1348 GAGG
MspI 21 CCGG C^CGG
1200 CCGG
NciI 20 CCCGG CC^[GC]GG
1199 CCCGG
NspI 67 ACATGT [GA]CATG^[CT]
1004 GCATGC
SfcI 196 CTACAG C^T[GA][CT]AG
854 CTGCAG
TseI 101 GCTGC G^C[AT]GC
319 GCTGC
Tsp45I 977 GTGAC ^GT[GC]AC
1094 GTCAC
XcmI 513 CCATGATATCACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
866 CCATGTTCAAGGTGG
XhoII 192 GGATCT [GA]^GATC[CT]
1069 GGATCT
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# 3 OR MORE CUT ENZYMES ( 39 enzymes) #
#########################################
AccI AciI AfaI AluI AlwI
BsaJI BsmAI BsrI BssKI BstF5I
BstNI Cac8I Cfr13I DdeI DpnII
EcoRII FokI HinfI MboI MboII
MseI MvaI MwoI NdeI NdeII
NlaIII NlaIV PleI RsaI Sau3AI
Sau96I ScrFI SfaNI TaqI TfiI
Tru9I Tsp509I TspRI TthHB8I