Restriction map for
pF1KB0962
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# NO CUT ENZYMES ( 140 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AflIII AgeI
AhdI Alw44I AocI Aor51HI ApaI
ApaLI AscI AseI AsnI Asp718I
AspEI AspI AviII AvrII BbeI
BbrPI BbvCI BclI BfrI BglII
BlnI BlpI Bpu1102I BsaAI BsaBI
BsaHI BsaI BsaWI BseAI BsiEI
BsiWI BsmBI BsmI BspDI BspEI
BspLU11I BspMI BsrBI BsrDI BsrFI
BssHII BssSI Bst1107I BstBI BstEII
BstPI BstUI BstZ17I Bsu36I CelII
Cfr10I ClaI CpoI DraI DraII
DrdI DsaI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoO109I EcoO65I
EcoRI FbaI FseI FspI HaeII
HgaI Hin1I HincII HindII HindIII
HpaI KasI KpnI KspI MamI
MfeI MluI MroI MseI MslI
MunI MvnI NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI
NspV PacI PaeR7I PflFI PflMI
PinAI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PvuI RsrII
SacII SalI SbfI ScaI SfiI
SfoI SfuI SgrAI SnaBI SpeI
SphI Sse8387I SspI StuI SwaI
Tru9I Tth111I Van91I XbaI XhoI
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# ONE CUT ENZYMES ( 41 enzymes) #
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AlwNI 733 CAGCTGCTG CAG[ACGT][ACGT][ACGT]^CTG
BalI 2 TGGCCA TGG^CCA
BamHI 539 GGATCC G^GATCC
BanI 290 GGCACC G^G[CT][GA]CC
BcgI 393 CGAAGAAGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 1173 GGATAC GTATCC(6/5)
BfaI 574 CTAG C^TAG
BglI 723 GCCACCCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsmFI 158 GTCCC GGGAC(10/14)
Bsp1407I 489 TGTACA T^GTACA
BspHI 621 TCATGA T^CATGA
BsrGI 489 TGTACA T^GTACA
BstAPI 714 GCAGAACATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 50 CCATCTGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CfoI 1195 GCGC GCG^C
EaeI 2 TGGCCA [CT]^GGCC[GA]
EcoNI 573 CCTAGAGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV 745 GATATC GAT^ATC
EcoT14I 359 CCAAGG C^C[AT][AT]GG
EcoT22I 114 ATGCAT ATGCA^T
FauI 637 GCGGG CCCGC(4/6)
HhaI 1195 GCGC GCG^C
HinP1I 1195 GCGC G^CGC
MaeI 574 CTAG C^TAG
MaeII 1190 ACGT A^CGT
MluNI 2 TGGCCA TGG^CCA
MscI 2 TGGCCA TGG^CCA
MspA1I 733 CAGCTG C[AC]G^C[GT]G
NsiI 114 ATGCAT ATGCA^T
PleI 850 GACTC GAGTC(4/5)
PvuII 733 CAGCTG CAG^CTG
RcaI 621 TCATGA T^CATGA
SapI 951 GAAGAGC GCTCTTC(1/4)
SexAI 515 ACCAGGT A^CC[AT]GGT
SmaI 1006 CCCGGG CCC^GGG
SmlI 682 CTCAAG C^T[CT][GA]AG
SspBI 489 TGTACA T^GTACA
StyI 359 CCAAGG C^C[AT][AT]GG
XcmI 728 CCAGGCAGCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 1006 CCCGGG C^CCGGG
XmaI 1006 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AfaI 469 GTAC GT^AC
490 GTAC
AspHI 1164 GAGCTC G[AT]GC[AT]^C
1207 GAGCTC
AvaI 285 CCCGAG C^[CT]CG[GA]G
1006 CCCGGG
AvaII 363 GGACC G^G[AT]CC
513 GGACC
BsgI 256 GTGCAG GTGCAG(16/14)
762 GTGCAG
BsiHKAI 1164 GAGCTC G[AT]GC[AT]^C
1207 GAGCTC
BsmAI 463 GAGAC GTCTC(1/5)
1124 GTCTC
BsoBI 285 CCCGAG C^[CT]CG[GA]G
1006 CCCGGG
BstF5I 1070 CATCC GGATG(2/0)
321 GGATG
BstYI 434 AGATCC [GA]^GATC[CT]
539 GGATCC
Cac8I 1021 GCAGGC GC[ACGT]^[ACGT]GC
1162 GCGAGC
DraIII 482 CACAAGGTG CAC[ACGT][ACGT][ACGT]^GTG
1220 CACTGAGTG
FokI 1070 CATCC GGATG(9/13)
321 GGATG
HapII 1007 CCGG C^CGG
1245 CCGG
HpaII 1007 CCGG C^CGG
1245 CCGG
HphI 80 TCACC GGTGA(8/7)
1053 TCACC
MflI 434 AGATCC [GA]^GATC[CT]
539 GGATCC
MspI 1007 CCGG C^CGG
1245 CCGG
NspI 280 ACATGC [GA]CATG^[CT]
719 ACATGC
PstI 67 CTGCAG CTGCA^G
1019 CTGCAG
RsaI 469 GTAC GT^AC
490 GTAC
SacI 1164 GAGCTC GAGCT^C
1207 GAGCTC
SfaNI 116 GCATC GCATC(5/9)
564 GCATC
SfcI 67 CTGCAG C^T[GA][CT]AG
1019 CTGCAG
Tsp45I 79 GTCAC ^GT[GC]AC
1108 GTCAC
XhoII 434 AGATCC [GA]^GATC[CT]
539 GGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 51 enzymes) #
#########################################
AciI AcsI AluI AlwI ApoI
Asp700I BanII BbsI BbvI BcnI
BmyI BpmI BpuAI BsaJI BseRI
BsiYI BslI Bsp1286I BsrI BssKI
BstNI Cfr13I DdeI DpnII EarI
EcoRII Fnu4HI HaeIII HinfI ItaI
Ksp632I MaeIII MboI MboII MnlI
MvaI MwoI NciI NdeII NlaIII
NlaIV Sau3AI Sau96I ScrFI TaqI
TfiI TseI Tsp509I TspRI TthHB8I
XmnI