##################################
# NO CUT ENZYMES (  197 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AciI        AclI        AcsI        AcyI        AflII       
AflIII      AgeI        AhdI        Alw44I      AlwNI       
AocI        Aor51HI     ApaI        ApaLI       ApoI        
AscI        AseI        AsnI        Asp700I     Asp718I     
AspEI       AspHI       AvaI        AviII       AvrII       
BalI        BanI        BanII       BbeI        BbrPI       
BbsI        BbvCI       BclI        BcnI        BfrI        
BglI        BglII       BlnI        BlpI        BmyI        
BpmI        Bpu1102I    BpuAI       BsaAI       BsaBI       
BsaHI       BsaWI       BseAI       BsiEI       BsiHKAI     
BsiWI       BsiYI       BslI        BsmBI       BsmFI       
BsmI        BsoBI       Bsp1286I    Bsp1407I    BspDI       
BspEI       BspHI       BspLU11I    BsrBI       BsrDI       
BsrFI       BsrGI       BssHII      BssSI       Bst1107I    
BstAPI      BstBI       BstEII      BstPI       BstUI       
BstXI       BstZ17I     Bsu36I      CelII       CfoI        
Cfr10I      ClaI        CpoI        DraI        DraIII      
EaeI        EagI        Eam1105I    EarI        EclXI       
Eco47III    Eco52I      Eco81I      EcoNI       EcoO65I     
EcoRI       EcoRV       EcoT22I     FauI        FbaI        
FseI        FspI        HaeII       HapII       HgaI        
HhaI        Hin1I       HinP1I      HindIII     HinfI       
HpaI        HpaII       KasI        KpnI        Ksp632I     
KspI        MaeII       MamI        MfeI        MluI        
MluNI       MroI        MscI        MslI        MspA1I      
MspI        MunI        MvnI        MwoI        NaeI        
NarI        NciI        NcoI        NdeI        NgoMIV      
NheI        NlaIII      NotI        NruI        NsiI        
NspI        NspV        PacI        PaeR7I      PflMI       
PinAI       PleI        PmaCI       PmeI        PmlI        
PpuMI       PshAI       PshBI       Psp1406I    PstI        
PvuI        PvuII       RcaI        RsrII       SacI        
SacII       SalI        SapI        SbfI        ScaI        
SexAI       SfaNI       SfcI        SfiI        SfoI        
SfuI        SgrAI       SmaI        SmlI        SnaBI       
SphI        Sse8387I    SspBI       SspI        StuI        
SwaI        TaqI        TfiI        TspRI       TthHB8I     
Van91I      XbaI        XcmI        XhoI        XmaCI       
XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   43 enzymes) #
###################################

AfaI          258  GTAC                 GT^AC
AluI          344  AGCT                 AG^CT
AspI          244  GACTGGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AvaII         249  GGTCC                G^G[AT]CC
BamHI         267  GGATCC               G^GATCC
BcgI          217  CGAGAAGTCTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI         157  GGATAC               GTATCC(6/5)
BfaI          148  CTAG                 C^TAG
BsaI            6  GGTCTC               GGTCTC(1/5)
BseRI         346  CTCCTC               GAGGAG(10/8)
BsgI          225  CTGCAC               GTGCAG(16/14)
BsrI          245  ACTGG                ACTGG(1/-1)
BssKI         293  CCAGG                ^CC[ACGT]GG
BstNI         293  CCAGG                CC^[AT]GG
BstYI         267  GGATCC               [GA]^GATC[CT]
DdeI           83  CTGAG                C^T[ACGT]AG
DpnII         268  GATC                 ^GATC
DraII         167  AGGCCCT              [GA]G^G[ACGT]CC[CT]
DrdI          241  GACGACTGGGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI          305  CCACGG               C^C[GA][CT]GG
EcoO109I      167  AGGCCCT              [GA]G^G[ACGT]CC[CT]
EcoRII        293  CCAGG                ^CC[AT]GG
EcoT14I       139  CCAAGG               C^C[AT][AT]GG
HaeIII        168  GGCC                 GG^CC
HincII        335  GTCAAC               GT[CT]^[GA]AC
HindII        335  GTCAAC               GT[CT]^[GA]AC
MaeI          148  CTAG                 C^TAG
MboI          268  GATC                 ^GATC
MboII         197  TCTTC                GAAGA(8/7)
MflI          267  GGATCC               [GA]^GATC[CT]
MseI          311  TTAA                 T^TAA
MvaI          293  CCAGG                CC^[AT]GG
NdeII         268  GATC                 ^GATC
PflFI         244  GACTGGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
RsaI          258  GTAC                 GT^AC
Sau3AI        268  GATC                 ^GATC
ScrFI         293  CCAGG                CC^[ACGT]GG
SpeI          147  ACTAGT               A^CTAGT
StyI          139  CCAAGG               C^C[AT][AT]GG
Tru9I         311  TTAA                 T^TAA
Tsp509I       315  AATT                 ^AATT
Tth111I       244  GACTGGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XhoII         267  GGATCC               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (    9 enzymes) #
###################################

AlwI          268  GATCC                GGATC(4/5)
              267  GGATC               
BspMI         106  ACCTGC               ACCTGC(4/8)
              182  ACCTGC              
BstF5I         39  CATCC                GGATG(2/0)
              101  CATCC               
Cac8I          68  GCCAGC               GC[ACGT]^[ACGT]GC
              186  GCCAGC              
Cfr13I        168  GGCCC                G^G[ACGT]CC
              249  GGTCC               
FokI           39  CATCC                GGATG(9/13)
              101  CATCC               
MaeIII        179  GTCAC                ^GT[ACGT]AC
              202  GTCAC               
Sau96I        168  GGCCC                G^G[ACGT]CC
              249  GGTCC               
Tsp45I        179  GTCAC                ^GT[GC]AC
              202  GTCAC               


#########################################
# 3 OR MORE CUT ENZYMES (    9 enzymes) #
#########################################

BbvI        BsaJI       BsmAI       Fnu4HI      HphI        
ItaI        MnlI        NlaIV       TseI