Restriction map for
pF1KB0965
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# NO CUT ENZYMES ( 178 enzymes) #
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AatII Acc65I AccIII AclI AcyI
AfaI AflII AgeI AhdI AluI
Alw44I AlwNI AocI Aor51HI ApaI
ApaLI AscI AseI AsnI Asp700I
Asp718I AspEI AspHI AspI AvaI
AvaII AviII AvrII BalI BamHI
BanI BanII BbeI BbrPI BbsI
BbvCI BciVI BclI BcnI BfaI
BfrI BglI BlnI BlpI Bpu1102I
BpuAI BsaAI BsaBI BsaHI BsaI
BsaWI BseAI BseRI BsgI BsiEI
BsiHKAI BsiWI BsmBI BsmI BsoBI
Bsp1407I BspDI BspEI BspLU11I BspMI
BsrBI BsrGI BssHII BssKI BssSI
Bst1107I BstBI BstEII BstF5I BstNI
BstPI BstZ17I Bsu36I CelII ClaI
CpoI DraI DraII DraIII DrdI
EaeI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoNI EcoO109I EcoO65I
EcoRI EcoRII EcoRV EcoT22I FauI
FbaI FokI FseI FspI HgaI
Hin1I HincII HindII HindIII HpaI
HphI KasI KpnI KspI MaeI
MamI MfeI MluI MluNI MroI
MscI MseI MslI MunI MvaI
NarI NciI NcoI NdeI NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PvuI PvuII RsaI RsrII
SacI SacII SalI SapI ScaI
ScrFI SexAI SfiI SfoI SfuI
SgrAI SmaI SmlI SnaBI SpeI
SphI SspBI SspI SwaI TfiI
Tru9I Tth111I XbaI XcmI XhoI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 46 enzymes) #
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AccI 49 GTCTAC GT^[AC][GT]AC
AccII 147 CGCG CG^CG
AflIII 97 ACGTGT A^C[GA][CT]GT
AlwI 209 GGATC GGATC(4/5)
BbvI 385 GCTGC GCAGC(8/12)
BcgI 137 GCACGACAGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglII 164 AGATCT A^GATCT
BmyI 357 GTGCCC G[AGT]GC[ACT]^C
BpmI 460 CTGGAG CTGGAG(16/14)
BsmAI 236 GTCTC GTCTC(1/5)
BsmFI 282 GTCCC GGGAC(10/14)
Bsp1286I 357 GTGCCC G[AGT]GC[ACT]^C
BspHI 153 TCATGA T^CATGA
BsrDI 74 GCAATG GCAATG(2/0)
BsrFI 291 GCCGGC [GA]^CCGG[CT]
BstUI 147 CGCG CG^CG
BstXI 69 CCAGTGCAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 164 AGATCT [GA]^GATC[CT]
Cfr10I 291 GCCGGC [GA]^CCGG[CT]
DsaI 533 CCACGG C^C[GA][CT]GG
EarI 453 GAAGAG CTCTTC(1/4)
EcoT14I 415 CCTTGG C^C[AT][AT]GG
HaeII 226 GGCGCT [GA]GCGC^[CT]
HapII 292 CCGG C^CGG
HpaII 292 CCGG C^CGG
Ksp632I 453 GAAGAG CTCTTC(1/4)
MaeII 97 ACGT A^CGT
MflI 164 AGATCT [GA]^GATC[CT]
MspA1I 435 CCGCTG C[AC]G^C[GT]G
MspI 292 CCGG C^CGG
MvnI 147 CGCG CG^CG
NaeI 291 GCCGGC GCC^GGC
NgoMIV 291 GCCGGC G^CCGGC
PflMI 105 CCACTGCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
RcaI 153 TCATGA T^CATGA
SbfI 116 CCTGCAGG CCTGCA^GG
SfaNI 258 GCATC GCATC(5/9)
Sse8387I 116 CCTGCAGG CCTGCA^GG
StuI 552 AGGCCT AGG^CCT
StyI 415 CCTTGG C^C[AT][AT]GG
TaqI 363 TCGA T^CGA
TseI 385 GCTGC G^C[AT]GC
Tsp45I 475 GTCAC ^GT[GC]AC
TthHB8I 363 TCGA T^CGA
Van91I 105 CCACTGCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 164 AGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 17 enzymes) #
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AcsI 325 AAATTC [GA]^AATT[CT]
522 AAATTT
ApoI 325 AAATTC [GA]^AATT[CT]
522 AAATTT
BsaJI 415 CCTTGG C^C[ACGT][ACGT]GG
533 CCACGG
BsrI 8 ACTGG ACTGG(1/-1)
69 CCAGT
Cfr13I 199 GGCCC G^G[ACGT]CC
529 GGCCC
DpnII 165 GATC ^GATC
210 GATC
Fnu4HI 148 GCGGC GC^[ACGT]GC
385 GCTGC
ItaI 148 GCGGC GC^[ACGT]GC
385 GCTGC
MaeIII 304 GTTAC ^GT[ACGT]AC
475 GTCAC
MboI 165 GATC ^GATC
210 GATC
MboII 61 GAAGA GAAGA(8/7)
453 GAAGA
NdeII 165 GATC ^GATC
210 GATC
PstI 117 CTGCAG CTGCA^G
268 CTGCAG
Sau3AI 165 GATC ^GATC
210 GATC
Sau96I 199 GGCCC G^G[ACGT]CC
529 GGCCC
SfcI 117 CTGCAG C^T[GA][CT]AG
268 CTGCAG
Tsp509I 326 AATT ^AATT
523 AATT
#########################################
# 3 OR MORE CUT ENZYMES ( 17 enzymes) #
#########################################
AciI BsiYI BslI BstAPI Cac8I
CfoI DdeI HaeIII HhaI HinP1I
HinfI MnlI MwoI NlaIII NlaIV
PleI TspRI