Restriction map for
pF1KB0968
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# NO CUT ENZYMES ( 134 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AflII AflIII AgeI AhdI Alw44I
AlwI ApaLI AscI AseI AsnI
Asp718I AspEI AspHI AspI AvrII
BamHI BbeI BbrPI BbsI BbvCI
BcgI BciVI BclI BfrI BglII
BlnI BpuAI BsaAI BsaI BsaWI
BseAI BsiEI BsiHKAI BsiWI BsmAI
BsmBI BsmI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrDI BsrFI BsrGI
BssHII BssSI Bst1107I BstBI BstEII
BstPI BstUI BstYI BstZ17I Cfr10I
ClaI CpoI DraI DrdI EagI
Eam1105I EclXI Eco52I EcoO65I EcoRI
EcoRV EcoT22I FbaI FseI HincII
HindII HindIII HpaI KasI KpnI
KspI MaeII MfeI MflI MluI
MroI MunI MvnI NaeI NarI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SacI SacII SalI ScaI
SexAI SfiI SfoI SfuI SgrAI
SnaBI SphI SspBI SspI StuI
SwaI TaqI TfiI Tth111I TthHB8I
Van91I XbaI XhoI XhoII
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# ONE CUT ENZYMES ( 47 enzymes) #
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AccI 373 GTCTAC GT^[AC][GT]AC
AcsI 76 AAATTT [GA]^AATT[CT]
AlwNI 851 CAGCGGCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 254 CCTCAGG CC^T[ACGT]AGG
Aor51HI 1082 AGCGCT AGC^GCT
ApoI 76 AAATTT [GA]^AATT[CT]
Asp700I 1053 GAAGAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AviII 1034 TGCGCA TGC^GCA
BalI 50 TGGCCA TGG^CCA
BanI 310 GGCACC G^G[CT][GA]CC
BglI 336 GCCCAAGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 559 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 559 GCTCAGC GC^T[ACGT]AGC
BsaBI 184 GATGCTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsgI 69 GTGCAG GTGCAG(16/14)
BsrBI 835 GAGCGG CCGCTC(-3/-3)
BstAPI 663 GCATGTCTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Bsu36I 254 CCTCAGG CC^T[ACGT]AGG
CelII 559 GCTCAGC GC^T[ACGT]AGC
DpnII 1290 GATC ^GATC
DraIII 746 CACGGGGTG CAC[ACGT][ACGT][ACGT]^GTG
EaeI 50 TGGCCA [CT]^GGCC[GA]
Eco47III 1082 AGCGCT AGC^GCT
Eco81I 254 CCTCAGG CC^T[ACGT]AGG
EcoNI 12 CCTGCTTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FauI 99 GCGGG CCCGC(4/6)
FspI 1034 TGCGCA TGC^GCA
HinfI 103 GACTC G^A[ACGT]TC
MamI 184 GATGCTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MboI 1290 GATC ^GATC
MluNI 50 TGGCCA TGG^CCA
MscI 50 TGGCCA TGG^CCA
MseI 614 TTAA T^TAA
MslI 397 CACCCCTATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NdeII 1290 GATC ^GATC
NspI 663 GCATGT [GA]CATG^[CT]
PleI 103 GACTC GAGTC(4/5)
PstI 230 CTGCAG CTGCA^G
Sau3AI 1290 GATC ^GATC
SbfI 229 CCTGCAGG CCTGCA^GG
SfcI 230 CTGCAG C^T[GA][CT]AG
SmlI 16 CTTGAG C^T[CT][GA]AG
SpeI 330 ACTAGT A^CTAGT
Sse8387I 229 CCTGCAGG CCTGCA^GG
Tru9I 614 TTAA T^TAA
XcmI 288 CCACTCATTCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 1053 GAAGAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AcyI 3 GGCGTC G[GA]^CG[CT]C
1212 GACGCC
AfaI 141 GTAC GT^AC
414 GTAC
ApaI 301 GGGCCC GGGCC^C
655 GGGCCC
AvaI 1046 CCCGGG C^[CT]CG[GA]G
1140 CCCGGG
BsaHI 3 GGCGTC G[GA]^CG[CT]C
1212 GACGCC
BsoBI 1046 CCCGGG C^[CT]CG[GA]G
1140 CCCGGG
BspMI 228 ACCTGC ACCTGC(4/8)
1063 ACCTGC
HaeII 797 GGCGCT [GA]GCGC^[CT]
1082 AGCGCT
HapII 1047 CCGG C^CGG
1141 CCGG
Hin1I 3 GGCGTC G[GA]^CG[CT]C
1212 GACGCC
HpaII 1047 CCGG C^CGG
1141 CCGG
MaeIII 155 GTGAC ^GT[ACGT]AC
1097 GTGAC
MspI 1047 CCGG C^CGG
1141 CCGG
NcoI 176 CCATGG C^CATGG
469 CCATGG
PpuMI 317 AGGACCC [GA]G^G[AT]CC[CT]
1143 GGGTCCC
PvuII 580 CAGCTG CAG^CTG
882 CAGCTG
RsaI 141 GTAC GT^AC
414 GTAC
SapI 350 GCTCTTC GCTCTTC(1/4)
984 GCTCTTC
SfaNI 184 GATGC GCATC(5/9)
425 GCATC
SmaI 1046 CCCGGG CCC^GGG
1140 CCCGGG
Tsp45I 155 GTGAC ^GT[GC]AC
1097 GTGAC
Tsp509I 77 AATT ^AATT
820 AATT
XmaCI 1046 CCCGGG C^CCGGG
1140 CCCGGG
XmaI 1046 CCCGGG C^CCGGG
1140 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 53 enzymes) #
#########################################
AciI AluI AvaII BanII BbvI
BcnI BfaI BmyI BpmI BsaJI
BseRI BsiYI BslI BsmFI Bsp1286I
BsrI BssKI BstF5I BstNI BstXI
Cac8I CfoI Cfr13I DdeI DraII
DsaI EarI EcoO109I EcoRII EcoT14I
Fnu4HI FokI HaeIII HgaI HhaI
HinP1I HphI ItaI Ksp632I MaeI
MboII MnlI MspA1I MvaI MwoI
NciI NlaIII NlaIV Sau96I ScrFI
StyI TseI TspRI