Restriction map for
pF1KB0970
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# NO CUT ENZYMES ( 159 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AcyI AflII AflIII AgeI Alw44I
AlwNI AocI Aor51HI ApaI ApaLI
AscI Asp700I Asp718I AspHI AspI
AvaI AviII AvrII BbeI BbrPI
BbvCI BcgI BciVI BcnI BfrI
BglI BglII BlnI BlpI Bpu1102I
BsaAI BsaBI BsaHI BsaI BsaWI
BseAI BseRI BsiEI BsiHKAI BsiWI
BsmBI BsmI BsoBI Bsp1407I BspDI
BspEI BspHI BspLU11I BspMI BsrBI
BsrFI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstUI
BstXI BstZ17I Bsu36I CelII Cfr10I
ClaI CpoI DraI DraIII DrdI
DsaI EagI EarI EclXI Eco47III
Eco52I Eco81I EcoNI EcoO65I EcoRI
EcoT14I EcoT22I FseI FspI HaeII
HapII HgaI Hin1I HincII HindII
HindIII HpaI HpaII KasI KpnI
Ksp632I KspI MamI MfeI MluI
MroI MslI MspI MunI MvnI
NaeI NarI NciI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PshAI PstI
PvuI RcaI RsrII SacI SacII
SalI SapI SbfI ScaI SexAI
SfaNI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspBI SspI StyI SwaI Tth111I
XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 39 enzymes) #
###################################
AclI 1244 AACGTT AA^CGTT
AcsI 384 AAATTT [GA]^AATT[CT]
AfaI 1128 GTAC GT^AC
AhdI 118 GACATCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApoI 384 AAATTT [GA]^AATT[CT]
AseI 190 ATTAAT AT^TAAT
AsnI 190 ATTAAT AT^TAAT
AspEI 118 GACATCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaII 1163 GGTCC G^G[AT]CC
BamHI 529 GGATCC G^GATCC
BanI 924 GGCACC G^G[CT][GA]CC
BanII 157 GAGCCC G[GA]GC[CT]^C
BpmI 544 CTGGAG CTGGAG(16/14)
BsgI 1016 CTGCAC GTGCAG(16/14)
BsmAI 506 GAGAC GTCTC(1/5)
CfoI 673 GCGC GCG^C
Cfr13I 1163 GGTCC G^G[ACGT]CC
DraII 1162 GGGTCCC [GA]G^G[ACGT]CC[CT]
Eam1105I 118 GACATCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO109I 1162 GGGTCCC [GA]G^G[ACGT]CC[CT]
EcoRV 493 GATATC GAT^ATC
FauI 730 CCCGC CCCGC(4/6)
HhaI 673 GCGC GCG^C
HinP1I 673 GCGC G^CGC
MaeII 1245 ACGT A^CGT
MspA1I 787 CAGCTG C[AC]G^C[GT]G
NspI 232 GCATGT [GA]CATG^[CT]
PflMI 882 CCAAGCAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 1162 GGGTCCC [GA]G^G[AT]CC[CT]
PshBI 190 ATTAAT AT^TAAT
Psp1406I 1244 AACGTT AA^CGTT
PvuII 787 CAGCTG CAG^CTG
RsaI 1128 GTAC GT^AC
Sau96I 1163 GGTCC G^G[ACGT]CC
SfcI 851 CTACAG C^T[GA][CT]AG
StuI 1197 AGGCCT AGG^CCT
Van91I 882 CCAAGCAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 99 TCTAGA T^CTAGA
XcmI 1138 CCATTTGAAAAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
BalI 429 TGGCCA TGG^CCA
791 TGGCCA
BbsI 181 GAAGAC GAAGAC(2/6)
1041 GTCTTC
BclI 633 TGATCA T^GATCA
1268 TGATCA
BmyI 157 GAGCCC G[AGT]GC[ACT]^C
575 GTGCCC
BpuAI 181 GAAGAC GAAGAC(2/6)
1041 GTCTTC
Bsp1286I 157 GAGCCC G[AGT]GC[ACT]^C
575 GTGCCC
BsrDI 152 GCAATG GCAATG(2/0)
886 GCAATG
BstF5I 120 CATCC GGATG(2/0)
564 GGATG
BstYI 529 GGATCC [GA]^GATC[CT]
1277 GGATCT
Cac8I 729 GCCCGC GC[ACGT]^[ACGT]GC
789 GCTGGC
EaeI 429 TGGCCA [CT]^GGCC[GA]
791 TGGCCA
FbaI 633 TGATCA T^GATCA
1268 TGATCA
FokI 120 CATCC GGATG(9/13)
564 GGATG
HphI 1191 GGTGA GGTGA(8/7)
540 TCACC
MflI 529 GGATCC [GA]^GATC[CT]
1277 GGATCT
MluNI 429 TGGCCA TGG^CCA
791 TGGCCA
MscI 429 TGGCCA TGG^CCA
791 TGGCCA
SmlI 176 CTCAAG C^T[CT][GA]AG
1251 CTCAAG
XhoII 529 GGATCC [GA]^GATC[CT]
1277 GGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 41 enzymes) #
#########################################
AciI AluI AlwI BbvI BfaI
BsaJI BsiYI BslI BsmFI BsrI
BssKI BstNI DdeI DpnII EcoRII
Fnu4HI HaeIII HinfI ItaI MaeI
MaeIII MboI MboII MnlI MseI
MvaI MwoI NdeII NlaIII NlaIV
PleI Sau3AI ScrFI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I